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International Journal of Plant Pathology

Year: 2011 | Volume: 2 | Issue: 3 | Page No.: 115-125
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Authors

A. Karthikeyan

M. Sudha

M. Pandiyan

N. Senthil

V.G. Shobana

P. Nagarajan

Keywords

Yellow mosaic disease is one of the most important viral diseases in mungbean caused by Mungbean Yellow Mosaic Virus (MYMV) which leads to severe yield reduction and it necessitates developing MYMV resistant lines for improved crop yield. The present investigation was carried out with an objective of identification of MYMV resistant progenies through Agroinoculation. This technique was employed to examine the F2 individuals, which were derived from a cross between Vamban (Gg) 2 (susceptible) x KMG 189 (resistant) to screen for the MYMV resistant progenies. In the field condition, MYMV infection can be evaluated by MYMV disease rating scale (1-9). Out of the 203 F2 individuals, 30 individuals were identified as resistant to MYMV and subjected to agroinoculation. The two tandem viral constructs of MYMV, VA 221 (KA30 DNA A + KA22 DNA B) and VA 239 (KA30 DNA A + KA27 DNA B) mobilized in Agrobacterium tumefaciens strains Ach 5 and C 58 were used for Agroinoculation. The results shows that among the thirty individuals, only five individuals namely MYMVR 10, MYMVR 13, MYMVR 15, MYMVR 17 and MYMVR 37 exhibited resistance to VA 221 strain and found to be susceptible to VA 239 strain. Other twenty five individuals are susceptible to both strains, VA 221 and VA 239. The presence of viral DNA in agroinoculated plants were confirmed by Reverse Transcriptase-Polymerase Chain Reaction (PCR) analysis. For additional corroboration, these five resistant individuals are forwarded to F3 generation and confirmed for resistance in field condition.
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