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Phytochemical, Toxicity, Antimicrobial and antioxidant Screening of Extracts
Obtained from Laportea aestuans (Gaud) |
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Ganiyat K. Oloyede and Olukoyejo E. Ayanbadejo |
Abstract:
Phytochemicals responsible for toxicity, antimicrobial and antioxidant activities
of extracts obtained from Laportea aestuans (Gaud) were investigated.
Secondary metabolites detected were alkaloids, tannins, resins, saponins and
carbohydrate. Flavonoids, sterols, cardiac glycosides, phenols, glycosides were
however beyond detectable limit. Brine shrimp lethality test on the partition
fractions revealed that hexane, ethyl acetate and butanol fractions were toxic
with a lethal dose (LC50) less than 1000 μ mL-1 while
the crude methanol extract with LC50 greater than 1000 μ mL-1
was non-toxic. The antimicrobial assay of the crude extract and their fractions
were carried out by agar well diffusion and pour plate methods against 6 bacteria
(Staphylococcus aureus, Escherichia coli, Bacillus subtilis,
Pseudomonas aeruginosa, Klebsiellae pneumonae, Salmonella typhi)
and 4 fungi (Candida albicans, Aspergillus niger, Rhizopus
stolon, Penicillum notatum). All the extracts had broad spectrum
antimicrobial effect at the various concentrations when compared with gentamicin
and tioconazole (antibacterial and antifungal standards respectively). The antioxidant
activity of L. aestuans was determined by three methods; scavenging effect
on 2,2-diphenyl-1-picryhydrazyl radical (DPPH), hydroxyl radical scavenging
effect and ferric thiocynate methods and it was revealed the fractions possessed
significant antioxidant activity when compared with antioxidant standards butylated
hydroxyl anisole (BHA), ascorbic acid and α-tocopherol used in the assay.
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How to cite this article:
Ganiyat K. Oloyede and Olukoyejo E. Ayanbadejo, 2014. Phytochemical, Toxicity, Antimicrobial and antioxidant Screening of Extracts
Obtained from Laportea aestuans (Gaud). Journal of Medical Sciences, 14: 51-59.
DOI: 10.3923/jms.2014.51.59
URL: https://scialert.net/abstract/?doi=jms.2014.51.59
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