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Production and Some Properties of Protease Produced by Bacillus licheniformis Isolated from Tihamet Aseer, Saudi Arabia



Al- Shehri, M. Abdulrahman and Mostafa S. Yasser
 
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ABSTRACT

Culture conditions affecting protease production by Bacillus licheniformis isolated from Tihamet Aseer, Saudi Arabia were investigated. The bacterium strain gave a maximum protease production of 221 U mL-1 when growing in a casein broth medium after 36 h compared with gelatin broth medium. Optimum pH and temperature of protease production were 8 and 50°C, respectively. The highest level of protease production in the presence of soybean meal as a carbon source and the peptone as a nitrogen source was obtained. The protease was optimally active at pH 9 and 55°C . The enzyme was stable at temperature range of 60-65°C during the period tested (1h) and retaining more than 85% of its activity at 70°C. These properties make the enzyme suitable for detergent industry.

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  How to cite this article:

Al- Shehri, M. Abdulrahman and Mostafa S. Yasser , 2004. Production and Some Properties of Protease Produced by Bacillus licheniformis Isolated from Tihamet Aseer, Saudi Arabia. Pakistan Journal of Biological Sciences, 7: 1631-1635.

DOI: 10.3923/pjbs.2004.1631.1635

URL: https://scialert.net/abstract/?doi=pjbs.2004.1631.1635

REFERENCES
1:  Kulp, K., 1975. Enzyme in Food Processing. 2nd Edn., Academic Press, New York, London, pp: 53-122.

2:  Moon, S. and H. Parulekar, 1991. A parametric study of protease production in batch and fed-batch cultures of Bacillus firmus. Biotechnol. Bioeng., 37: 467-483.

3:  Feng, Y.Y., W.B. Yang, S.L. Ong, J.Y. Hu and W.J. Nig, 2001. Fermentation of starch for enhanced alkaline protease production by constructing an alkalophilic Bacillus pumilus strain. Applied Microbiol. Biotechnol., 57: 153-160.
Direct Link  |  

4:  Gupta, R., Q. Beg, S. Khan and B. Chauhan, 2002. An overview on fermentation, downstream processing of microbial alkaline proteases. Applied Microbiol. Biotechnol., 60: 381-395.
Direct Link  |  

5:  Chu, M., C. Lee and T. Li, 1992. Production and degradation of alkaline protease in batch cultures of Bacillus subtilis ATCC 14416. Enzyme Microb. Technol., 14: 755-761.

6:  Perez, M., R. Bocourt, J. Galindo, G. Milian, R. Paid, R. Alonso and G. Alfonso, 1999. Isolation and selection of Bacillus sp. strains producing proteolytic enzymes. Cuban J. Agric. Sci., 33: 401-408.

7:  Hagspiel, K., D. Haab and C. Kubicek, 1989. Protease activity and proteolytic degradation of cellulases in Trichoderma reesei. Applied Microb. Biotechnol., 32: 61-67.

8:  Singh, A., K. Ghosh and P. Ghosh, 1994. Production of thermostable acid protease by Aspergillus niger. Lett. Applied Microbiol., 18: 177-180.

9:  Xu, J., L. Wang, D. Ridgway, T. Gu and M.M. Young, 2000. Increased heterologous protein production in Aspergillus niger fermentation through extracellular proteases inhibition by pelleted growth. Biotechnol. Prog., 16: 222-227.

10:  Johnveshy, B. and G. Naik, 2001. Production of bleach-stable and halo-tolerant alkaline protease by an alkalophilic Bacillus pumilus JB 05 isolated from cement industry effluents. J. Microb. Biotechnol., 11: 558-563.

11:  Calik, P., E. Bilir, G. Calik and T.H. Ozdamar, 2002. Influence of pH conditions on metabolic regulations in serine alkaline protease production by Bacillus licheniformis. Enzyme Microbial. Technol., 31: 685-697.
CrossRef  |  Direct Link  |  

12:  Ali, O.A., 1992. Formation of proteases by Aspergillus fumigatus and Penicillium sp. J. King Saud Univ., 4: 127-136.

13:  Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Stanley and S.T. Williams, 1994. Bergeys Manual of Determinative Bacteriology. 9th Edn., Williams and Wilkins, Baltimore, USA..

14:  Chopra, A.K. and D.K. Mathur, 1985. Purification and characterization of heat-stable protease from Bacillus stearothermophilus RM-67. J. Dairy Sci., 68: 3202-3211.

15:  Schaffer, P., 1969. Sporulation and the production of antibiotics, enzymes and exotoxins. Bacteriol. Rev., 33: 48-57.

16:  Beg, Q., R. Saxena and R. Gupta, 2002. De-repression and subsequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations. Process. Biochem., 37: 1103-1109.

17:  MacFarlane, G., S. MacFarlane and G. Gibson, 1992. Synthesis and release of protease by Bacteroide fragilis. Curr. Microbiol., 24: 55-59.

18:  Kim, J.M., W.J. Lim and H.J. Suh, 2001. Feather-degrading Bacillus species from poultry waste. Process. Biochem., 37: 287-291.
CrossRef  |  

19:  El-Hawary, F.I. and I.I. Ibrahim, 1992. Comparative study on protease of three thermophilic Bacilli. Zagazig J. Agric. Res., 19: 777-787.

20:  Joo, H.S., C.G. Kumar, G.C. Park, K.T. Kim, S.R. Paik and C.S. Chang, 2002. Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii. Process Biochem., 38: 155-159.
CrossRef  |  

21:  Atalo, K. and B. Gashe, 1993. Protease production by thermophilic Bacillus species which degrades various kinds of fibrous proteins. Biotechnol. Lett., 15: 1151-1156.

22:  Johnvesly, B., B. Manjunath and G. Naik, 2002. Pigeon pea waste as a novel, inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophic Bacillus sp. JB-99. Bioresour. Technol., 82: 61-64.

23:  Nilegaonkar, S., P. Kanekar, S. Sarnaik and A. Kelkar, 2002. Production, isolation and characterization of extracellular protease of an alkaliphilic strain of Arthrobacter ramosus, MCM B-351 isolated form the alkaline lake of lonar, India. World J. Microb. Biotechnol., 18: 785-789.
Direct Link  |  

24:  Towatana, N., A. Painupong and P. Suntinanalert, 1999. Purification and characterization of an extracellular protease from alkaliphilic and thermophilic Bacillus sp. PS 719. J. Biosci. Bioeng., 87: 581-587.

25:  Gaur, R., J. Yadav and L. Pandey, 1989. Thermostability of extracellular protease enzyme produced by Spicaria fusispora, a thermophilic fungus. Hindustan Antibiotics, 31: 36-37.
PubMed  |  

26:  Whittle, G. and G. Bloomfield, 1999. The site-specific integration of genetic elements may nodulate thermostable protease production. Microbiology, 145: 2845-2851.

27:  Kanekar, P., S. Nilegaonkar, S. Sarnaik and A. Kelkar, 2002. Optimization of protease activity of alkaliphic bacteria isolated from an alkaline lake in India. Bioresour. Technol., 85: 87-93.

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