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Research Article
 

Characterization of Chemical Constituent and Evaluation of Antioxidant, Cytotoxicity, Potential of Selenicereus hamatus Crude Extract



Isaac John Umaru, Maryam Ahmed Usman and Abdulrashid Mohammed
 
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ABSTRACT

Background and Objective: The plant Selenicereus hamatus is a distinct species distantly related to the Grandiflora-complex, a species of Cactaceae and produces one of the largest flowers in the family of Cactaceae. The main aim of this study was to find out the antioxidant, cytotoxicity of the crude extract and to isolate, characterization of a chemical constituent of Selenicereus hamatus. Materials and Methods: The antioxidant of the methanol extract of Selenicereus hamatus was evaluated of its potential using DPPH and the cytotoxicity was evaluated using Brine shrimps (Artemia salina). Extraction, isolation and characterization of the isolated compound were done using methanol, CC, UV, TLC, GC-MS, NMR and FTIR. Results: The maximum potential of antioxidant was obtained from the isolated compound from the crude extract of Selenicereus hamatus of 99 μg mL–1, followed by the methanol crude extract of 234 μg mL–1. Cytotoxicity was obtained to mild in crude extract (58.71 μg mL–1) and high in the isolated compound of 16.52 μg mL–1 Conclusion: The study showed that the crude extract from Selenicereus hamatus has Neophytadiene and significant antioxidant and cytotoxicity activity which can be a potential agent to curtail the menace caused by pathogens and many more diseases like cancer. The pure compound was isolated for the first time in this plant extract as Neophytadiene.

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  How to cite this article:

Isaac John Umaru, Maryam Ahmed Usman and Abdulrashid Mohammed, 2020. Characterization of Chemical Constituent and Evaluation of Antioxidant, Cytotoxicity, Potential of Selenicereus hamatus Crude Extract. Asian Journal of Biochemistry, 15: 50-59.

DOI: 10.3923/ajb.2020.50.59

URL: https://scialert.net/abstract/?doi=ajb.2020.50.59
 

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