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Articles by Mohammad Asgharzadeh
Total Records ( 10 ) for Mohammad Asgharzadeh
  Mohammad Asgharzadeh , Saber Yousefee , Hossein Samadi Kafil , Mohammad Reza Nahaei , Khalil Ansarin and Mohammad Taghi Akhi
  To investigate the genetic variation among Mycobacterium tuberculosis isolates from East and West Azarbaijan provinces of Iran and to evaluate the manner of recent transmission of tuberculosis (TB), we performed IS6110-based restriction fragment length polymorphism analysis of isolates. Restriction fragment length polymorphism (RFLP) typing performed on 165 culture-positive specimens from East and West Azarbaijan. Using IS6110 as a probe, Mycobacterium tuberculosis strains assigned to clusters based on identical DNA fingerprints. Rates of patients have clustered were 27.68% in East and 30.19% in West Azarbaijan. There was not statistically significant differences in clustering of patients in two provinces (p = 0.4533) but infection with Mycobacterium tuberculosis in males and females in two provinces were different (p = 0.0048). In East Azarbaijan there was not difference in transmission of tuberculosis between males and females, also in males and females belonged to clusters we couldn’t find statistical difference (p = 0.1833). The rate of active transmission of TB in West Azarbaijan was slightly more than East Azarbaijan. It can be due to different factors such as poor economic and less developed condition in West Azarbaijan.
  Mohammad Asgharzadeh and Hossein Samadi Kafil
  Mannose-Binding Lectin (MBL) is a member of the collectin family. It binds to various oligosaccharides and activates the classical pathway of complement independent from C1q. The aim of present study is to study the distribution of the alleles of MBL gene and promoter variants in intracellular, extracellular pathogens and autoimmune diseases. Our studies showed occurrence of the codon 54 mutation (allele B) of MBL was associated with the occurrence of the acute hepatitis C, this showed the low MBL level can intense progress of this infection, but in other intracellular infections, low expression MBL genotypes associated with protection against these infections and wild type alleles with high MBL production considered as a risk factor for these intracellular pathogens. In extracellular pathogens, there was contrary and wild types of genotype with high production of MBL were associated with protection against these infections and alleles with low MBL production considered as a risk factor for these pathogens. In autoimmune diseases our study and other studies demonstrated that low MBL was a risk factor for these diseases.
  Mohammad Asgharzadeh , Hossein Samadi Kafil , Mohammad Ebrahim Ebrahimzadeh and Aboulfazl Bohlouli
  Systemic Lupus Erythemattosus (SLE) is a prototypical auto immuno disease characterized by the production of the auto antibodies, the aim of present study is to determine the distribution of the alleles of Mannose-binding Lectin (MBL) gene codon 52, 54 and 57 and promoter variants H/L, X/Y, P and Q in SLE patients while compares then with normal control and seek correlation between these variants and disease that cause renal dysfunction. Twelve SLE patients with renal failure samples were compared with thirty normal controls from Azarbaijan population of Iran. MBL genotypes were investigated by polymerase chain reaction and restriction fragment length polymorphism. Allelic and genotypic frequency of the polymorphism at position- 550,+4 and at codon 52, 54 and 57 did not show statistical differences between SLE patients and controls but frequency of Lx haplotype of promoter was observed in patients with SLE and Renal failure (p = 0.0518). Present findings showed that presence of LX haplotype that cause low concentration of MBL in serum can de a risk factor for severity of systemic Lupus Erythematosus and susceptibility to renal dysfunctions.
  Esmaeel Fallah , Kareem Hatam Nahavandi , Rasul Jamali , Behroz Mahdavi Poor and Mohammad Asgharzadeh
  The purpose of this study was to investigate the genotypes of Giardia duodenalis from human and animal feces and their epidemiological in Iran. Molecular characterization of cysts of human and animal origin represents an objective means to approve or reject this hypothesis. In this study, cysts of Giardia duodenalis were collected from feces of naturally infected cats (n = 2), human (n = 34), dog (n = 2) and cattle (n = 3). PCR-RFLP analysis of the 34 specimens recovered from humans revealed 6 G. duodenalis assemblage AII, 8 G. duodenalis assemblage BIII and 4 G. duodenalis assemblage BIV. Among samples from cats, 1 was classified into assemblage AI. Genetic subgenotypes identified from human reveals that genetic diversity of this protozoan in East Azerbaijan, Iran, is similar to that of Giardia from other parts of the world. The present study represents the first contribution to the knowledge of G. duodenalis genotypes in Iran.
