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Articles by Kiran Shafiq
Total Records ( 4 ) for Kiran Shafiq
  Umar Farooq Awan , Kiran Shafiq , Shazia Mirza , Sikander Ali , Asad-ur- Rehman and Ikram-ul- Haq
  Solid-state fermentation for lipase production was carried by Rhizopus oligosporous ISUUV-16. Almond meal was used as basal substrate with distilled water as moistening agent at substrate to diluent ratio of 1:0.7. Fermentation was carried out at 30oC and culture was incubated for 48 h. Maximum lipase activity under solid-state condition was obtained when Tween 80 at the level of 0.5 % was used as additional carbon source. Best organic nitrogen sources for optimal lipolytic activity was ammonium sulfate (4 %). This study would improve the further optimization steps on the bioprocess development track.
  Kiran Shafiq , Sikander Ali and Ikram-ul-Haq
  The effect of different mineral nutrients was studied on the production of extracellular invertases by Saccharomyces cerevisiae GCB-K5. Nutritional studies were carried out in 250ml Erlenmeyer flasks by submerged fermentation. The culture medium (pH 6.0) was incubated for 48 hours at 30oC. The optimum levels of peptone and dipotassium hydrogen phosphate were found to be 4.0 and 0.2 g/l, respectively. Maximum invertase activity was found to be 12.68 U/ml. The amount of sugar consumed and dry cell mass were 21.08 and 5.88g/l, respectively.
  Kiran Shafiq , Sikander Ali , Amna Ehsan and Ikram-ul-Haq
  The present study concerns with the optimization of inoculum level for invertase production by Saccharomyces cerevisiae GCB-K5. The thermal inactivation of yeast invertase and effect of pH, utilizing the measurements of enzyme activity, was also estimated. Nutritional studies were carried out in 250 ml Erlenmeyer flasks by submerged fermentation. The fermentation medium (pH 6.0) was kept at 30°C for 48 h after inoculation and agitation rate was 200 rpm. Enzyme production was reached maximum (7.1 U ml-1) at 4% inoculum (v v-1). Sugar consumption and dry cell mass were 21.13 and 5.93 mg ml-1, respectively. Comparison of product and growth yield coefficients was also significant for invertase biosynthesis.
  Kiran Shafiq , Ikram-ul-Haq and Sikander Ali
  The influence of the age and amount of the inoculum on invertase synthesis by Saccharomyces cerevisiae KR18 was studied during submerged cultivation. Fermentation was carried out at 30oC in fermentation medium containing sucrose, yeast extract and peptone at initial pH 6.0 at 200 rpm. The maximum production of invertase (6.9 U ml-1) was obtained when 1.0 ml vegetative inoculum (4 %), developed for 12 h, was used per 25 ml of fermentation medium. The dry cell mass and sugar consumption were 3.0 and 16.18 mg ml-1, respectively. The specific growth and product rates (h-1) are more encouraging to use 4 % vegetative inoculum under optimized conditions.
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