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Pakistan Journal of Biological Sciences
Year: 2000  |  Volume: 3  |  Issue: 9  |  Page No.: 1475 - 1479

Dual Luciferase Assay: An Efficient Method to Study the Polyadenylation Process

Amer Jamil, Muhammad Yaqub, Munir A. Sheikh and Harold G. Martinson    

Abstract: Cleavage and polyadenylation is an obligatory step in mRNA biogenesis in eukaryotes. Poly(A) tails play an important role in mRNA turn over, transport and translation. Currently available methods for the study of polyadenylation are very cumbersome and involve radioactivity. Dual luciferase assay was found to be an efficient and equally reliable method to CAT assay and RNase protection assay. Comparison of these methods is reported in this paper.

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