  Esmaeel Fallah , Kareem H. Nahavandi , Rasul Jamali , Behroz Mahdavi and Mohammad Asgharzadeh
  In this study, 325 stool samples from sporadic cases giardiasis were examined by conventional techniques for parasite diagnosis. A simple and rapid procedure for the extraction of DNA from fecal samples was developed. Triose phosphate isomerase (tim) based PCR assay was applied for definitive identification and genetic characterization of Giardia intestinalis strains collected from Tabriz Reference Laboratory and pediatric Hospital in Tabriz. Among 34 DNA samples extracted, the tim gene was amplified from 31 (91.1%). Of these, 13 (41.9%) samples contained assemblage B, 17 (54.8%) contained assemblage A and one (3.2%) contained a mixture of assemblage A and assemblage B. Of these, three samples (8.8%) were negative. The results indicated that PCR technique provides an applicable and feasible method for detection and identification of Giardia cysts in stool samples. The results of furthermore, demonstrated that Giardia intestinalis assemblage A and B exist in East Azerbaijan province of Iran.
  Mohammad Asgharzadeh , Abdolsamad Mazloumi , Hossein Samadi Kafil and Ardavan Ghazanchaei
  Visceral leishmaniasis is an infectious disease caused by various species of Leishmania and Leishmania infantum is known to be associated with VL in Iran. Different factors can consider risk factors for VL that some remain unknown. The aim of present study is to determine the distribution of the alleles of mannose-binding lectin gene codon 52, 54, 57 and promoter variants H/L, X/Y, P and Q in confirmed VL patients while compares then with normal controls and seek correlation between these variants and confirmed VL patients. Fifty eight confirmed VL patients blood samples were compared with one hundred and twenty normal controls from Azarbaijan population of Iran. MBL genotypes were investigated by polymerase chain reaction and restriction fragment length polymorphism. Allelic and genotypic frequency of the polymorphism at promoters and genes didn't show statistical differences in patients and normal controls, but frequency of alleles with high MBL concentration in VL patients was higher than controls (p = 0.03). We can conclude that normal alleles with high MBL serum level are risk factor for VL and defective alleles have protective role in VL.
  Mohammad Taghi Akhi , Mohammad Reza Nahaei , Mojtaba Nikbakht and Mohammad Asgharzadeh
  The aims of present investigation were to study the nasal carriage rate of MRSA in hospital staff and in-patients, determination of antibiotic resistant patterns of nasal and clinical MRSA isolates and typing of MRSA isolates by RAPD- PCR. Two hundred and six S. aureus isolates were recovered from clinical specimens and noses of 460 staff and in-patients admitted in Imam Khomeini and Pediatrics hospitals by standard methods during 6 months (2004-2005). Disk agar diffusion (using 13 antibiotics disks) and oxacillin agar screening methods for detection of MRSA isolates were performed according to CLSI. PCR was also used to amplify a 310 bp sequence from S. aureus genome (mecA gene) for detection of MRSA isolates. RAPD-PCR was carried out for fingerprinting of MRSA isolates genome. MRSA isolates were resistant up to 11 antibiotics. All of the MRSA isolates were resistant to penicillin, but sensitive to vancomycin. Of 206 S. aureus isolates, 77 MRSA isolates were detected using disk agar diffusion and oxacillin agar screening methods. In contrast, 80 isolates were detected as MRSA by amplification of mecA sequence. In RAPD-PCR experiments, 43 different RAPD patterns were obtained from our MRSA isolates. Nasal carrier rate of S. aureus was 34.7% and MRSA isolates were high (38.8%) in our hospitals. This study revealed high rate of MRSA, that infected patients and MRSA nasal carriers (staff and in-patients) were the main source of transmission and infection, therefore effective control measures are necessary to avoid nosocomial infection outbreaks.
