Population Fluctuations of Brown Plant Hopper Nilaparvata lugens Stal. And White Backed Plant Hopper Sogatella furcifera Horvath on Rice
Population fluctuation of Brown Plant Hopper (BPH) and White Backed Plant Hopper (WBPH) were studied in Myanmar for two seasons (rainy and summer). Experiments was conducted on a 5 ha rainfed unsprayed field and done in 5 experimental units with an area of 100x100 m. BPH and WBPH were counted from 30 rice hills out of 2000 hills randomly. Relative humidity, temperature, rainfall were also recorded. Population fluctuation study revealed that BPH population was high at 64 and 74 days after transplanting (in Mid September 2007) associated with heavy rainfall, high temperature and high humidity. The BPH population was lowest (in mid week October 2007) suggesting that low rainfall and low humidity were at least partially responsible for the decrease population of BPH. The WBPH was being passed thorough the same weather regime as BPH. When the rainfall decreased or trend to stop the population began to build up reach its peak. This trend of population fluctuation is not directly related with rainfall, but rainfall could be in influencing the physiology of rice plant. This can be seen in the correlation and regression analysis. The fluctuation of plant hopper were correlated with temperature and showed higher correlation with rainfall patterns during the first cropping season. Second cropping season coincide with dry season, there was no rainfall and hopper population was observed to be correlated to temperature and relative humidity. Thus temperature, rainfall and relative humidity were observed to influence plant hopper population during the two different rice growing seasons.
Received: April 16, 2010;
Accepted: May 26, 2010;
Published: July 22, 2010
Pest and disease problems are major constraints for increasing rice production.
Insect pests including plant hoppers can cause serious damage to rice crop.
Brown Plant Hopper (BPH) Nilaparvata lugens Stal. And White Backed Plant
Hopper (WBPH) Sogatella furcifera Horvath have become serious threat
to rice production throughout South and South East Asia since the early 1970s.
Lost of crop yield due to these pests is estimated at between 10 to 30%. Serious
damage usually occurs during the early stages of plant growth with symptoms
of hopper burns due to intensive sucking by the insect (Dale,
1994). The BPH is also a vector in transmitting virus diseases such as grassy
stunt, ragged stunt and wilted stunt (Hibino, 1979).
Dyck and Thomas (1979) observed serious outbreaks of
WBPH in West Malaysia following the outbreak of BPH and in Myanmar, the first
outbreak was recorded in 1981 and since then it has been recognized as one of
the most serious and widespread pest of rice.
An insect population always fluctuates according to the dynamic condition of
its environment. Both physical (abiotic) and biotic factors are believed to
be the factors responsible for the change in a population. Canedo
(1980) stated four components that influenced BPH populations, namely extension
of irrigation which allowed double cropping of rice, short duration photoperiod
intensive rice varieties used, more nitrogenous fertilizers applied and 4 intensive
insecticides application There is evidence that these factors can cause plant
hopper outbreaks. Andrewwartha and Birch (1954) noted
four components of the environment that could influence insect populations
viz. weather condition, food, other insects or organisms causing diseases and
a place in which to live. Climatic factors such as temperature and rainfall
and relative humidity have known to greatly influence the insect population
change (Muhamad and Chung, 1993; Way
and Heong, 1994; Zhu, 1999; Heong
et al., 2007; Siswanto et al., 2008).
Knowledge of the seasonal abundance and trends in the population build up of
a pest has become important for its effective control schedules (Siswanto
et al., 2008). Food supply, improved irrigation and water standing in
the rice field are conducive to the growth of BPH and WBPH populations. Lowland
rice fields have greater pest problems than upland fields. The change from upland
to lowland culture caused the development of BPH and WBPH as the main rice pests
and wet cultivation of rice leads to more BPH and WBPH (IRRI,
1979). Therefore, the objectives of this study were to investigate the population
fluctuations of BPH and WBPH in the field and also to determine the influence
of ecological factors to their populations in the field.
MATERIALS AND METHODS
The experiments were carried out in Myanmar for two seasons within 2007 to 2008 during the rainy rice season and summer rice season successively. Unsprayed rice field was chosen for the experiments. The population study was conducted on a 5 ha rainfed rice field. The experiments were conducted in 5 experimental units with an area of 100x100 m (1000 m2). The plant spacing being applied in each experimental plot was 20x15 cm to achieve 2000 rice hills. The data of plant hoppers (BPH and WBPH) were counted from 30 rice hills out of 2000 hills randomly. For the practical way of counting the insect, the plant hoppers were recorded from three rice plants per hill. The number of plant hopper, relative humidity, temperature and rainfall were recorded. Data included the maximum and minimum temperature and rainfall. Paired t-test at 5% level of probability was employed to compare the population density between two seasons. Regression analysis was done to determine the influence of abiotic factors on the insect population fluctuation. The population abundance of BPH and WBPH in relation to plant density, average temperature, rainfall and relative humidity was recorded in the rice field in 2007 and 2008 rice-growing seasons.
RESULTS AND DISCUSSION
Table 1 shows the coefficient correlation (R) between BPH
and WBPH populations and environmental factors during rainy and dry seasons.
The month of July-August 2007 was the middle of the rainy season. However, the
month of September 2007 was post monsoon period in Myanmar. Thus during the
July-September 2007 of the rainy season the BPH build up coincided with maximum
tillering stage of rice plants in the field. Rainfall had reached its maximum
and with the highest in July-August.
||Coefficient correlation(r) between BPH and WBPH populations
and environmental factors during rainy (2007) and dry (2008) seasons
|*Significant at p = 0.05, **Highly significant at p = 0.01,
|| Coefficient correlation(r) between number of tillers with
environmental factors during rainy (2007) and dry (2008) seasons
|*Significant at p = 0.05, **Highly significant at p = 0.01.
However, it gradually declined in September. In October, the rainy had eventually
ceased. Intermittent rainfall occurred continuously causing the BPH population
to fluctuate until 4th October and reaching a peaks between July and September.
However, as the tillering decreased during the period, the Early Panicle Initiation
(EPI) stage of the rice plant was initiated. However, the population of BPH
had declined. From November, the BPH population was clearly noted to increase.
This was followed by another population peak and thereafter a decline. The whole
of January to April 2008 (dry season) showed the decline of the BPH population.
Table 1 also shows that there was a significant correlation between populations of both plant hoppers with a biotic factor, except for during the dry season. There were positive correlations between BPH and WBPH populations with rainfall, temperature and relative humidity with the exception of negatively correlation with rainfall during the dry season and with temperature in the rainy season. BPH population was significantly correlated with rainfall (R = 0.918) and relative humidity (R = 0.725) but significantly and negatively correlated with temperature (R = -0.868) during the rainy season. The average temperature and relative humidity were 23.57-27.64°C and 58.59-9.28%, respectively. WBPH population was significantly correlated with rainfall (R = 0.873) and relative humidity (R = 0.717) but significantly and negatively correlated with temperature (R = - 0.843) during the rainy season. Both plant hopper populations were positively correlated with temperature in the dry season. However, BPH and WBPH populations were negatively correlated with rainfall in the dry season.
Similar result of relationship between number of rice tillers with abiotic
factor (Table 2) as significant correlation between numbers
of tillers with abiotic factor except for during dry the season in 2008. However,
there were positive correlations between number of tillers with both relative
humidity and rainfall. On the other hand, the number of tillers were negatively
correlated (R = -0.812) with temperature during the rainy season as opposed
to the dry season. Tiller numbers were significantly correlated with rainfall
(R = 0.940) and relative humidity (R = 0.640) during rainy season. While number
of tillers per hill of rice plants appeared to show similar trend for BPH and
WBPH populations in the rice fields, seven maximum numbers of tillers were recorded.
Beginning in July, the highest number of tillers was recorded. During the middle
of raining and post monsoon season (August-September), there was two peaks of
tillers numbers recorded. This was followed by number maximum tillering stages,
until the number of tillers/plant was decreased up to October.
|| Stepwise regression of plant hoppers against rainfall and
temperature during rainy (2007) and dry (2008) seasons
||Stepwise regression for number of tillers against environmental
factors during rainy (2007) and dry (2008) seasons
However, between December and January was dry season, 2 peaks of tillers/plant
were recorded between February to March. During the dry season, the number of
tillers/plant was gradually declined.
The stepwise regression analysis constructed to investigate which abiotic factors contributed the most to the variation of plant hopper population (Table 3). Step wise regression analysis showed that rainfall significantly contributed more than 80 (84.2%) to the variation of BPH and 75 (76.1%) to the variation of WBPH population during the raining season. Stepwise regression analysis also showed that temperature significantly contributed only 6.3 and 7.1% to the variation of BPH and WBPH populations during rainy season. Relative humidity did not significantly contribute to the variations observed. During the dry season, temperature significantly contributed about 75 (74.8%) to the variation of BPH population. Relative humidity contributed only 21.9 and 12.3% the variation of BPH and WBPH population, respectively. Rainfall did not significantly contribute to the population variations. The population was significantly and positively correlated with average temperature and relative humidity. However, stepwise regression analysis showed that contribution of lower population of WBPH in the dry season was average temperature. Average temperature significantly contributed more than 80 (85.5%) to the variation in WBPH population. The relative humidity contributed only (12.3%) to the variation in population. Rainfall did not significantly contribute to the population variation.
Table 4 shows that during the rainy season, rainfall significantly
contributed to 88.4% in variation of number of tillers. Temperature and relative
humidity did not significantly contribute to the plant hopper population. During
dry season, relative humidity significantly contributed 75.8% to the variation
in number of tillers while temperature contributed 22.3% to the variation in
the number of tillers. However, rainfall did not significantly contribute to
the variation in number of tillers. The findings of this study showed that the
increase population of plant hoppers could be associated with the heavy rainfall
(32.86 mm), high temperature (28°C) and high relative humidity (92%) at
the time of sampling. However, the results also suggested that low rainfall
and low humidity to be partially responsible for decrease populations of BPH
The step wise regression analysis was also constructed to investigate which abiotic factors contributed to the most number of tillers (Table 4). The number of tiller increments appeared to coincide with maximum tillering stage during the raining season. The rainfall pattern was high in the first week of July, August and September. However, the rainfall started to decrease in October and subsequently to January and April 2008. Therefore, rainfall was directly correlated with the trend of number of tillers increment. This can be observed by looking at the results of correlation and regression analysis of these parameters which showed rainfall to be significantly and positively correlated with number of tillers per plant. Meanwhile correlation analysis between numbers of tillers per plant with temperature had shown negative correlation during the rainy season. This study indicated that rainfall and relative humidity both influenced the number of tillers per plant as well as plant hopper populations. Regression analysis however, suggested that rainfall was (R2 = 0.884) contributed to the increment of number of tillers (Table 4).
Figure 1a and b again showed that the population
abundance of WBPH in relation to tiller numbers, average temperature, rainfall
and relative humidity. Two population peaks of WBPH were noted in July and September.
The population of WBPH fluctuated until 11th October 2007. When the tillering
stage ended, Early Panicle Initiation (EPI) stage of rice was initiated followed
by the decline in October. Through out this period, the average temperature
and relative humidity were similar as previously discussed.
||(a, b) Population abundance of BPH and WBPH on rice plant
in relation to number of tillers per hill, rainfall, temperature and relative
humidity at Hmawbe rice field within July, 2007-April, 2008
In the analysis of correlation (Table 1), WBPH population
fluctuations were significantly correlated with rainfall (R = 0.873) and relative
humidity (R = 0.717) but significantly and negatively correlated with temperature
(R = -0.843).
During the dry season and after the cessation of rainfall, heading and flowering occurred followed by grain filling and maturity of the rice plants. During the middle of this period, rainfall was relatively very low with rainfall recorded in December to February. During this rice maturity period and harvesting season, BPH began to build up and populations peaked in December and January 2008. The BPH population was significantly correlated with temperature (R = 0.813) and relative humidity (R = 0.865) and identified as factors significantly contributing to the build up of the plant hopper populations.
During the early part of the dry season, the temperature and relative humidity also fluctuated slightly. During the post flowering-maturity season, WBPH population slightly dropped and increased again and was positively correlated with average temperature. However, stepwise regression analysis showed that the contribution to plant hopper population was due to temperature, significantly contributing to more than (70%) in the variation of BPH population. Relative humidity contributed to 21.9% in the variation of BPH population during the dry season.
