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Research Journal of Medicinal Plants

Year: 2012 | Volume: 6 | Issue: 7 | Page No.: 489-499
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Authors

Norhazana Norizan

Norizan Ahmat

Sharifah Aminah Syed Mohamad

Nur Ainaa Atikah Mohd Nazri

Siti Saidatul Akmar Ramli

Sariza Natrah Mohd Kasim

Wan Zuraida Wan Mohd Zain

Keywords

Five Shorea species which are endemic plants of Malaysia were used in this study. The methanol extract of Shorea acuminata, Shorea macroptera, Shorea leprosula, Shorea bracteolata and Shorea resinosa were investigated for total phenolic contents (TPC), total flavonoid contents, radical scavenging properties (DPPH), in vitro antioxidant and antimicrobial activities. For the antimicrobial activity, the extracts were tested against four bacteria; Gram negative bacteria Escherichia coli, Gram positive bacteria Staphylococcus pyogenes, Staphylococcus aureus and Bacillus subtilis and two fungi, Candida albicans and Aspergillus niger. The result of TPC analysis showed that S. acuminata displayed highest total phenolic content, 2731±0.09 mg/100 g, while in total flavonoid content, S. resinosa displayed the highest amount of flavonoid, 956.73±0.01 mg/100 g. However, in DPPH analysis, the samples of S. resinosa, S. macroptera and S. acuminate displayed better scavenging activity as compared to butylatedhydroxytoluene (BHT). The in vitro antioxidant activity indicated that S. macroptera showed the highest percent inhibition both in ferric thiocyanate method (FTC) (98.68%) and thiobarbituric acid method (TBA) analysis. In antimicrobial activity, S. resinosa displayed moderate inhibition against Gram negative bacteria Escherichia coli, Gram positive bacteria Streptococcus pyogenes, Staphylococcus aureus and Bacillus subtilis. Other plants were found to display selective inhibition against microbes.
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Norhazana Norizan, Norizan Ahmat, Sharifah Aminah Syed Mohamad, Nur Ainaa Atikah Mohd Nazri, Siti Saidatul Akmar Ramli, Sariza Natrah Mohd Kasim and Wan Zuraida Wan Mohd Zain, 2012. Total Phenolic Content, Total Flavonoid Content, Antioxidant and Antimicrobial Activities of Malaysian Shorea. Research Journal of Medicinal Plants, 6: 489-499.

URL: https://scialert.net/abstract/?doi=rjmp.2012.489.499

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