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Articles by Yang Yang
Total Records ( 14 ) for Yang Yang
  Qiuhong Li , Fengzhi Wu , Yang Yang and Xuezheng Wang
  We studied the effects of rotation and interplanting on soil bacterial communities and crop yields using cucumber as the main vegetable. Onion and garlic were used as interplanting species, and wheat, soybean, villose (more commonly, villous) vetch, clover, and alfalfa were used as rotation plants. T-RFLP techniques were used to show the effects of rotation and interplanting systems on diversity index and richness index of cucumber rhizosphere soil bacterial communities. The results showed that both rotation and interplanting systems increased the richness index and diversity index of soil bacterial community structures, except where alfalfa was used as the rotation plant. The diversity index of the cucumber rhizosphere bacterial community structure was highest in the wheat rotation cropping system, and the richness index of soil bacteria was highest in wheat and clover rotations and in the onion interplanting system. Our results show that rotation and interplanting systems beneficially altered community structures of dominant soil bacteria, and increased cucumber yield and soil bacterial diversity. The best cultivation system to increase cucumber yield utilized onion as an interplanting species and wheat as a rotation plant.
  Jin -Hong Qin , Qing Zhang , Zhi- Ming Zhang , Yi Zhong , Yang Yang , Bao- Yu Hu , Guo -Ping Zhao and Xiao- Kui Guo
  DNA microarray analysis was used to compare the differential gene expression profiles between Leptospira interrogans serovar Lai type strain 56601 and its corresponding attenuated strain IPAV. A 22-kb genomic island covering a cluster of 34 genes (i.e., genes LA0186 to LA0219) was actively expressed in both strains but concomitantly upregulated in strain 56601 in contrast to that of IPAV. Reverse transcription-PCR assays proved that the gene cluster comprised five transcripts. Gene annotation of this cluster revealed characteristics of a putative prophage-like remnant with at least 8 of 34 sequences encoding prophage-like proteins, of which the LA0195 protein is probably a putative prophage CI-like regulator. The transcription initiation activities of putative promoter-regulatory sequences of transcripts I, II, and III, all proximal to the LA0195 gene, were further analyzed in the Escherichia coli promoter probe vector pKK232-8 by assaying the reporter chloramphenicol acetyltransferase (CAT) activities. The strong promoter activities of both transcripts I and II indicated by the E. coli CAT assay were well correlated with the in vitro sequence-specific binding of the recombinant LA0195 protein to the corresponding promoter probes detected by the electrophoresis mobility shift assay. On the other hand, the promoter activity of transcript III was very low in E. coli and failed to show active binding to the LA0195 protein in vitro. These results suggested that the LA0195 protein is likely involved in the transcription of transcripts I and II. However, the identical complete DNA sequences of this prophage remnant from these two strains strongly suggests that possible regulatory factors or signal transduction systems residing outside of this region within the genome may be responsible for the differential expression profiling in these two strains.
  Xiaoying Yang , Song, ming , Yang Yang and Huayue Sun
  In order to overcome the problems in current literatures, that is neglect of collaborative effects and lean operation and the simple weighted method for dealing with the relations of quality, duration and cost, the collaborative optimization of planning and control for Large Equipment Service-oriented Manufacturing (LESM) is studied systematically based on lean logistics. Firstly, the necessity of collaborative optimization of planning and control were analyzed, as well as the lean logistics objective and the relationship among three objectives of quality, duration and cost. Secondly, introducing the Taguchi quality loss function and punishment factor, the coordination model of quality-duration-cost was established based on the cost. Thirdly, considering coordination among levels of project and quality-duration-cost, a multi-level and multi-objective coordination optimization model of planning and control was built. Fourthly, integration algorithm of simulation modeling and genetic algorithm (SM and GA) was designed and effectiveness was verified by case study. This provides a theoretical method for LESM planning and control.
  Naijun Han , Yewei Li , Chenglong Sun , Qingguo Meng , Feifei Song , Yang Yang , Shoufeng Zhang and Rongliang Hu
  The rabies virus glycoprotein is an efficient vaccine antigen particularly for the oral vaccine of wildlife. Pseudorabies virus contains many non-essential genes and was usually used to construct recombinant vaccines as a viral vector to deliver foreign genes into the target animals. In this study, a recombinant pseudorabies virus rPRV/SRV9G/EGFP, expressing EGFP and rabies virus (SRV9 strain) glycoprotein was constructed by homologous recombination in the UL21 gene. The immunogenicity was evaluated as a vaccine in mice. In this trial, thirty mice were administered with the recombinant vaccine orally. This recombinant virus was demonstrated to be safe for mice by oral administration and could induce neutralizing antibody level above 0.5 IU mL-1 at 3 weeks after the vaccination. The results suggest that rPRV/SRV9G/EGFP was an effective oral vaccine candidate against rabies and UL21 gene could be a target insertion area for foreign genes.
  Zhijun Zhong , Yongjiu Luo , Yang Yang , Chengdong Wang , Xuehan Liu , Desheng Li , Tingmei He , Wuyang Gu , Fangyuan Wang , Zhiyao Zhou , Tianliang Tang , Shengquan Ai , Gangshi Li , Xiaoxiao Zhou , Xiaoyang Xu , Bingbing Xie , Zhihua Ren , Hemin Zhang , Suizhong Cao , Liuhong Shen , Hualin Fu and Guangneng Peng
