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Journal of Pharmacology and Toxicology

Year: 2011 | Volume: 6 | Issue: 7 | Page No.: 610-623
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Authors

R. Chandra Besra

L. Majhee

J. Sharma

Keywords

Research Article

Evaluation of Phytochemical, Antioxidant and Hepatoprotective Activity of Tuber of Geodorum laxiflorum Griff

R. Chandra Besra, L. Majhee and J. Sharma
Liver diseases are one of the major causes of mortality and morbidity in humans. Incidence and prevalence of hepatotoxicity due to drugs are increasing and is one of the main contributing factor of death. Herbs of medicinal value are being used since ancient times for the treatment of liver diseases. Orchid plants have ornamental importance but few are known for medicinal use. So, the present study was designed to investigate phytochemical, anti-oxidant and hepatoprotective property of methanolic extract of tuber of Geodorum laxiflorum. Tubers were powdered and extracted with methanol in Soxhlet apparatus to get residue (yield, 25 g, 10%). Phytochemical tests confirmed the presence of flavonoid, polyphenols, sterols and carbohydrates in methanolic extract of the tuber. Chromatographic separation was performed and two major flavonoid were isolated (yield, 25.6% of fraction IV and 31.2% of fraction V). The 50-250 μg mL-1 of extract were evaluated for antioxidant activity in DPPH model. IC50 value of extract was 213 μg mL-1. In acute oral toxicity study, extract was found safe and non-toxic even in higher dose of 2000 mg kg-1 b.wt. Thirty-six healthy adult Wistar albino rats (150-200 g) of either sex were used for the investigation of in vivo hepatoprotective activity of methanolic extract. About 50, 100 and 200 mg kg-1 per oral of extract were used for hepatoprotective activity and compared with 100 mg kg-1 silymarin as standard. Methanolic extract showed prominent reduction in elevated hepatic marker enzymes and significant (p<0.001). In histopathological examination it is observed that extract has significant hepatoprotective activity. In in vitro antioxidant study, extract has shown significant free- radical inhibition activity. Phytochemical analysis of the extract has proved to have flavonoids and polyphenols. In conclusion, the MeOH extract of Geodorum laxiflorum has antioxidant and hepatoprotective activity and contain potent phytochemicals.
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