Feeding Habits of Larval Fishes of the Family Clupeidae (Actinopterygii: Clupeiformes) in the Estuary of River Pendas, Johor, Malaysia
Studies on feeding habits and seasonal variation of diet of fish larvae of family Clupeidae was conducted from October 2007 to September 2008 in the estuary of Sg. Pendas, Gelang Patah, Johor, Malaysia. The diet composition were grouped into seven major categories consisted of phytoplankton, zooplankton, plant-like matter, debris, fragment of copepod, algae and unidentified food items. The most predominant food items in the gut of clupeids was phytoplankton (82.53%), followed by plant-like matter (7.34%), debris (4.86%), fragment of copepods (2.69%), algae (0.92%), unidentified items (0.77%) and zooplankton (0.54%).
Received: August 30, 2011;
Accepted: November 27, 2011;
Published: December 30, 2011
The fish from family Clupeidae is a member of the order Clupeiformes that are regarded as herring-like fish or clupeids. Herrings, shads, sardines, pilchards and menhaden are moderate-sized fishes under this group. The group includes a broad variety of species that lives in different habitat ranging from freshwater to marine. There are about 57 genera and 188 species world-wide under the family Clupeidae. These fishes usually have modified scales on the belly forming abdominal scutes with a saw-like edge. Most species have 2 long rod-like post-cleithra. The lateral line is usually absent or on only a few scales. Silvery cycloid scales are easily detached and are found only on the body. The mouth is usually terminal with jaws about equal in length. Teeth are small or absent but gill rakers are long and numerous for sieving plankton. Fins lack spines and there are no barbels. There is no adipose fin. The pectoral and pelvic fins have a large axillary scale. The caudal fin is deeply forked. The eye is partly covered by an adipose eyelid. The flesh is particularly oily and is highly nutritional. Members of this family often form immense schools in surface waters of the ocean and they feed on plankton. Usually, the larvae occupy different habitats than do the adults. The larvae can be found in the open waters of the continental shelf, older larvae may be found near the shore and estuarine channels and the juveniles occur in nurseries of estuarine sea grass beds.
Whitehead (1985) stated that clupeids are found in
most shallow water habitats including fresh water body, brackish estuaries,
coastal embayment and oceanic reefs. The clupeids are abundant and valuable
food fishes. Some of them are of economic importance such as the anchovies and
the wolf-herring. Ikan terubok ikan parang and ikan tamban are examples of fish
in Clupeidae family that are common in Malaysian waters (Chua
and Mathias, 1978). The clupeids Tenulosa toli (ikan terubok) is
potentially cultured species and commercially important tropical shad of the
estuaries in Sarawak (Blabber et al., 1997).
Detailed study on fish larvae has not been attempted so far except the studies
carried out by Ara et al. (2011) and Arshad
et al. (2011). Some reports are available on the food and feeding
habits of different fishes (Bhuiyan and Islam 1988;
Bhuiyan et al., 2006; Chrisfi
et al., 2007; Dadzie et al., 2000;
Jardas et al., 2007; Ara
et al., 2009, 2010) but little is known about
diet of the fish larvae from family Clupeidae, especially for clupeid larvae
in Malaysian waters. Therefore, the present study was undertaken to investigate
the feeding habits and diet composition of clupeids larvae in the estuary of
river Pendas, Gelang Patah, Johor.
MATERIALS AND METHODS
Study area and sampling: Monthly samples of fish larvae were collected from the estuary of river Pendas, Gelang Patah, Johor (Fig. 1) from October 2007 to September 2008. Specimens were collected using Bongo net with mesh size of 500 μm. Larval specimens were preserved in 5% formalin solution and transported to the laboratory for further study.
Stomach examination: Clupeids fish larvae (Fig. 2)
were sorted from other larval specimens. The Total Length (TL) and Body Length
(BL) were measured using Keyence Digital Microscope (VHX-500). In total, 64
stomach sacs were removed and diet compositions were examined during the twelve
months study period. The stomach was carefully removed from the body under the
|| Geographical location of the estuary of Pendas river, Gelang
Patah, Johor, Malaysia
|| Biological sketch of a Clupeidae larva
The stomach was later incised open onto a slide. A drop of distilled water
was dripped on it and covered with a cover glass. The gut contents were counted
and identified under a compound microscope to the lowest taxonomic group as
Stomach content analysis: To analyze the composition of the stomach,
percentage frequency of occurrence was followed (Chrisfi
et al., 2007) which as:
Percentage frequency of occurrence (Fpi)
= (N1i/Np) x100
where, N1i is the number of the stomachs in which food item
I was found and Np is the number of non-empty stomachs.
