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Articles by Xiuli Peng
Total Records ( 7 ) for Xiuli Peng
  Shijun Li , Zhenhua Liang , Cui Wang , Yanping Feng , Xiuli Peng and Yanzhang Gong
  In the present study, we investigated the effect of squalene on the reproductive performance in meat-type male chicken. A total of 480 meat-type males, which were 72 weeks old with body weight of 6.0±0.5 kg were assigned randomly and equally to 4 groups in two independental experiments which represent two classic mating types AI (Artificial Insemination) and NM (Natural Mating), respectively. Each experiment contains two groups, control group and squalene-treated group in which meat-type males were fed with the supplemental squalene at a daily dose 10 mg/kg/day for 45 days. Semen data such as average collection semen volume, sperm count and egg fertile rate were collected from experiment I that was designed keeping males and females seperated and using artificial insemination as mating type. In the experiment I that designed keeping birds together in floor and using natural mating as mating type, only fertility data were collected. Also, serum testosterone levels were measured in squalene-treated, control and males no ejaculation groups. In the experiment I, the average collection semen volume is 393 μL, which is significantly higher (p<0.001) than the control group, which is 287 μL. The egg fertile rate has no significant difference between these two groups. In experiment II, the egg fertile rate of squalene treated group was significantly higher than control group (p<0.001).The serum testosterone levels of squalene treated group was significantly higher (p<0.05) than control group. The results showed that meat-type males treated with squalene increased the serum testosterone level and semen collection volume and did not decrease egg fertile rate in AI model and also increased the egg fertile rate in natural mating model. These findings have implications that squalene has a prompt effect on reproductive performance on meat-type male chicken.
  Zhenhua Liang , Cui Wang , Huawen Yu , Xiuli Peng , Yanping Feng , Yanzhang Gong and Shijun Li
  The tyrosinase family is known to be crucial in the melanin biosynthetic pathway and is responsible for the rate limiting step. In the present study, the complementary DNA (cDNA) of TYP1 was cloned from the eye of duck by homology cloning and rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of TYP1 consisted of 2123 nucleotides, containing an Open Reading Frame (ORF) of 1608 bp that encoding a 536 amino-acid peptide, a 5'-terminal Untranslated Region (UTR) of 255 bp and a 3'-terminal UTR of 260 bp with two canonical polyadenylation signal sequence (AATAAA) and a poly(A) tail. The phylogenetic tree display that TYP1 protein is highly conserved and the deduced peptide shares 70.9-93.7% similarity with quail, chicken and mammalian TYP1 proteins. The Semiquantitative RT-PCR analysis indicated that the transcripts of TYP1 mRNA had the highest expression in eyes and black hair follicle, intermediate in white hair follicle and negligible or absent in skin, muscle, heart, liver, kidneys, spleen, lungs, brain and intestine. The study may useful for the further study on polymorphism and correlation on duck feather color and the function of TYP1 of birds.
  Shijun Li , Wenhua Yu , Cui Wang , Zhenhua Liang , Xiuli Peng , Yanping Feng and Yanzhang Gong
  Stem Cell Factor (SCF) also called Steel Factor (SF), Mast cell Growth Factor (MGF) and KIT tyrosine Kinase receptor Ligand (KL) is a hematopoietic growth factor and ligand for the KIT tyrosine kinase receptor. In this study, the partial complementary DNA (cDNA) of SCF was cloned from the eye of duck by homology cloning and Rapid Amplification of Cdna Ends (RACE) approaches. Comparing with zebra finch, quail, chicken and mammalian SCF cDNA sequence, the phylogenetic tree displayed that duck SCF is highly conserved, it shares 63.8-94.3% similarity with the above species cDNA sequence. The rooted phylogenetic neighbor-Joining tree with bootstrap was done using SCF CDS sequences from duck and other species by DNAMAN software. The results showed that duck has close relationship with chicken, quail and zebro finch. The semi-quantitative RT-PCR analysis indicated that SCF was a universally expressed gene, it was detected in heart, liver, spleen, kidney, brain, back skin, muscle, eye, glandular stomach, belly skin. Q-PCR was employed to analyze the SCF gene expression in six type duck hair bulbs. The results showed that its expression had significant difference between W-W which is white hair bulb taken from white plumage with yellow beak and other types (BL-Bl which is black feather hair bulb taken from black plumage with black beak, W-L which is white feather hair bulb taken from white plumage with black beak ducks, W-WB which is white feather taken from white-black plumage with black beak, B-WB which is black feather taken from white-black plumage with black beak), p<0.001). The expression of SCF in BR (brown feather hair bulb taken from brown plumages with black beak ducks) has significant difference comparing with the above 5 hair bulb types (p<0.05). There is no significant difference between any of the following types which including BL-BL, W-L, W-WB and B-WB. The results indicated that SCF may be a critical gene on regulation gene expression for duck plumage diversity.
