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Biotechnology
Year: 2002  |  Volume: 1  |  Issue: 1  |  Page No.: 40 - 44

Effect of Different Mineral Nutrients on Invertase Production by Saccharomyces cerevisiae GCB-K5

Kiran Shafiq, Sikander Ali and Ikram-ul-Haq    

Abstract: The effect of different mineral nutrients was studied on the production of extracellular invertases by Saccharomyces cerevisiae GCB-K5. Nutritional studies were carried out in 250ml Erlenmeyer flasks by submerged fermentation. The culture medium (pH 6.0) was incubated for 48 hours at 30oC. The optimum levels of peptone and dipotassium hydrogen phosphate were found to be 4.0 and 0.2 g/l, respectively. Maximum invertase activity was found to be 12.68 U/ml. The amount of sugar consumed and dry cell mass were 21.08 and 5.88g/l, respectively.

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