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Articles by Amer Jamil
Total Records ( 9 ) for Amer Jamil
  Amer Jamil , Lubna Bashir and Hamid Yaqoob
  The effect of black gram starch on the utilization of casein was also tested in albino rats using PER, digestibility coefficient, biological value and net protein utilization as parameters. Soaking reduced the levels of sugars, starch and oligosaccharides but starch digestibility was improved significantly. Cooking increased the level of sugars while starch and verbascose content was decreased. Starch digestibility was also improved on cooking. Stachyose and raffinose contents were increased when unsoaked seeds were cooked, but decreased when the seeds were soaked before cooking. Germination decreased starch and oligosaccharide contents thereby raising the level of the soluble sugars. Starch digestibility was increased appreciably on germination. On cooking, the black gram starch promoted growth similar to corn starch as no difference was noticed in the parameters studied in albino rats.
  Numaira Kaleem , Muhammad Iqbal and Amer Jamil
  Antimicrobial agents were produced using specific strain of Bacillus subtilis by fermentation of molasses in medium containing MgSO4.7H2O, CuSO4.7H2O and KH2PO4 under semi-shake conditions at 37EC (pH 7.2). Alluding to the individual effect of the salts against E. coil the same conc. of MgSO4.7H2O, (0.05%) had similar impact both after 48 hrs and 72 hrs incubation, while for the other two salts lCuSO4.7H2O and KH2PO4), an increase in conc. concomitantly enhanced the antimicrobial activity only during delayed incubation. Similarly, against Streptococcus pyogenes, all the three salts had better results at delayed span of time (72 hrs). For dual salt combinations MgSO4.7H2O/CuSO4.7H2O and MgSO4.7H2O/KH2PO4 in relative conc. of 0.005/0.1 and 0.005/0.5 against S. pyogenes and MgSO4.7H2O/CuSO4 in conc. of 0.05/0.1 against E. coil yielded better effect after 72 hrs. incubation. In case of triple salt combinations too, 72 hrs incubation proved suitable for better yield of antimicrobial agents. Maximum antimicrobial activity was found at relative salt conc. of 0.005, 0.05 and 0.5% of MgSO4.7H2O, CuSO4.7H2O and KH2PO4 respectively. The extract did not show remarkable antifungal activity against Fusarium oxysporum. No haemolytic activity of the broth extract was found against human erythrocytes.
  Amer Jamil , Muhammad Yaqub , Munir A. Sheikh and Harold G. Martinson
  Cleavage and polyadenylation is an obligatory step in mRNA biogenesis in eukaryotes. Poly(A) tails play an important role in mRNA turn over, transport and translation. Currently available methods for the study of polyadenylation are very cumbersome and involve radioactivity. Dual luciferase assay was found to be an efficient and equally reliable method to CAT assay and RNase protection assay. Comparison of these methods is reported in this paper.
  Tayyba Tabassum Baig , Munir Ahmad Sheikh , Amjad Hameed , Amer Jamil , Faiz Batool , Shazia Muhammad Ali and Shafaqat Ali
  Filter Press Cake (Mud) was enriched with biomass protein using Arachniotus sp. as fermentative organism. The Mud was dewaxed for increased protein production before fermentation. Proximate analysis of native and biomass was done. It was found that after fermentation ash contents decreased from 17.50 to 16.00 % and fiber was decreased from 28 to 2%. Ether extract remained the same. Prior to the production of biomass protein certain conditions like fermentation period (72 h), substrate to water ratio (6%), (NH4)2SO4(0.1%), CaCl2.2H2O (0.025%), MgSO4.7H2O (0.015%), KH2PO4 (0.1%), Cane molasses (1.5%), and Corn Steep Liquor (2.0%) were optimized. These conditions were then applied to produce biomass protein on large scale. The biomass thus produced contained 26.25% crude protein, 13.12% true protein, 6.73% ether extract, 16% ash and 2% crude fiber. Amino acid analysis of biomass revealed the presence of 15 amino acids. The chemical score of protein was `0` and Leucine and Valine were first and second limiting amino acids respectively. The protein quality of biomass was tested in terms of digestibility that showed an average digestibility of 80.917%.
  Semee Mumtaz , Salim - Ur - Rehman , Nuzhat Huma , Amer Jamil and Haq Nawaz
  Enrichment of yoghurt with xylooligosaccharide (XO) at different levels was studied with physicochemical and sensory analysis. Yoghurt prepared by incorporation of XO were compared for these characteristics to the yoghurt containing stabilizer (gelatin, 0.4% w/w) in addition to XO. Moisture contents, pH, acidity and total solids were studied. These attributes were significantly affected by the use of stabilizer and rate of XO incorporation. Use of gelatin produced better results in terms of lowering syneresis and improved appearance, body and texture. Addition of XO upto 3.5% did not influence taste and overall acceptability but higher levels contributed aftertaste.
  Saima Hafeez Khan , Masood Sadiq Butt , Faqir Muhammad Anjum and Amer Jamil
  Rice bran from ‘Basmati Super’ cultivar was stabilized by dry heat, microwave heat and parboiling. All the stabilization techniques were found effective in reduction of antinutrients including trypsin inhibitor, haemagglutinin-lectin and phytates. No adverse effect of stabilization was observed on chemical composition of rice bran. Protein was extracted from differently stabilized rice bran along with unstabilized bran by enzymatic extraction. Protein isolates yield remained highest for the unstabilized bran, followed by Microwave Stabilized Rice Bran Protein Isolates (MWRBPI), Dry Heat Stabilized Rice Bran Protein Isolates (DHRBPI) and Parboiled Rice Bran Protein Isolates (PAR-RBPI).
  Muhammad Tauseef Sultan , Masood Sadiq Butt , Faqir Muhammad Anjum and Amer Jamil
  The cardiovascular disorders are major cause of mortality in diabetic patients and myocardial necrosis, atherosclerosis and high blood pressure are major ailments in this regard. The core objective of the present research study was to explore the role of Black Cumin Seed Fixed Oil (BCFO) and Black Cumin Essential Oil (BCEO) on the markers of myocardial necrosis i.e. cardiac enzymes in normal and diabetic Sprague dawley rats. Diabetes mellitus was induced using streptozotocin @ 60 mg/Kg body weight. The three diets were prepared i.e. D1 (control), D2 (BCFO @ 4.0%) and D3 (BCEO @ 0.30%) and added separately in the diets and fed to the respective groups for a period of 56 days. The results indicated that diets were insignificant and level of cardiac enzymes remained in the safe ranges in normal rats. However, diabetes affected deleteriously and markers of myocardial necrosis increased in control group. In comparison, BCFO and BCEO normalized the levels of markers of myocardial necrosis. However, BCEO was more effective in reduced the elevated levels of these enzymes. Moreover, increased levels of transferases in diabetic rats were also reduced as a function of BCFO and BCEO treatment. In the nutshell, it can be assumed that black cumin essential oil is more effective in reducing the elevated levels of enzymes thus reducing the onset of cardiovascular disorders in diabetes mellitus.
  Semee Mumtaz , Salim-ur- Rehman , Nuzhat Huma and Amer Jamil
  The effect of Xylooligosaccharide (XO) enriched yogurt in addition to basal diet on serum biochemical profile of albino rats assigned to 5 dietary treatments for a period of 21 days were studied. In all groups of rats (n = 5) serum glucose, cholesterol and triglycerides were observed. The weight gain in rats receiving yogurt was higher than those in control group. XO enriched yogurt showed significant improvement in minerals absorption. There was no significant change in serum cholesterol level of rats fed on yogurt with or without XO enrichment but a fair reduction in serum glucose content was noted. XO as a functional ingredient can be incorporated in formulating dairy products with improved health benefits like lowering glucose levels.
  Rabia shabir , Masood Sadiq Butt , Nuzhat Huma and Amer Jamil
  Increasing awareness regarding natural ingredients has led to utilization of functional beverages in diet based therapy. Present project was designed to evaluate safe use of functional drink prepared from green tea active ingredients. Efficacy trial was conducted in male Sprague Dawley rats for period of eight weeks. Functional drinks were prepared by adding catechins and Epigallocatechin Gallate (EGCG) @ 550 mg/500 mL and provided to rats for the period of eight weeks. Four types of studies were conducted consisting of different types of diets i.e. study I (normal diet), study II (high cholesterol diet), study III (high sucrose diet), study IV (high cholesterol+high sucrose diet). The results revealed safety of functional drinks as values for liver and kidney function tests and serum proteins remained in normal range. Organs to body weight ratio were non-significantly effected by functional drinks. Conclusively it can be suggested that functional drinks carrying green tea catechins and EGCG are safe and could be a part of diet therapy for treatments of lifestyle related disorders.
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