Kuku Adenike
Department of Biochemistry, Obafemi Awolowo University, Ile-lfe, Nigeria
Agboola Femi Kayode
Department of Biochemistry, Obafemi Awolowo University, Ile-lfe, Nigeria
Aboderin Akintola
Department of Biochemistry, University of Uyo, Uyo, Nigeria
ABSTRACT
A heamagglutinating protein from the phosphate buffered saline (PBS) extract of the seeds of Psophocarpus palustris was purified by gel filtration on Sephadex G-150 column. The purified lectin agglutinated specifically red blood cells of human type A and neither type B nor type O. This heamagglutinating activity was inhibited by D-mannose and D-galactose. The purified protein showed one band in non-denaturing polyacrylamide gel electrophoresis (PAGE) as well as in SDS-PAGE. This revealed that the lectin is a homogeneous preparation and a dimeric protein with a molecular weight estimated from gel filtration of 45,000 daltons and subunit molecular weight of 22,700 daltons. Treatment of the lectin with 50 mM EDTA or EGTA had no influence on the hemagglutinating activity but Mg2+, Mn2+ and Ba2+ were effective activators of the purified lectin. The optimal pH for heamagglutination was 7. The purified lectin was stable in pH 7-9 but labile at temperatures over 50°C.
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How to cite this article
Kuku Adenike, Agboola Femi Kayode and Aboderin Akintola, 2005. Purification and Characterization of a Lectin from the Seeds of Psophocarpus palustris. Pakistan Journal of Biological Sciences, 8: 1667-1671.
DOI: 10.3923/pjbs.2005.1667.1671
URL: https://scialert.net/abstract/?doi=pjbs.2005.1667.1671
DOI: 10.3923/pjbs.2005.1667.1671
URL: https://scialert.net/abstract/?doi=pjbs.2005.1667.1671
REFERENCES
- Gornall, A.G., C.J. Bardawill and M.M. David, 1949. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem., 177: 751-766.
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