Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Pakistan Journal of Biological Sciences
Year: 2003  |  Volume: 6  |  Issue: 11  |  Page No.: 952 - 954

Optimization of Cultural Conditions for the Biosynthesis of β-fructofuranosidase by Saccharomyces cerevisiae

Mirza Ahsen Baig, Kiran Shafiq, Sikander Ali, Shazia Mirza, Zafar Siddiq and Ikram-ul-Haq    

Abstract: The enzyme βfructofuranosidase attacks beta-D-fructofuranoside (raffinose, stachyose and sucrose) from the fructose end. Saccharomyces GCA-II was used for the production of βfructofuranosidase by submerged fermentation. The effect of sucrose concentration and incubation time was worked out for maximal enzyme production. Sucrose at the level of 30.0 g l-1 in fermentation medium was found to exert an inducible effect on enzyme synthesis by Saccharomyces strain after 48 h of inoculation. Enzyme production increased from 107.42 U ml-1 to 118.58 U ml-1 after optimization of the cultural conditions. Specific product rate and specific growth rate were also determined for the production of βfructofuranosidase by Saccharomyces in shake flask.

Cited References   |    Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility