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Biotechnology
Year: 2004  |  Volume: 3  |  Issue: 1  |  Page No.: 63 - 66

Molecular Cloning of β-glucosidase Gene from Trichoderma harzianum

Nighat Aslam, Farooq Latif, Sibtain Ahmed and Amer Jamil    

Abstract: Trichoderma harzianum (E-58) was grown on Vogel`s medium containing glucose and xylan as carbon source. Glucose repressed the β-glucosidase production while xylan produced the enzyme in substantial amounts. Total RNA was isolated from mycelia of Trichoderma harzianum grown in medium containing xylan as carbon source. RT-PCR was performed on the isolated RNA. cDNA strand was synthesized by using oligo (dT)18 primer. Specific primers were used to amplify bgl gene from cDNA. The bgl gene was ligated at SmaI site of pUC18 and transformed into E. coli.

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