  The diet conversion period (1-1.5 years) is a special time for the giant panda. During this period, giant pandas need to adapt from a high-protein diet to highly fibrous bamboo as their main food and form a special digestive system that will digest cellulose and hemicellulose. Previous studies have shown that diet alterations affect intestinal microbiota composition and host resistance. Intestinal microbiotas play a key role in the giant panda’s ability to digest highly fibrous bamboo. In this study, researchers constructed a 16S rRNA gene library from three giant pandas’ feces to investigate the diversity and structure of its bacterial population during the diet conversion period. Results showed that the diversity of intestinal bacteria during the earlier and later diet conversion periods is higher than at the middle diet conversion period. Intestinal floras within the giant panda gut were affiliated with the phyla Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria and uncultured bacterium. The phyla Firmicutes and Proteobacteria were the predominant bacteria throughout diet conversion although their proportions fluctuated. Within the phylum Firmicutes, the majority of bacteria were Clostridium, Streptococcus and Lactobacillus but while in the phylum Proteobacteria, the predominant bacteria were Escherichia and Acetobacter. This is the first study to monitor bacterial diversity in feces from captive giant pandas during the diet conversion period.
  Xingfang Qiao , Li Wei , Yang Yang , Jiankang Chen , Denghu Wu , Benzhong Mi , Wenhua Luo , Suhui Yin and Jinlong Yang
  Asian elephants in Chongqing zoo were repeatedly affected by fungal skin disease recent years. This study sought to establish New Zealand rabbit models infected with Microsporum canis which was pathogens of fungal skin disease developed in Asian elephants in Chongqing zoo. Bacterial colonies were incubated in Sabouraud dextrose agar at 28°C for 14 days and then prepared into bacterial suspension and inoculated on the dorsal skin of the rabbits. After 10 days inoculation, the affected skin of rabbits developed advent clinical symptoms such as scab and desquamation that were extremely similar to those of fungal skin disease in Asian elephants. The skin tissue at a size of 1×1 cm was collected. The paraffin section was prepared and stained for argentaffin. The pathological section showed that brown orange Microsporum canis was visible in skin tissue after argentaffin staining. The results showed that pathological New Zealand rabbit model for infection with pathogens of fungal skin disease developed in Asian elephants in Chongqing zoo was successfully established.
  Shuai Li , Yang Yang , Lai Hui , Xu Chao and Zhou Zonfang
  Nowadays, the assessment of individual credit risk has drawn great attention of the financial institutions and many assessment models have been developed. However, although the traditional assessment models can assess the credit risk from different aspects, they require that the credit information of the credit subjects is complete. Therefore, those traditional models cannot be well suitable for the individual credit assessment. In order to solve this problem, a more suitable model should be established. Compared with the previous work, this study presents an integrated model. The new model integrates the existing assessment models by using SP theory. The integrated model based on SP theory will greatly improve the suitability while with less credit information of the credit subjects. Through a specific example, the validity of the model has been verified. From the findings of this study, it shows that the new assessment model has some theoretical value and practical significance for the assessment of individual credit risk.
  Zhuling Zhong , Yang Yang and Mu Zhang
  Social media are playing an increasingly important role as information sources for travelers and online reviews reconstruct the decision-making of purchase. The goal of this study is to investigate the influence extent of online reviews on consumer’s hotel reservation intention. Based on the data analysis from the online comments, the measurable conceptual model of online comment which affecting the consumer’s hotel reservation intention is built up, the five observed variables and the relevant suppose have been put forward. The model is evaluated according to a national survey of potential consumers. All data have been analyzed with the Structural Equation Model (SEM). The results show that all variables except comment source significantly affected tourists’ hotel reservation intention. It also puts forward a reference case for the tourism company and its websites on improving online reservation service quality to meet customer’s satisfaction.
  Yang Yang , Chuntao Yin , Weizhi Li and Xudong Xu
  Unlike Escherichia coli, the cyanobacterium Synechocystis sp. strain PCC 6803 is insensitive to chill (5°C) in the dark but rapidly losses viability when exposed to chill in the light (100 µmol photons m–2 s–1). Preconditioning at a low temperature (15°C) greatly enhances the chill-light tolerance of Synechocystis sp. strain PCC 6803. This phenomenon is called acquired chill-light tolerance (ACLT). Preconditioned wild-type cells maintained a substantially higher level of α-tocopherol after exposure to chill-light stress. Mutants unable to synthesize α-tocopherol, such as slr1736, slr1737, slr0089, and slr0090 mutants, almost completely lost ACLT. When exposed to chill without light, these mutants showed no or a slight difference from the wild type. When complemented, the slr0089 mutant regained its ACLT. Copper-regulated expression of slr0090 from PpetE controlled the level of α-tocopherol and ACLT. We conclude that α-tocopherol is essential for ACLT of Synechocystis sp. strain PCC 6803. The role of α-tocopherol in ACLT may be based largely on a nonantioxidant activity that is not possessed by other tocopherols or pathway intermediates.
  Yang Yang , Shabbir M. Walijee , Jing Jin , Shui-ping Zhao and Dao-Quan Peng


