Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Research Article
 

Production and Some Properties of Protease Produced by Bacillus licheniformis Isolated from Tihamet Aseer, Saudi Arabia



Al- Shehri, M. Abdulrahman and Mostafa S. Yasser
 
ABSTRACT

Culture conditions affecting protease production by Bacillus licheniformis isolated from Tihamet Aseer, Saudi Arabia were investigated. The bacterium strain gave a maximum protease production of 221 U mL-1 when growing in a casein broth medium after 36 h compared with gelatin broth medium. Optimum pH and temperature of protease production were 8 and 50°C, respectively. The highest level of protease production in the presence of soybean meal as a carbon source and the peptone as a nitrogen source was obtained. The protease was optimally active at pH 9 and 55°C . The enzyme was stable at temperature range of 60-65°C during the period tested (1h) and retaining more than 85% of its activity at 70°C. These properties make the enzyme suitable for detergent industry.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Al- Shehri, M. Abdulrahman and Mostafa S. Yasser , 2004. Production and Some Properties of Protease Produced by Bacillus licheniformis Isolated from Tihamet Aseer, Saudi Arabia. Pakistan Journal of Biological Sciences, 7: 1631-1635.

DOI: 10.3923/pjbs.2004.1631.1635

URL: https://scialert.net/abstract/?doi=pjbs.2004.1631.1635

REFERENCES
Ali, O.A., 1992. Formation of proteases by Aspergillus fumigatus and Penicillium sp. J. King Saud Univ., 4: 127-136.

Atalo, K. and B. Gashe, 1993. Protease production by thermophilic Bacillus species which degrades various kinds of fibrous proteins. Biotechnol. Lett., 15: 1151-1156.

Beg, Q., R. Saxena and R. Gupta, 2002. De-repression and subsequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations. Process. Biochem., 37: 1103-1109.

Calik, P., E. Bilir, G. Calik and T.H. Ozdamar, 2002. Influence of pH conditions on metabolic regulations in serine alkaline protease production by Bacillus licheniformis. Enzyme Microbial. Technol., 31: 685-697.
CrossRef  |  Direct Link  |  

Chopra, A.K. and D.K. Mathur, 1985. Purification and characterization of heat-stable protease from Bacillus stearothermophilus RM-67. J. Dairy Sci., 68: 3202-3211.

Chu, M., C. Lee and T. Li, 1992. Production and degradation of alkaline protease in batch cultures of Bacillus subtilis ATCC 14416. Enzyme Microb. Technol., 14: 755-761.

El-Hawary, F.I. and I.I. Ibrahim, 1992. Comparative study on protease of three thermophilic Bacilli. Zagazig J. Agric. Res., 19: 777-787.

Feng, Y.Y., W.B. Yang, S.L. Ong, J.Y. Hu and W.J. Nig, 2001. Fermentation of starch for enhanced alkaline protease production by constructing an alkalophilic Bacillus pumilus strain. Applied Microbiol. Biotechnol., 57: 153-160.
Direct Link  |  

Gaur, R., J. Yadav and L. Pandey, 1989. Thermostability of extracellular protease enzyme produced by Spicaria fusispora, a thermophilic fungus. Hindustan Antibiotics, 31: 36-37.
PubMed  |  

Gupta, R., Q. Beg, S. Khan and B. Chauhan, 2002. An overview on fermentation, downstream processing of microbial alkaline proteases. Applied Microbiol. Biotechnol., 60: 381-395.
Direct Link  |  

Hagspiel, K., D. Haab and C. Kubicek, 1989. Protease activity and proteolytic degradation of cellulases in Trichoderma reesei. Applied Microb. Biotechnol., 32: 61-67.

Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Stanley and S.T. Williams, 1994. Bergeys Manual of Determinative Bacteriology. 9th Edn., Williams and Wilkins, Baltimore, USA..

Johnveshy, B. and G. Naik, 2001. Production of bleach-stable and halo-tolerant alkaline protease by an alkalophilic Bacillus pumilus JB 05 isolated from cement industry effluents. J. Microb. Biotechnol., 11: 558-563.

Johnvesly, B., B. Manjunath and G. Naik, 2002. Pigeon pea waste as a novel, inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophic Bacillus sp. JB-99. Bioresour. Technol., 82: 61-64.

Joo, H.S., C.G. Kumar, G.C. Park, K.T. Kim, S.R. Paik and C.S. Chang, 2002. Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii. Process Biochem., 38: 155-159.
CrossRef  |  

Kanekar, P., S. Nilegaonkar, S. Sarnaik and A. Kelkar, 2002. Optimization of protease activity of alkaliphic bacteria isolated from an alkaline lake in India. Bioresour. Technol., 85: 87-93.

Kim, J.M., W.J. Lim and H.J. Suh, 2001. Feather-degrading Bacillus species from poultry waste. Process. Biochem., 37: 287-291.
CrossRef  |  

Kulp, K., 1975. Enzyme in Food Processing. 2nd Edn., Academic Press, New York, London, pp: 53-122.

MacFarlane, G., S. MacFarlane and G. Gibson, 1992. Synthesis and release of protease by Bacteroide fragilis. Curr. Microbiol., 24: 55-59.

Moon, S. and H. Parulekar, 1991. A parametric study of protease production in batch and fed-batch cultures of Bacillus firmus. Biotechnol. Bioeng., 37: 467-483.

Nilegaonkar, S., P. Kanekar, S. Sarnaik and A. Kelkar, 2002. Production, isolation and characterization of extracellular protease of an alkaliphilic strain of Arthrobacter ramosus, MCM B-351 isolated form the alkaline lake of lonar, India. World J. Microb. Biotechnol., 18: 785-789.
Direct Link  |  

Perez, M., R. Bocourt, J. Galindo, G. Milian, R. Paid, R. Alonso and G. Alfonso, 1999. Isolation and selection of Bacillus sp. strains producing proteolytic enzymes. Cuban J. Agric. Sci., 33: 401-408.

Schaffer, P., 1969. Sporulation and the production of antibiotics, enzymes and exotoxins. Bacteriol. Rev., 33: 48-57.

Singh, A., K. Ghosh and P. Ghosh, 1994. Production of thermostable acid protease by Aspergillus niger. Lett. Applied Microbiol., 18: 177-180.

Towatana, N., A. Painupong and P. Suntinanalert, 1999. Purification and characterization of an extracellular protease from alkaliphilic and thermophilic Bacillus sp. PS 719. J. Biosci. Bioeng., 87: 581-587.

Whittle, G. and G. Bloomfield, 1999. The site-specific integration of genetic elements may nodulate thermostable protease production. Microbiology, 145: 2845-2851.

Xu, J., L. Wang, D. Ridgway, T. Gu and M.M. Young, 2000. Increased heterologous protein production in Aspergillus niger fermentation through extracellular proteases inhibition by pelleted growth. Biotechnol. Prog., 16: 222-227.

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract