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Asian Journal of Biotechnology
  Year: 2012 | Volume: 4 | Issue: 1 | Page No.: 46-52
DOI: 10.3923/ajbkr.2012.46.52
 
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Effect of Medium Strength and Hormones Concentration on Regeneration of Pogostemon cablin using Nodes Explant

W.A.Wan Nurul Hidayah, J.S. Norrizah, S.M. Sharifah Aminah, S.A. Sharipah Ruzaina and P. Faezah

Abstract:
Pogostemon cablin also known as Patchouli belongs to the family Lamiaceae is an important aromatic plant producing patchouli oil from its leaves extraction. The main objective of this study was to develop an effective method for in vitro propagation of Malaysia cultivated P. cablin. The effect of varying the strength of solid MS medium was investigated using nodes explants. Different concentrations of 6-Benzlaminopurine, BAP (0, 0.25, 0.5, 1.0, 2.0 mg L-1) and alpha naphthalene acetic acid, NAA (0, 0.25, 0.5, 1.0, 2.0 mg L-1) were added to both full and half strength MS (Murashige and Skoog) medium for regeneration of this plant. After 4 weeks, the shoots regeneration from the nodal segments was obtained in all medium. Then, clumps of shoots (5 shoots) were separated and transferred to five MS medium concentrations (0.25 mg L-1 BAP, 0.5 mg L-1 BAP, 0.25 BAP/0.25 NAA and 1.0 BAP/0.25 NAA). MS supplemented with 0.25 mg L-1 BAP alone gave the highest number of shoots (32.93±3.93) and length (3.80±0.27 cm) followed by MS supplemented with combination of 1.0 mg L-1 BAP and 0.25 mg L-1 NAA hormones. There was also a significant effect of hormones and media strength on the number of shoots obtained. The statistical analysis done showed that there was very high interaction between both factors of hormones concentration as well as the medium strength. In the other words, the number of shoots obtained to hormones concentration depends on medium strength and vice-versa. In conclusion, in vitro regeneration of P. cablin from node explants in MS medium supplemented with BAP or NAA was successfully obtained. The healthy propagules that were obtained in this research can be used for further procedure of acclimatization.
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How to cite this article:

W.A.Wan Nurul Hidayah, J.S. Norrizah, S.M. Sharifah Aminah, S.A. Sharipah Ruzaina and P. Faezah, 2012. Effect of Medium Strength and Hormones Concentration on Regeneration of Pogostemon cablin using Nodes Explant. Asian Journal of Biotechnology, 4: 46-52.

DOI: 10.3923/ajbkr.2012.46.52

URL: https://scialert.net/abstract/?doi=ajbkr.2012.46.52

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