Diet composition: Prey analyses of stomach contents identified 25 important items belonging to seven major taxa groups: Phytoplankton, zooplankton, algae, insects, plant-like matter, debris and unidentified matters (Table 1). Dominant preys were phytoplankton (82.53%) and this was followed by plant-like matter (7.34%), debris (4.86%), copepod fragment (2.69%), algae (0.92%), unidentified food items (0.77%) and zooplankton (0.54%).
Monthly variation of diet: Highest frequency of occurrence of phytoplankton was observed throughout the year (Table 1). The highest percentage frequency of occurrence (Fpi) of phytoplankton (97.13%) was found in July. Nitzschia sp. was the most frequent phytoplankton found in the stomach content. The presence of Nitzschia sp. was continuous throughout the year with two major peaks in March (76.03%) and November (65.75%), respectively. Plant-like matter was observed in every month of sampling except in May (Table 1). Among all sampling months, the highest percentage of occurrence (Fpi) was seen in October (46.77%). The highest percentage occurrence of 14.65% and 14.01%, respectively for both debris and fragments of copepod were recorded in December. For algae the highest percentage frequency of occurrence (Fpi) was in January (9.59%) and for unidentified food items was seen in June (3.31%). The occurrence of zooplankton was recorded for three months that include January (4.11%), February (0.56%) and April (1.82%).
|| Monthly frequency occurrence (F pi) of food items
in the gut of larval fishes of Clupeidae from the estuary of Pendas river,
The food items are composed of seven major groups viz. phytoplankton, zooplankton,
copepod fragment, algae, plant-like matter, debris and unidentified food items.
According to the table ranked by frequency of occurrence (Fpi), the
dominant food item in the stomach is phytoplankton. Phytoplankton was observed
in the stomach of fish larvae in every month around the year. Hunter
and Thomas (1972) stated that phytoplankton is probably important for only
about the first week of feeding regime and a decline in percentage of phytoplankton
in the gut of anchovy larvae is seen with an increase in size (Arthur,
1976) Phytoplankton is likely higher quality food for zooplankton and macro
invertebrates as well as to the fish larvae. In this study, phytoplankton was
represented by Nitzschia sp, Dactylococcopis fascicularis and
Biddulphia sinensis and they were the common items in the stomachs of
the specimens. Nitzschia sp were present in the diet at highest frequencies
of (37.53%). The results are in contrast with those of Hunter
and Thomas (1972). According to Hunter and Thomas (1972),
the main item for phytoplankton in the stomach content was Gymnodium splendens.
Plant-like matter (7.34%) is the second in ranking of percentage frequency of
occurrence (Fpi), which meant it is the second, most important food
items consumed by the fish larvae from family Clupeidae. Zooplankton represented
by copepod, cladocera, rotifer, larvacean (appendicular), larval stage of Crustacea
(nauplii), siphonophores and thaliacian (tunicate). Zooplankton such as copepod
nauplii were not a significant item in the diet of the fish larvae analyzed
in this study, although they were significantly dominant in the planktonic environment.
This contrasts with the composition of the diet of the majority of marine fish
larvae which consume copepod nauplii at different frequencies, because of their
great abundance in the environment as well as their appropriate size as food
for fish larvae (Arthur 1976, Turner
1984, Watson and Davis, 1989). Pepin
and Penney (2000) observed in their study that larval predation pressure
on the zooplankton community was very low, but found that larval fish gradually
shifted their diet to larger prey items, thus limiting competition.
Robichaud-LeBlanc et al. (1997) stated that
different fishes consume different types of food and feeding habits of fishes
varies from season to season. Variations in the dominant food items found in
the stomachs of the fish larvae were more likely due to the temporal and spatial
availability of the prey in the particular water mass. In this study, the samples
of fish larvae were collected from estuary of Sg Pendas that have good distribution
of phytoplankton. Based on the results, foraging mainly on phytoplankton may
be one of the feeding strategies by the fish larvae from family Clupeidae. The
inability to swim swiftly may have restricted them to catch bigger and moving
prey such as the zooplankton.
The present study on food and feeding habits of fish larvae from family Clupeidae in the estuary of Pendas River, Gelang Patah, Johor indicates that clupeid larvae are herbivorous as the results showed that more than 80% of the food item was phytoplankton.
This study is part of a PhD research program funded by the Ministry of Science, Technology and Innovation (MOSTI), Malaysia (grant no. 05-01-04-SF0613). The authors would like to thank TWOWS (Third World Organization for Women Science) for providing a fellowship for PhD study.
1: Ara, R., A. Arshad, N. Amrullah, S.M.N. Amin, S.K. Daud, A.A. Nor Azwady and A.G. Mazlan, 2009. Feeding habits and temporal variation of diet composition of fish larvae (Osteichthyes: Sparidae) in the Sungai Pulai seagrass bed, Johore, Peninsular Malaysia. J. Biol. Sci., 9: 445-451.
