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Journal of Biological Sciences
Year: 2003  |  Volume: 3  |  Issue: 2  |  Page No.: 191 - 196

Optimization of Assay Conditions and Inoculum Size with Kinetic Analysis for Biosynthesis of Invertase by Saccharomyces cerevisiae GCB-K5

Kiran Shafiq, Sikander Ali, Amna Ehsan and Ikram-ul-Haq    

Abstract: The present study concerns with the optimization of inoculum level for invertase production by Saccharomyces cerevisiae GCB-K5. The thermal inactivation of yeast invertase and effect of pH, utilizing the measurements of enzyme activity, was also estimated. Nutritional studies were carried out in 250 ml Erlenmeyer flasks by submerged fermentation. The fermentation medium (pH 6.0) was kept at 30°C for 48 h after inoculation and agitation rate was 200 rpm. Enzyme production was reached maximum (7.1 U ml-1) at 4% inoculum (v v-1). Sugar consumption and dry cell mass were 21.13 and 5.93 mg ml-1, respectively. Comparison of product and growth yield coefficients was also significant for invertase biosynthesis.

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