Subscribe Now Subscribe Today
Research Article

Characterization of Chemical Constituent and Evaluation of Antioxidant, Cytotoxicity, Potential of Selenicereus hamatus Crude Extract

Isaac John Umaru, Maryam Ahmed Usman and Abdulrashid Mohammed
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Background and Objective: The plant Selenicereus hamatus is a distinct species distantly related to the Grandiflora-complex, a species of Cactaceae and produces one of the largest flowers in the family of Cactaceae. The main aim of this study was to find out the antioxidant, cytotoxicity of the crude extract and to isolate, characterization of a chemical constituent of Selenicereus hamatus. Materials and Methods: The antioxidant of the methanol extract of Selenicereus hamatus was evaluated of its potential using DPPH and the cytotoxicity was evaluated using Brine shrimps (Artemia salina). Extraction, isolation and characterization of the isolated compound were done using methanol, CC, UV, TLC, GC-MS, NMR and FTIR. Results: The maximum potential of antioxidant was obtained from the isolated compound from the crude extract of Selenicereus hamatus of 99 μg mL–1, followed by the methanol crude extract of 234 μg mL–1. Cytotoxicity was obtained to mild in crude extract (58.71 μg mL–1) and high in the isolated compound of 16.52 μg mL–1 Conclusion: The study showed that the crude extract from Selenicereus hamatus has Neophytadiene and significant antioxidant and cytotoxicity activity which can be a potential agent to curtail the menace caused by pathogens and many more diseases like cancer. The pure compound was isolated for the first time in this plant extract as Neophytadiene.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Isaac John Umaru, Maryam Ahmed Usman and Abdulrashid Mohammed, 2020. Characterization of Chemical Constituent and Evaluation of Antioxidant, Cytotoxicity, Potential of Selenicereus hamatus Crude Extract. Asian Journal of Biochemistry, 15: 50-59.

DOI: 10.3923/ajb.2020.50.59


1:  Korotkova, N., T. Borsch, and S. Arias, 2017. A phylogenetic framework for the Hylocereeae (Cactaceae) and implications for the circumscription of the genera. Phytotaxa, 327: 1-46.
CrossRef  |  Direct Link  |  

2:  Nyffeler, R. and U. Eggli, 2010. A farewell to dated ideas and concepts: molecular phylogenetics and a revised suprageneric classification of the family Cactaceae. Schumannia, 6: 109-149.
Direct Link  |  

3:  Schlumpberger, B.O. and S.S. Renner, 2012. Molecular phylogenetics of Echinopsis (Cactaceae): Polyphyly at all levels and convergent evolution of pollination modes and growth forms. Am. J. Bot., 99: 1335-1349.
CrossRef  |  Direct Link  |  

4:  Zou, D.M., M. Brewer, F. Garcia, J.M. Feugang and J. Wang et al., 2005. Cactus pear: A natural product in cancer chemoprevention. Nutr. J., 10.1186/1475-2891-4-25

5:  Singh, G., 2004. An overview of cactus pear research and development in India. Acta Hortic., 728: 43-50.
CrossRef  |  Direct Link  |  

6:  Del-Valle, V., P. Hernandez-Munoz, A. Guarda and M.J. Galotto, 2005. Development of a cactus-mucilage edible coating (Opuntia ficus indica) and its application to extend strawberry (Fragaria ananassa) shelf life. Food Chem., 91: 751-756.
CrossRef  |  

7:  Castro-Enríquez, D.D., Beatriz Montaño-Leyva, C.L. Del Toro-Sánchez, J.E. Juárez-Onofre and E. Carvajal-Millán et al., 2020. Effect of ultrafiltration of pitaya extract (Stenocereus thurberi) on Its phytochemical content, antioxidant capacity, and UPLC-DAD-MS profile. Mol., 10.3390/molecules25020281

8:  Castellar, R., J.M. Obon, M. Alacid and J.A. Fernandez-Lopez, 2003. Color properties and stability of betacyanins from Opuntia fruits. J. Agric. Food Chem., 51: 2772-2776.
CrossRef  |  Direct Link  |  

9:  Britton, N.L. and J.N. Rose, 1990. The genus Cereus and its Allies in North America. Nabu Press, South Carolina, Pages: 70.

10:  Shetty, A.A., M.K. Rana and S.P. Preetham, 2012. Cactus: a medicinal food. J. Food Sci. Technol., 49: 530-536.
CrossRef  |  Direct Link  |  

11:  Young, I.S. and J.V. Woodside, 2001. Antioxidants in health and disease. J. Clin. Pathol., 54: 176-186.
CrossRef  |  PubMed  |  Direct Link  |  

12:  Anderson, K.M., P.M. Odell, P.W. Wilson and W.B. Kannel, 1991. Cardiovascular disease risk profiles. Am. Heart J., 121: 293-298.
CrossRef  |  Direct Link  |  

13:  Umaru, I.J., F.A. Badruddin and H.A. Umaru, 2019. Phytochemical screening of essential oils and antibacterial activity and antioxidant properties of Barringtonia asiatica (L) leaf extract. Biochem. Res. Int., 10.1155/2019/7143989