CrossRef | Direct Link |
2: Ara, R., A. Arshad, S.M.N. Amin, S.K. Daud, J.S. Bujang and M.A. Ghaffar, 2010. Feeding habits of larval fishes of the family Gobiidae (Actinopterygii: Perciformes) in seagrass beds of Sungai Pulai estuary, Johor Strait, Malaysia. Coastal Mar. Sci., 34: 123-128.
Direct Link |
3: Ara, R., A. Arshad, S.M.N. Amin, S.K. Daud and M.A. Ghaffar, 2011. Environment and diversity of ichthyoplankton in the seagrass beds of sungai pulai estuary, Johor, Peninsular Malaysia. J. Food Agric. Environ., 9: 733-738.
Direct Link |
4: Arshad, A., R. Ara, S.M.N. Amin, M. Effendi, C.C. Zaidi and A.G. Mazlan, 2011. Influence of environmental parameters on shrimp post-larvae in the Sungai Pulai seagrass beds of Johor Strait, Peninsular Malaysia. Sci. Res. Essays, 6: 5501-5506.
Direct Link |
5: Arthur, D.K., 1976. Food and feeding of larvae of three fishes occurring in the California Current: Sardinops sagax, Engraulix mordax and Trachurus symmnetricus. Fish. Bull., 74: 517-530.
6: Bhuiyan, A.S., S. Afroz and T. Zaman, 2006. Food and feeding habits of the juvenile and adult snakehead, Channa punctatus (Bloch). J. Life Earth Sci., 1: 53-54.
7: Bhuiyan, A.S. and M.N. Islam, 1988. Seasonal variation in the percentage composition of the food of Xenentodon Cancila (Hamilton). Univ. J. Zool. Rajshahi Univ., 7: 33-36.
8: Blaber, S.J.M., M.J. Farmer, D.A. Milton, J. Pang, O. Boon-Teck and P. Wong, 1997. The ichthyoplankton of selected estuaries in Sarawak and Sabah: Composition, distribution and habitat affinities. Estuarine Coastal Shelf Sci., 45: 197-208.
CrossRef | Direct Link |
9: Chrisafi, P., P. Kaspiris and G. Katselis, 2007. Feeding habits of sand smelt (Atherina boyeri, Risso 1810) in Tichonis Lake (Western Greece). J. Applied Ichthyol., 23: 209-214.
10: Dadzie, S., F. Abou-Seedo and E. Al-Qattar, 2000. The food and feeding habits of the silver pomfret, Pampus argenteus (Euphrasen), in Kuwait waters. J. Applied Ichthyol., 16: 61-67.
CrossRef | Direct Link |
11: Chua, T.E. and J.A. Mathias, 1978. Coastal Resources of West Sabah: An Investigation into the Impact of Oil Spill. Penerbit Universiti Sains Malaysia, Malaysia, pp: 101-106
12: Hunter, J.R. and G.L. Thomas, 1972. Effect of Prey Distribution and Density on the Searching and Feeding Behaviour of Larval Anchovy Engraulis Mordax Girard. In: The Early Life History of Fish, Blaxter, J.H.S. (Ed.). Springer-Verlag, Heidelberg, New York, pp: 559-574
13: Jardas, I., M. Santic, V. Nerlovic and A. Pallaoro, 2007. Diet composition of blackspotted smoot-hound, Mustelus punctulatus (Risso, 1826), in the eastern Adriatic Sea. J. Applied Ichthyol., 23: 279-281.
14: Pepin, P. and R. Penney, 2000. Feeding by a larval fish community: Impact on zooplankton. Mar. Ecol. Prog. Ser., 204: 199-212.
Direct Link |
15: Robichaud-LeBlanc, K.A., S.C. Courtenay and J.M. Hanson, 1997. Ontogenetic diet shifts in age-0 striped bass, Morone saxatilis, from the Miramichi River estuary, Gulf of St. Lawrence. Can. J. Zool., 75: 1300-1309.
16: Turner, J.T., 1984. The feeding ecology of some zooplankters that are important prey items of larval fish. NOAA Technical Report NMFS 7. U.S. Department of Commerce, National Oceanic and Atmospheric Administration National Marine Fisheries Service, USA.
17: Watson, W. and R.L. Davis Jr., 1989. Larval fish diets in shallow coastal waters off San Onofre, California. Fish. Bull. USA., 87: 569-591.
Direct Link |
18: Whitehead, P.J.P., 1985. FAO species catalogue Vol 1. Clupeoid fishes of the world (suborder Clupeioidei). An annotated and illustrated catalogue of the herring, sardines, pilchards, sprats, shads, anchovies and wolf-herring. Part 1 chirocentridae clupeidae and pristigaste. FAO Fisheries Synopsis, 125: 1-303.