The stepwise regression analysis results showed that average temperature significantly
contributed to the variation in BPH (R2 = 0.748) and WBPH (R2
= 0.855) populations during the dry season. The actual pattern of population
fluctuation appeared very distinct during the first 4-5 weeks after transplanting.
The macropterous adults that emigrated from the outside field became dominant
while some brachypterous adults had originated from nursery beds. Three distinct
peaks could be observed, each between 3 to 4 week intervals corresponding to
the period of approximately one BPH generation recorded in South East Asia countries.
In rice fields within these regions the plant hopper populations were known
in many cases to show rather discrete generation cycle (Sawada
et al., 1993). This could be the most probable cause, or partially
explained by the preference of macropterous immigrants to young rice plants.
Predators are the most important natural enemies of plant hoppers and together
with parasitoids and insect pathogens keep their populations down. Observations
in rice field show a high population of predators especially spiders which are
carnivorous arthropods. Research had shown that spiders in rice fields can play
an important role as predators in reducing the densities of plant hoppers and
leaf hoppers (Holt et al., 1987; Tanaka,
1989; Mu et al., 2000). Among the predators
identified are several species of spiders such as Lycosa sp., Oxyopos
and a mirid, Cyrtorhinus sp. which attacked eggs and young nymphs that
were predominantly found in abundance (Sawada et al.,
Muhamad and Chung (1993) reported that H. theivora
population fluctuations appeared to be influenced by rainfall and by the numbers
of available pods as shown by positive correlation between mired numbers, rainfall
and numbers of pods. This study concurs with the results of the present investigation
where plant hopper populations were strongly and positively correlated with
rainfall. Patnaik et al. (1985) reported that
a well-distributed heavy precipitation could increase the mesospheric humidity
to more than 85%. Statistical analysis of data had shown a positive and significant
correlation between midge incidence and relative humidity. This was in agreement
with the present investigations which recorded higher population of BPH due
to heavy rainfall, high relative humidity and high temperature at the time of
sampling. The BPH population was found to be strongly and positively correlated
win rainfall and relative humidity. The results of this study were, however,
inconsistent with the findings of Isichaikul and Ichikawa
(1993) that microhabitat of nymphs and the adults of BPH were found to be
on the lower parts of the rice plants. It was proven in this study that relative
humidity was an important environmental factor to determine the microhabitat
at of the nymphs of BPH as they prefer very humid conditions of more than 90%
The number of WBPH immigrants were highest on rice plants 17 to 30 Days After
Transplanting (DAT) suggesting that they prefer to settle or remain more on
rice plants at the tillering stage of approximately 20-30 DAT. Plots that were
fertilized early attracted more BPH. Reproduction of WBPH immigrants was enhanced
by early fertilization in rice fields. The WBPH was found to be dominant species
in the early season <60 DAT, while BPH was more dominant in the late season
>60 DAT (Zhu et al., 1993; Matsumura,
1996; Zhu, 1999). These findings differed from the
present investigation where BPH and WBPH populations were also shown to increase
at 64 days after transplanting.
The population abundance of BPH and WBPH in relation to plant density, average temperature, rainfall and relative humidity was recorded over two rice growing seasons in Myanmar. During the rainy season, BPH population was found to build to coincide with increase in plant tillers in the rice field. Two peaks of BPH populations were noted in July and August. The population peaks recorded for WBPH were similar with BPH.
During the period of rice maturity and harvesting season, BPH population began to build up and peaking in December 2007 and January 2008. The population subsequently declined in January. It began to increase and recorded 3 population peaks in February, March and April 2008 during the dry season. WBPH which passed through a similar weather regime showed a more or less similar trend to BPH. The plant hopper population abundance was strongly associated with standing plant population in the rice field. As with the plant hopper populations in the field, increase in BPH numbers per tiller began in the field and reaching two peaks during the rainy season. The BPH number per tiller also increased with two peaks in December 2007 and January 2008. The WBPH numbers per tiller showed fluctuations that were similar to those observed for BPH in rice field.
This study was supported by funds from Third World Organization for Women in Science (TWOWS) to Ms San San Win.
Andrewwartha, H.G. and L.C. Birch, 1954. The Distribution and Abundance of Animals. University of Chicago Press, Chicago, IL., USA.
