This study was conducted due to the lack of water quality data about Al Qilt drainage basin. Twenty water samples from the five springs in Al Qilt drainage basin were analyzed to determine their quality. Chemical, physical and biological studies were conducted between November (2004) and March (2007) in Wadi Al Qilt drainage basin. Based on physical parameters, the water flowing from the springs is considered as good source of drinking water. According to the results of SAR and TH, the water is classified as suitable source for domestic, industrial and agricultural purposes. Results showed moderate levels of major ion and higher concentrations of lead and cadmium than allowable for drinking water in three spring samples, whereas, 47% of the samples were contaminated with fecal coliforms, which indicate the infiltration of pollutants in the recharge area of the springs.