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Journal of Medical Sciences
  Year: 2003 | Volume: 3 | Issue: 2 | Page No.: 163-168
DOI: 10.3923/jms.2003.163.168
 
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A Convenient Colorimetric Assay Method for Determination of Vitamin B12 content in Pharmaceutical Preparations

Firoj Ahmed, Rebecca Banoo , Ghazi Muhammad Sayedur Rahman and Md. Omar Faruk Khan

Abstract:
A convenient colorimetric assay method for the determination of vitamin B12 content in pharmaceutical preparations has been developed. The proposed method is based on the decomposition of vitamin B12 by HNO3 followed by subsequent formation of a stable colored complex (λ max 435 nm) between the liberated cobalt ion and Nitroso-R-salt. Commercial injectable preparations containing vitamin B12 of three different manufacturers (two were single component injection and one was multi-component injection containing vitamin B1, B6 and B12) were analyzed by the proposed method. All of them were found to meet USP limit. A recovery test was also performed which was in close agreement with that of the USP method. Different statistical parameters such as standard deviation, co- efficient of variation and confidence interval were also determined to validate the method.
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How to cite this article:

Firoj Ahmed, Rebecca Banoo , Ghazi Muhammad Sayedur Rahman and Md. Omar Faruk Khan , 2003. A Convenient Colorimetric Assay Method for Determination of Vitamin B12 content in Pharmaceutical Preparations. Journal of Medical Sciences, 3: 163-168.

DOI: 10.3923/jms.2003.163.168

URL: https://scialert.net/abstract/?doi=jms.2003.163.168

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