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Biotechnology

Year: 2007 | Volume: 6 | Issue: 2 | Page No.: 268-272
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Authors

A.H. Kabir

M.A. Bari

A.K.M.N. Huda

M.A. Rezvy

I. Mahfuz

Keywords

Research Article

Effect of Growth Regulators and Carbon Sources on Axillary Shoot Proliferation from Shoot-Tip Explant and Successful Transplantation of Papaya (Carica papaya L.)

A.H. Kabir, M.A. Bari, A.K.M.N. Huda, M.A. Rezvy and I. Mahfuz
The investigation was based on direct shoot formation from shoot tip explant, subsequent morphogenesis and rooting of the in vitro proliferated shoots and transplantation of regenerated plants under ex vitro condition. Through proper growth regulators, it was possible to differentiate multiple shoots from shoot tip of papaya (Shahi). In the present investigation, it was observed that shoot proliferation was best in MS medium containing BAP 1.0 mg L-1+KIN 0.5 mg L-1 and BAP 1.0 mg L-1+NAA 0.5 mg L-1. Maximum number of shoot per culture (28.2) and length of the largest shoot (1.7 cm) was found on MS medium supplemented with BAP 1.0 mg L-1+KIN 0.5 mg L-1. Shoot proliferation efficiency was found the best in 11 weeks old explant in MS medium containing BAP 1.0 mg L-1+KIN 0.5 mg L-1. Sucrose in 30 g L-1 concentration as carbon source was proved to be best for shoot proliferation (82%). Maximum number of shoot per culture (41.2) and maximum length of shoot were obtained in the aforesaid concentration of carbon source. For adventitious rooting of regenerated shoot, MS medium containing IBA 1.0 mg L-1+NAA 0.5 mg L-1 was found to be the best. In this growth regulator, treatment 92% of rooting was observed. Longest length of the root (7.9 ± 0.121 cm) was found on MS medium supplemented with IBA 1.0 mg L-1+IAA 0.5 mg L-1. After transplanting the 20 days old rooted shoots into garden soil, compost and sand (2:1:1), 80% of survivability after 5 weeks was achieved.
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