Canedo, F.M., 1980. Change agents perceived credibility and their influence in the innovation decision process of development program. Ph.D. Theses, University of the Philippines, Los Banos, Laguna, Philippines.
Dale, D., 1994. Insect Pest of Rice Plant-Their Biology and Ecology. In: Biology and Management of Rice Insects, Heinrichs, E.A. (Ed.). Wiley, New York, ISBN: 0-70-21814-2, pp: 363-485.
Dyck, V.A. and B. Thomas, 1979. The Brown Planthopper Problem. In: Brown Planthopper: Threat to Rice Production in Asia, International Rice Research Institute (Ed.). IRRI, Los Banos, Philippines, pp: 3-17.
Heong, K.L., A. Manza, J. Catindig, S. Villareal and T. Jacobsen, 2007. Changes in pesticide use and arthropod biodiversity in the IRRI research farm. Outlooks Pest Manage., 18: 229-233.
Direct Link |
Hibino, H., 1979. Rice ragged stunt, a new virus disease occurring in tropical Asia. Rev. Plant Prot. Res., 12: 98-110.
Holt, J., A.G. Cook, T.J. Perfect and G.A. Norton, 1987. Simulation analysis of brown planthopper (Nilarparvata lugens) population dynamic on rice in the Philippines. J. Applied Ecol., 24: 87-102.
Direct Link |
IRRI, 1979. Brown Plant Hopper: Threat to Rice Production in Asia. In: Brown Plant Hopper: Threat to Rice Production in Asia, International Rice Research Institute (Ed.). IRRI, Los Banos, Philippines, pp: 369.
Isichaikul, S. and T. Ichikawa, 1993. Relative humidity as an environmental factor determining the microhabitat of the nymphs of the rice brown hopper, N. lugens (Stal) (Homoptera: Delphicidae). Res. Popul. Ecol., 35: 361-373.
CrossRef | Direct Link |
Matsumura, M., 1996. Population dynamics of the white-backed planthopper; S. furcifera (Hemiptera: Delphacidae) with special reference to the relation ship between its population growth and growth stages of rice plant. Res. Popul. Ecol., 36: 19-25.
Mu, K., S.S. Win, M. Thwin and R.P. Kaushik, 2000. Studies on the resistance to BPH N. lugens, WBPH S. furcifera (Horvath) and biotypes of BPH by varietal reaction. Paper Presented at Burma Research Congress, Yangon.
Muhamad, R. and G.T. Chung, 1993. The relationship between population fluctuations of Helopeltis theivora Waterhouse, availability of cocoa pods and rainfall pattern. Pertanika J. Trop. Agric. Sci., 16: 81-86.
Direct Link |
Patnaik, N.C., J.M. Satpathy, R. Muhamad and C.G. Fee, 1985. Influence of biotic and abiotic and factors on the distribution and abundance of rice galmidge, (Rscolia oryzae) (Wood-Mason) (Diptera: Cecidomyiidae). J. Ent. Res., 9: 122-128.
Sawada, H., A. Kusmayadi, S.W.G. Subroto, E. Suwardiwijaya and Mustaghfirin, 1993. Comparative analysis of population characteristic of the brown planthopper, Nilaparvata lugens Stal, between wet and dry rice cropping seasons in West Java, Indonesia. Res. Popul. Ecol., 35: 113-137.
Direct Link |
Siswanto, M. Rita, O. Dzolkhifli and K. Elna, 2008. Population fluctuation of Helopeltis antoni Signoret on Cashew Anacarcium occidentalle L., in Java Indonesia. Pertanika J. Trop. Agric. Sci., 31: 191-196.
Tanaka, K., 1989. Movement of spiders in arableland. Plant Prot., 43: 34-39.
Way, M.J. and K.L. Heong, 1994. The role of biodiversity in the dynamics and management of insect pests of tropical irrigated rice-a review. Bull. Entomol. Res., 84: 567-587.
Direct Link |
Zhu, Z.R., 1999. Population ecology and management strategy of the white backed planthoppers S. furcifera (Horvath) in subtropical rice. Ph.D. Thesis, Nanjing Agricultural University China.
Zhu, Z.R., J.A. Cheng and X. Chen, 1993. Host preference and suitability of Anagrus nilaparvatae. Acta Entomol. Sinica, 36: 430-437.