  Mohammad Asgharzadeh , Hossein Samadi Kafil , Mohammad Ebrahim Ebrahimzadeh and Aboulfazl Bohlouli
  The aim of present study is to determine the distribution of the alleles of mannose-binding lectin gene and promoter variants in infections that cause renal dysfunctions. Fifty eight renal recipients’ samples which lost their kidneys in result of infection have compared with 120 normal controls from Azarbaijan population of Iran. Blood samples were obtained from renal transplant recipients who received renal from March 2004 to July 2005. Mannose-binding lectin genotypes have investigated by polymerase chain reaction and restriction fragment length polymorphism. Allelic and genotypic frequency of the polymorphism at position-550, +4 and at codon 52 and 57 did not show statistical differences between infected patients and controls (p>0.05) but significant frequency of allele B (codon 54) (p = 0.0011) and Ly (p = 0.007), Lx haplotype (p = 0.0002) of promoter was observed in this patients and allele A was more frequent in healthy patients. Present findings provide evidence that presence of different alleles and haplotypes that cause low concentration of mannose-binding lectin in serum is a risk factor for susceptibility to renal infections that cause renal dysfunction.
  Mohammad Asgharzadeh , Saber Yousefee , Mohammad Reza Nahaei , Mohammad Taghi Akhi , Khalil Ansarian and Hossein Samadi Kafil
  IS6110-based DNA fingerprinting is currently the most widely used genetic marker for differentiating among Mycobacterium tuberculosis strains. To evaluate the DNA polymorphism among Mycobacterium tuberculosis strains and to determine if there is matching of IS6110 fingerprints representing recent transmission of tuberculosis. Totally one hundred and sixty five isolates of M. tuberculosis (53 from West Azarbaijan and 112 from East Azarbaijan) were analyzed by IS6110 restriction fragment length polymorphism fingerprinting. Isolates having identical RFLP patterns were considered a cluster. The average number of IS6110 copies per strain was 7.3 and ranged from 0 to 17 among the M. tuberculosis isolates. The IS6110-DNA patterns from these isolates were highly polymorphic. In conclusion 123 patterns were observed which 16 patterns were shared by 47 isolates (30.52%). Most strains (93.62%) had multicopy patterns and only 3 of clustered isolates had less than six IS6110 copies. In our study increased clustering was observed with isolates from male patients. RFLP analysis of 154 isolates of M. tuberculosis showed a considerable diversity, suggesting that most patients were infected with unique strains, probably resulted from reactivation of the latent infection.
  Mohammad Reza Nahaei , Yaeghob Sharifi , Mohammad Taghi Akhi , Mohammad Asgharzadeh , Mehrnaz Nahaei and Ebrahim Fatahi
  The aim of this study was to detect cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene A (vacA) genotypes of Helicobacter pylori and to study their relationships to the associated diseases. In the present analytical descriptive study H. pylori isolates were collected from 150 patients who underwent gastro duodenoscopy in Imam Khomeini Hospital of Tabriz, Iran. Of the patients 76 (50.7%) were males and 74 (49.3%) were females. The patients were divided into two groups. Group I consisted of 117 (78%) Non-Ulcerative Dyspepsia (NUD) patients and group II consisted of 33 (22%) Peptic Ulcer Disease (PUD) patients. Extracted DNA of H. pylori isolates were subjected to PCR tests to detect cagA, signal (s) and middle (m) regions of vacA genotypes. The designed primers revealed the presence of cagA gene in 125 (83.3%) of the isolates. Regarding vacA signal sequences 99 (66%) of our isolates revealed s1 type. The proportion of s1a, s1b and s1c subtypes were 76/150 (50.7%), 7/150 (4.7%) and 16/150 (10.6%), respectively while 40/150 (26.7%) presented as s2 type. In further analysis of the m region of vacA, m1 and m2 subtypes were detected in 49/150 (32.7%) and 81/150 (54%) of the isolates, respectively. The m1 subtype were further divided into m1a [41/49 (83.7%)] and m1b [(8/49 (16.3%)]. Thirty one isolates (20.7%) showed more than one vacA alleles in a single patient. Our results showed that isolates carrying the cagA gene were higher in PUD group than in NUD group, but did not substantiate statistically the role of cagA as a marker influencing increased virulence (p>0.05). Present findings also showed that s1 and s2 subtypes of vacA gene are markers which differentiate between PUD and NUD groups.
 
 
 
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