Apolipoprotein A-V (ApoA-V) has been shown to play an important role in the metabolism of plasma triglyceride (TG) levels. However, data for the relationship between serum ApoA-V and TG level and the association between ApoA-V levels and the risk of coronary artery disease (CAD) are inconsistent.


To investigate plasma ApoA-V level and its association with TG in patients with CAD determined by angiography.


Three hundred forty subjects who underwent coronary angiography (CAG) were classified into a CAD group (n = 211) and a non-CAD group (n = 129) according to the results of their CAG. Serum ApoA-V levels were determined by enzyme-linked immunosorbent assays.


Patients in the CAD group had greater ApoA-V levels than controls (192.0 114.6 ng/mL vs 164.4 107.5 ng/mL, P < .05). The concentration of ApoA-V was positively correlated to TG level (r = 0.229, P < .01). This correlation was more intense in non-CAD group (r = 0.306, P < .01) than in CAD group (r = 0.172, P < .05). ApoA-V was positively associated with Gensini score. However, the contribution of ApoA-V to Gensini score and CAD risk disappeared after adjustment for the TG level.


The positive correlation between serum ApoA-V and TG differs in strength between control and CAD patients. The correlation between ApoA-V and CAD is confounded by the effect of TG. We speculate that the increase of ApoA-V may be a consequence of compensation for the increased demand for plasma TG hydrolysis.

