C.N. Fokunang
Structural Biology Unite, The University of Reading, UK
K.A. Watson
Structural Biology Unite, The University of Reading, UK
A. Purvis
Structural Biology Unite, The University of Reading, UK
E.A. Tembe- Fokunang
Pfizer Global Research and Development, Sandwich Laboratories, UK
ABSTRACT
The aim of this study was to develop an in-house high throughput method of crystallization and structure determination of lysozyme enzyme as model in structure-based drug design studies. Lysozyme stock solution at 50 mg mL-1 from hen egg white was crystallized using the vapour diffusion techniques in buffer A [0.1 M Na acetate/acetic acid pH 4.8, 0.2% Na azide (w/v), 1.1 M NaCl] and buffer B [0.1 M Na acetate/acetic acid pH 4.8, 0.02% Na azide (w/v), 25% ethylene glycol (w/v), 1.7 m M NaCl]. Two 24 well plates in duplicates were used for each buffer preparations and incubated at 4°C. High quality crystals between 0.2-0.5 mm sizes, were produced in buffer A plate well after days 2 of incubation and none in buffer B. The crystal data collection and refinement showed a high resolution of the outer shell of 2.28-1.92 Å, completeness of 98.7%, space group P43212 and mosaicity of 0.415.
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How to cite this article
C.N. Fokunang, K.A. Watson, A. Purvis and E.A. Tembe- Fokunang, 2005. Crystallization and Structural Studies of Lysozyme from Hen Egg White, Using Vapour Diffusion Techniques. Biotechnology, 4: 341-346.
DOI: 10.3923/biotech.2005.341.346
URL: https://scialert.net/abstract/?doi=biotech.2005.341.346
DOI: 10.3923/biotech.2005.341.346
URL: https://scialert.net/abstract/?doi=biotech.2005.341.346
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