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Research Journal of Environmental Sciences
  Year: 2007 | Volume: 1 | Issue: 2 | Page No.: 77-81
DOI: 10.3923/rjes.2007.77.81
Cytosolic Reduction of Toxic Cr (VI) by Indigenous Microorganism
Shaiza Iftikhar, Muhammad Faisal and Shahida Hasnain

Abstract:
In the present study chromium resistant bacterial strain Bacillus cereus S-6 isolated from effluents of tannery was used for the reduction of toxic Cr (VI) into less toxic Cr (III). It could resist very high concentration of K2CrO4 i.e., up to 40 mg mL-1 on nutrient agar, 25 mg mL-1 in nutrient broth and up to 1.5 mg mL-1 of K2CrO4 in acetate-minimal medium. At an initial Cr (VI) concentration of 100 μg mL-1, the cytosol and membrane preparation of the strain were able to reduced almost 67 and 43% of Cr (VI) within 24 h incubation period while the heat killed cytosol and membrane preparation reduced 24 and 18% within the same time period. At high initial K2CrO4 concentration (500 μg mL-1), the reduction percentage decreased and cytosol reduce 36% and membrane preparation 18% of the total chromium supplied after 24 h. After heat shock these reduction values were 13 and 9%, respectively.
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14 January, 2011
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