14:  Umaru, I.J., F.B. Ahmed, K.I. Umaru and A.O. Omolayo, 2020. Extraction and biological activity of Barringtonia asiatica stem-bark extracts on some selected fungi, bacteria's, cytotoxicity and antioxidant potentials. Ind. J. Pure App. Biosci., 8: 6-15.
CrossRef  |  Direct Link  |  

15:  Wakawa, H.Y. and B.A. Fasihuddin, 2017. Brine Shrimp Lethality Bioassay of Abrus Precatorius (Linn) Leaves and Root Extract. Int. J. Pharm. Pharma. Sci., 9: 179-181.
CrossRef  |  Direct Link  |  

16:  Umaru, I.J., F.A. Badruddin, Z.B. Assim and H.A. Umaru, 2018. Antibacterial and cytotoxic actions of chloroform crude extract of Leptadenia hastata (pers) decnee. Clin. Med. Biochem., 10.4172/2471-2663.1000139

17:  Umaru, I.J., F.A. Badruddin and H.A. Umaru, 2018. Antioxidant properties and antibacterial activities of Leptadenia hastata leaves extracts on Staphylococcus aureus. Designing Intellectual Prop. Int. J., 10.32474/DDIPIJ.2018.01.000122

18:  Umaru, I.J., F.B. Ahmed, M. Abdulrashid and M.A. Ahmed, 2020. Extraction, isolation, characterization of caryophyllene from Barringtonia asiatica stem-bark extracts and biological activity. Int. J. Phar. & Biomedi. Rese., 7: 1-15.
CrossRef  |  Direct Link  |  

19:  Umaru, I.J., F.A. Badruddin and H.A. Umaru, 2019. Extraction, isolation and characterization of new compound and anti-bacterial potentials of the chemical constituents compound from Leptadenia hastata leaf extract. Chemrxiv, Online.

20:  Umaru, I.J. and H.A. Umaru, 2019. Isolation and characterization of chemical constituents, cytotoxicity, antibacterial and antioxidant activity of the compounds from Leptadenia hastata roots extract. Am. J. Biochem. Biotechnol., 15: 251-269.
CrossRef  |  Direct Link  |  

21:  Fasihuddin, B.A., N.M.S.K. Nisa and Z.B. Assim, 2010. Chemical constituents and antiviral study of Goniothalamus velutinus. J. Fundam. Sci., 6: 73-76.
Direct Link  |  

22:  Umaru, I.J., F.B. Ahmad and H.A. Umaru, 2019. Extraction, elucidation, characterization and evaluation of antibacterial activity of four pure compound from Barringtonia racemosaleaf extract. World J. Pharm. Pharm. Sci., 8: 184-223.
CrossRef  |  Direct Link  |  

23:  Umaru, I.J., F.B. Ahmed, and K.I. Umaru, 2020. Cinnamic acid from Barringtonia asiatica stem-bark extract. Its cytotoxicity, antioxidant and bioactive potentials on some selected pathogens. Int. J. Recent Biotechnol., 7: 35-46.
CrossRef  |  

24:  Singh, N. and P.S. Rajini, 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem., 85: 611-616.
CrossRef  |  

25:  Moshi, M.J., D.F. Otieno and A. Weisheit, 2012. Ethnomedicine of the Kagera Region, North Western Tanzania. Part 3: Plants used in traditional medicine in Kikuku village, Muleba District. J. Ethnobiol. Ethnomed., Vol. 8. 10.1186/1746-4269-8-14

26:  Gerothanassis, I.P., A. Troganis, V. Exarchou, and K. Barbarossou, 2002. Nuclear magnetic resonance (NMR) spectroscopy: basic principles and phenomena, and their applications to chemistry, biology and medicine. Chem. Educ. Res. Pract., 3: 229-252.
Direct Link  |  

27:  Adnan, M., N.U. Chy, A.T.M.M. Kamal, M.O.K. Azad, A. Paulet al., 2019. Investigation of the biological activities and characterization of bioactive constituents of Ophiorrhiza rugosa var. prostrata (D. Don) & Mondal leaves through in vivo, in vitro, and in silico approaches. Mol., Vol. 24. 10.3390/molecules24071367

28:  Suriani, N.L., 2018. Bioactive substance use of leaf extract of piper caninum blume pressing for blas disease and increase production in rice. Int. J. Life Sci., 2: 42-50.
CrossRef  |  Direct Link  |  

29:  Li, R.W., D.N. Leach, S.P. Myers, G.J. Leach, G.D. Lin, D.J. Brushett and P.G. Waterman, 2004. Anti-inflammatory activity, cytotoxicity and active compounds of Tinospora smilacina Benth. Phytother. Res., 18: 78-83.
CrossRef  |  PubMed  |  Direct Link  |  

30:  Ogunlesi, M., W. Okiei, M. Ademoye and E.A. Osibote, 2010. Analysis of essential oil from the stem of Chansmanthera dependens. J. Nat. Prod., 3: 47-53.
Direct Link  |  

©  2021 Science Alert. All Rights Reserved