The Viability and Infectivity of Toxoplasma gondii Tachyzoites in Dairy Products undergoing Food Processing


	Asian Journal of Animal Sciences
	Year: 2011 \| Volume: 5 \| Issue: 3 \| Page No.: 202-207 DOI: 10.3923/ajas.2011.202.207

The Viability and Infectivity of Toxoplasma gondii Tachyzoites in Dairy Products undergoing Food Processing

Q. Asgari, D. Mehrabani, M.H. Motazedian, M. Kalantari, J. Nouroozi and S.J. Adnani Sadati

Abstract: This study was undertaken to evaluate the viability and infectivity of the tachyzoites in different temperatures and times of milk by bioassay methods. Tachyzoites of RH strain were transferred into sterile milk and kept in different temperatures (4, 20 and 37°C) for different time intervals (10 and 30 min) and their viability was evaluated by vital stain (methylene blue) and the infectivity by intra-peritoneal and oral inoculation to different groups of inbred BALB/C mice (2-4 mice in each group obtained from Pasteur Institute, Tehran, Iran). Tachyzoites in saline were intra-peritoneally inoculated to inbred BALB/C mice as positive controls. All animals were kept in Laboratory Animal Center of Shiraz University of Medical Sciences in Shiraz, southern Iran. The touch smear of spleen and liver of dead mice were stained by Giemsa and were parasitologically evaluated. Eighty percent of tachyzoites were stained by methylene blue indicating identical parasite viability. All mice died after intra-preritoneal inoculation of tachyzoites present in sterile milk. Only one out of eight mice which was orally inoculated remained alive. All mice fed by tachyzoites in saline died. Duration of mice vitality was more in oral route than peritoneal inoculation (n₁ = 11, Mean₁ = 9.55 days and n₂= 17, Mean₂ = 6.65 days, p = 0.001). Tachyzoites in drinks and foods undergoing heating up to 37°C for 30 min such as milk can be a possible route of transmission of toxoplasmosis to human. Therefore strict public health measures should be carried out to prevent transmission of disease in high risk areas.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Seroprevalence of Free-Ranging Chicken Toxoplasmosis in Sub-Urban Regions of Shiraz, Iran

•	The Seroprevalence of Bovine Toxoplasmosis in Fars Province, Southern Iran

•	The Seroprevalence of Ovine Toxoplasmosis in Fars Province, Southern Iran

How to cite this article:

Q. Asgari, D. Mehrabani, M.H. Motazedian, M. Kalantari, J. Nouroozi and S.J. Adnani Sadati, 2011. The Viability and Infectivity of Toxoplasma gondii Tachyzoites in Dairy Products undergoing Food Processing. Asian Journal of Animal Sciences, 5: 202-207.

DOI: 10.3923/ajas.2011.202.207

URL: https://scialert.net/abstract/?doi=ajas.2011.202.207

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		FSTA
		Google Scholar
		ISI Web of Knowledge
		Thomson ISI

Follow @scialert

Science Alert