  Li-Peng Wu , Xi Wang , Lian Li , Ying Zhao , Shaoli Lu , Yu Yu , Wen Zhou , Xiangyu Liu , Jing Yang , Zhixin Zheng , Hui Zhang , Jingnan Feng , Yang Yang , Haiying Wang and Wei-Guo Zhu
  Histone deacetylase inhibitor (HDACi) has been shown to demethylate the mammalian genome, which further strengthens the concept that DNA methylation and histone modifications interact in regulation of gene expression. Here, we report that an HDAC inhibitor, depsipeptide, exhibited significant demethylating activity on the promoters of several genes, including p16, SALL3, and GATA4 in human lung cancer cell lines H719 and H23, colon cancer cell line HT-29, and pancreatic cancer cell line PANC1. Although expression of DNA methyltransferase 1 (DNMT1) was not affected by depsipeptide, a decrease in binding of DNMT1 to the promoter of these genes played a dominant role in depsipeptide-induced demethylation and reactivation. Depsipeptide also suppressed expression of histone methyltransferases G9A and SUV39H1, which in turn resulted in a decrease of di- and trimethylated H3K9 around these genes` promoter. Furthermore, both loading of heterochromatin-associated protein 1 (HP1α and HP1β) to methylated H3K9 and binding of DNMT1 to these genes` promoter were significantly reduced in depsipeptide-treated cells. Similar DNA demethylation was induced by another HDAC inhibitor, apicidin, but not by trichostatin A. Our data describe a novel mechanism of HDACi-mediated DNA demethylation via suppression of histone methyltransferases and reduced recruitment of HP1 and DNMT1 to the genes` promoter.
  Haiying Wang , Ying Zhao , Lian Li , Michael A. McNutt , Lipeng Wu , Shaoli Lu , Yu Yu , Wen Zhou , Jingnan Feng , Guolin Chai , Yang Yang and Wei-Guo Zhu
  Most agents that damage DNA act through posttranslational modifications of p53 and activate its downstream targets. However, whether cellular responses to nucleoside analogue-induced DNA damage also operate through p53 posttranslational modification has not been reported. In this study, the relationship between p53 activation and its posttranslational modifications was investigated in the human cancer cell lines A549 and HCT116 in response to 5-aza-2`-deoxycytidine (5-aza-CdR) or cytarabine treatment. 5-Aza-CdR induces p53 posttranslational modifications through activation of an ATM- and Rad3-related (ATR) signaling pathway, and 5-aza-CdR-induced association of replication protein A with chromatin is required for the binding of ATR to chromatin. Upon treatment with 5-aza-CdR, ATR activation is clearly associated with p53 phosphorylation at Ser15, but not at Thr18, Ser20, or Ser37. This specific p53 phosphorylation at Ser15 in turn results in acetylation of p53 at Lys320 and Lys373/Lys382 through transcriptional cofactors p300/CBP-associated factor and p300, respectively. These p53 posttranslational modifications are directly responsible for 5-aza-CdR induced p21Waf1/Cip1 expression because the binding activity of acetylated p53 at Lys320/Lys373/Lys382 to the p21Waf1/Cip1 promoter, as well as p21Waf1/Cip1 expression itself are significantly increased after 5-aza-CdR treatment. It is of interest that p53 phosphorylation at Ser15 and acetylations at Lys320/Lys373/Lys382 mutually interact in the 5-aza-CdR induced p21Waf1/Cip1 expression shown by transfection of artificially mutated p53 expression vectors including S15A, K320R, and K373R/K382R into p53-null H1299 cells. These data taken together show for the first time that 5-aza-CdR activates the ATR signaling pathway, which elicits a specific p53 phosphorylation-acetylation cascade to induce p21Waf1/Cip1 expression.

  Yun Shi , Xianfeng Chen , Zhongying Wu , Weiwei Shi , Yang Yang , Ningren Cui , Chun Jiang and Robert W. Harrison
  Vascular ATP-sensitive K+ channels are activated by multiple vasodilating hormones and neurotransmitters via PKA. A critical PKA phosphorylation site (Ser-1387) is found in the second nucleotide-binding domain (NBD2) of the SUR2B subunit. To understand how phosphorylation at Ser-1387 leads to changes in channel activity, we modeled the SUR2B using a newly crystallized ABC protein SAV1866. The model showed that Ser-1387 was located on the interface of NBD2 with TMD1 and physically interacted with Tyr-506 in TMD1. A positively charged residue (Arg-1462) in NBD2 was revealed in the close vicinity of Ser-1387. Mutation of either of these three residues abolished PKA-dependent channel activation. Molecular dynamics simulations suggested that Ser-1387, Tyr-506, and Arg-1462 formed a compact triad upon Ser-1387 phosphorylation, leading to reshaping of the NBD2 interface and movements of NBD2 and TMD1. Restriction of the interdomain movements by engineering a disulfide bond between TMD1 and NBD2 prevented the channel activation in a redox-dependent manner. Thus, a channel-gating mechanism is suggested through enhancing the NBD-TMD coupling efficiency following Ser-1387 phosphorylation, which is shared by multiple vasodilators.
  Anurag Purushothaman , Ligong Chen , Yang Yang and Ralph D. Sanderson
  High levels of heparanase are an indicator of poor prognosis in myeloma patients, and up-regulation of the enzyme enhances tumor growth, angiogenesis, and metastasis in animal models. At least part of the impact of heparanase in driving the aggressive tumor phenotype is due to its effect on increasing the expression and shedding of the heparan sulfate proteoglycan syndecan-1, a molecule known to promote myeloma progression. The present work demonstrated that elevation in heparanase expression in myeloma cells stimulates sustained ERK phosphorylation that in turn drives MMP-9 expression. In addition, urokinase-type plasminogen activator (uPA) and uPA receptor expression levels increased, and blocking the proteolytic activation of either MMP-9 or uPA inhibited the heparanase-induced increase in syndecan-1 shedding. Together these data provide a mechanism for heparanase-induced syndecan-1 shedding and, more importantly, demonstrate that heparanase activity in myeloma cells can lead to increased levels of proteases that are known to play important roles in the aggressive behavior of myeloma tumors. This in addition to its other known biological roles, indicates that heparanase acts as a master regulator of the aggressive tumor phenotype by up-regulating protease expression and activity within the tumor microenvironment.
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