Research Article

Anti-fungal Potential and Brine Shrimp Lethality Assay of in vitro Raised Clones of Celastrus paniculatus

Background and Objective: Celastrus panicultus Willd (Celastraceae) a climbing shrub is well known for its ability to improve memory. Because of its high therapeutic application, it has been over exploited and is now considered as endangered species. The present investigation was focused the anti-fungal potential and brine shrimp lethality assay of leaf extracts of in vitro raised clones with that of their mother plant. Materials and Methods: The powdered leaves were extracted sequentially using a soxhlet apparatus with solvents such as petroleum ether, chloroform and methanol for 24 h. The extracts of both mother plant and in vitro raised clones were tested for anti-fungal assay and brine shrimp lethality assay. Results: The results showed that the methanolic extract of both mother plant and in vitro raised clones were equally and effectively inhibited Phytophthora capsici and Rhizoctonia solani completely. The chloroform and methanol extract showed maximum mortality against Artemia salina. Conclusion: The study revealed the anti-fungal activity and non-toxic nature of the leaf extracts of both mother plant and in vitro raised clones of C. paniculatus.

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Anusha Tharayil Koonapra Sasidharan and Kothanam Kuziyil Elyas, 2019. Anti-fungal Potential and Brine Shrimp Lethality Assay of in vitro Raised Clones of Celastrus paniculatus. Asian Journal of Biological Sciences, 12: 877-883.

URL: https://scialert.net/abstract/?doi=ajbs.2019.877.883

Asian Journal of Biological Sciences

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Anusha Tharayil Koonapra Sasidharan

Kothanam Kuziyil Elyas

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Research Article

Anti-fungal Potential and Brine Shrimp Lethality Assay of in vitro Raised Clones of Celastrus paniculatus

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