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International Journal of Botany

Year: 2010 | Volume: 6 | Issue: 4 | Page No.: 394-403
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Authors

G.S. Nagananda

Arijit Das

Sourav Bhattacharya

T. Kalpana

Keywords

Research Article

In vitro Studies on the Effects of Biofertilizers (Azotobacter and Rhizobium) on Seed Germination and Development of Trigonella foenum-graecum L. using a Novel Glass Marble containing Liquid Medium

G.S. Nagananda, Arijit Das, Sourav Bhattacharya and T. Kalpana
Biofertilizers are the formulations of living microorganisms, which are capable of fixing atmospheric nitrogen in the soil and thereby, increasing the crop yield. Trigonella foenum-graecum L. is a medicinally important plant possessing anti-diabetic, anti-cancerous, anti-microbial and hypocholesterolaemic properties. The present study was conducted to develop an in vitro method for studying the effects of biofertilizers (Azotobacter and Rhizobium) on the seed germination and development of Trigonella foenum graecum L. using a simple and cost-effective liquid culture medium containing glass marbles as reusable and biologically inert support matrix. Sucrose optimization studies revealed maximum development for the plantlets grown on 1X Murashige and Skoog liquid medium containing 4% sucrose and glass marbles. Azotobacter and Rhizobium were isolated from rhizosphere soil and root nodules of Trigonella plants, respectively and identified following the standard procedures. Mass cultivation of the bacteria carried out for 5 days reported counts of 2.3x104 cells mL-1. The harvested bacterial cells were used to coat the seeds in the presence and absence of charcoal. After 15 days of growth under in vitro conditions, the root length, shoot length, fresh weight, protein, carbohydrate and chlorophyll contents of the plantlets were determined. Maximum growth was observed for the plantlets grown on 1X MS medium with 4% sucrose and glass marbles, inoculated with 40% concentration of Azotobacter, Rhizobium and their co-inoculum mixed with charcoal. Field trials, conducted under green house conditions, revealed that 10% biofertilizer co-inoculum supported maximum growth of the plants when the seeds were coated with charcoal.
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How to cite this article

G.S. Nagananda, Arijit Das, Sourav Bhattacharya and T. Kalpana, 2010. In vitro Studies on the Effects of Biofertilizers (Azotobacter and Rhizobium) on Seed Germination and Development of Trigonella foenum-graecum L. using a Novel Glass Marble containing Liquid Medium. International Journal of Botany, 6: 394-403.

DOI: 10.3923/ijb.2010.394.403

URL: https://scialert.net/abstract/?doi=ijb.2010.394.403

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