Subscribe Now Subscribe Today
Research Article

Optical Microscope Based on Multispectral Imaging Applied to Plasmodium Diagnosis

Jeremie T. Zoueu, Georges L. Loum, T. Cisse Haba, Mikkel Brydegaard and Herve Menan
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

We have adapted an optical transmission microscope to multispectral imaging in order to diagnose Plasmodium falciparum without using fluorescent labels for parasites as is the case for the thick and thin blood smears. We show how a set of monochromatic Light Emitting Diodes (LEDs) has provided an efficient contrast and spatial resolution to identify the parasites inside the erythrocytes. The obtained images have been processed using MATLAB software to improve the contrast. This method is a promising alternative for malaria diagnosis.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Jeremie T. Zoueu, Georges L. Loum, T. Cisse Haba, Mikkel Brydegaard and Herve Menan, 2008. Optical Microscope Based on Multispectral Imaging Applied to Plasmodium Diagnosis. Journal of Applied Sciences, 8: 2711-2717.

DOI: 10.3923/jas.2008.2711.2717


Banister, L.H., J.M. Hopkins, R.E. Fowler, S. Krishma and G.H. Mitchell, 2000. A brief illustrated guide to the ultra structure of Plasmodium falciparum asexual blood stages. Parasitol. Today, 16: 427-433.
Direct Link  |  

Belisle, J.M., S. Costantino, M.L. Leimanis, M.J. Bellemare, D.S. Bohle, E. Georges and P.W. Wiseman, 2007. Sensitive detection of malaria infection by third harmonic generation imaging. Biophys. J. Biofast, doi:10.1529/biophysj.107.125443

Borisova, E.G., P.P. Troyanova and L.A. Avramov, 2007. Fluorescence spectroscopy for early detection and differentiation of cutaneous pigmented lesions Optoelect. Adv. Mater. Rapid Commun., 1: 383-393.

Bremard, C., J.J. Girerd, P. Kowalewski, J.C. Merlin and S. Moreau, 1993. Spectroscopic investigation of malaria pigment. Applied Spectroscopy, 47: 1837-1842.
Direct Link  |  

Brydegaard, M., Z. Guan and S. Svanberg, 2007. Optics and laser applications in medicine and environmental monitoring for sustainable development. Proceedings of the Optolasermed Conference, November 19-24, 2007, Accra, Ghana, pp: 91-92.

Frosh, T., S. Koncarevic, L. Zedler, M. Schmitt, K. Schenzel, K. Becker and J. Popp, 2007. In situ localization and structural analysis of the malaria pigment hemozoin. J. Phys. Chem., B, 111: 11047-11056.

Fung, Y.C., W.C. Tsang and P. Patitucci, 1981. High-resolution data on the geometry of red blood cells. Biorheology, 18: 369-385.

Kaplan, P.D., A.D. Dinsmore and A.G. Yodh, 1994. Diffuse-transmission spectroscopy: A structural probe of opaque colloidal mixtures. Phys. Rev. E, 50: 4827-4835.

Liu, F., K.M. Yoo and R.R. Alfano, 1994. Transmitted photon intensity through biological tissues within various time windows. Opt. Lett., 19: 740-742.
Direct Link  |  

Nyyssonen, D., 1977. Linewidth measurement with an optical microscope: The effect of operating conditions on the image profile. Applied Opt., 16: 2223-2230.
Direct Link  |  

Ong, C.W., Z.X. Shen, K.K.H. Ang, U.A.K. Kara and S.H. Tang, 2002. Raman microspectroscopy of normal erythrocytes and Plasmodium berghei-infected erythrocytes. Applied Spectroscopy, 56: 1126-1131.
Direct Link  |  

Sheppard, C.R.J. and M. Gu, 1986. Scanning methods in optical microscopy. Endeavour, 10: 17-19.

Streibl, N., 1985. Three-dimensional imaging by a microscope. J. Opt. Soc. Am. A, 2: 121-127.
Direct Link  |  

Webster, G.T., L. Tilley, S. Deed, D. McNaughton and B.R. Wood, 2008. Resonance raman spectroscopy can detect structural changes in haemozoin (malaria pigment) following incubation with chloroquine in infected erythrocytes. FEBS Lett., 582: 1087-1092.
Direct Link  |  

Wood, B.R., S.J. Langford, B.M. Cooke, F.K. Glenister, J. Lim and D. McNaughton, 2003. Raman imaging of hemoglobin within the food vacuole of Plasmodium trophozoites. FEBS Lett., 554: 247-252.
Direct Link  |  

©  2020 Science Alert. All Rights Reserved