  Xian Gao , Lixia Zhou , Ganzhen Deng , Chang Liu , Chengye Li , Changwei Qiu and Xiuli Peng
  To determine which aerobic bacteria exert a marked effect on Cystic Endometrial Hyperplasia-Pyometra complex (CEH-P) and to determine from where these bacteria originate. About 48 privately owned bitches were diagnosed with CEH-P. Healthy vagina and uterine samples were obtained with cotton pledgets and cumulative uterine fluids from CEH-P cysts were collected with a syringe in utero to isolate and identify aerobic bacterial. A total of 99 isolates were identified and the primary aerobe in the CEH-P bitches was found to be Escherichia coli (33/99). The levels of estrode in bitches with endometritis and in bitches with pyometra and estrus were higher than the levels in diestrus bitches (p<0.01). When ranked according to the levels of progesterone in a descending order (i.e., from high to low levels), the ranking was bitches with pyometra, bitches with endometritis, bitches in estrus and bitches in diestrus. These results suggest that in addition to common pathogenic bacteria differential in every area, casual bacterial contamination invariably occurred in bitches with CEH-P and the primary infection ascended from the vagina when the cervix was open. The researchers also inferred that both estrode and progesterone levels in CEH-P bitches were increased relative to the levels in healthy bitches and were even higher than that of the levels observed in estrus bitches. Estrode and progesterone pretreatment and uterine mucosa damage were interpreted as the preconditions for bacterial infection. The hormone levels in CEH-P bitches were similar to the endocrinal characteristics of peripartum bitches.
  Xiuli Peng , B. Cao , G.Z. Deng , C.Y. Li , L.L. Ye and H. Yu
  The object of this study was to describe the echography characteristics of different abnormal ovaries and estimate their effect on dairy cow sterility. The ovaries of 74 infertile dairy cows were checked with transrectal real-time ultrasound (7.0 MHz) and echography characters of abnormal ovaries were described. Thereinto, ovarian abnormality occurred about 60% and the other 40% case hadn’t obvious echogenic change. Hypoplasia showed a little area within parenchyma anecho or poor echo which could discriminate it with any other ovarian abnormality. Even though appeared a little echo area, atrophic ovary had much little high echo dot. Although, quiescent ovary was also very little, it had some small follicles which couldn’t develop. Ultrasound image showed large liquid anecho area of large ovaries in cystic ovarian follicle and corpora lutea cases. Anecho area of cystic ovarian follicle had echo of cumulus oophorous and its wall smooth. Corpus luteum appeared much little poor echo dot in crude fluid-filled anecho area and some of them had anecho cavity. Ovaritis showed an enlarged echo area much hyper or strong echo spot distributed inside. The edge of ovary connected tightly with circumjacent tissues reflecting hyper-echo. Few little follicles appeared but no dominant follicle and luteum were scanned.
  Xiuli Peng , Shen Zhang , Ganzhen Deng , Beibei Li , Yue Wu , Mingyue He , Xiao Wang , Chengye Li , Changwei Qiu and Kechun Zhang
  Major histocompatibility complex takes the capital contribution to maternal immune responses to the semiallogenic fetus. As the member of BoLA-Ib, the machenism of class I chain-related genes 2 (MIC2) on pregnant setup and delivery, till remains to be elucidated. To evaluate the possible effect of MIC2 on pregnant establishment and delivery, neonatal ear tissues, fetal placenta, peripheral blood Polymorphonuclear Neutrophils (PMN) of dairy cow in different pregnant stages were collected to analyze MIC2 expression by quantitative RT-PCR. MIC2 on PMN in dairy cows was down regulated expressed in 1st and 2nd trimesters and recoveried back to normal level in peripartum. Moreover, MIC2 was novel upregulated expressed in neonatal ear tissues and downregulated expressed in fetal placenta. These results suggested that MIC2 lowly expressed in the 1st and 2nd trimester of pregnant cows could suppress NKG2D binding MIC2 on PMN and therefore, let embryo escape from maternal immune response. Anabiotic MIC2 expression in peripartum suggested that maternal immune to full grown foetus recover back to normal level which be in favour of delivery. In foetus, novel upregulated expression of MIC2 in tissues was favourible for foetus to escape its autoimmunity. Whereas, downregulated expression in fetal placenta let placenta decoherenced from maternal placentome which was beneficial to delivery.
  Yongping Yang , Ping Gong , Shijun Li , Xiuli Peng , Yanping Feng and Yanzhang Gong
  Lipoprotein Lipase (LPL) is a multifunctional protein that plays a major role in the hydrolysis of triglycerides present in chylomicrons and very low-density lipoproteins. This study was designed to investigate the effects of Single Nucleotide Polymorphisms (SNPs) of the duck LPL gene on fatness and carcass and growth traits. A White Kaiya x White Liancheng F2 population with a total of 1069 individuals was used in the present study, 440 healthy ducks randomly selected from this population were slaughtered at 80 days of age. PCR-Single Strand Conformation Polymorphism (PCR-SSCP) and sequencing methods were used to detect SNPs in the duck LPL gene. Two new SNPs (C645T and G726A) were discovered in exons 5 and genotyped using the PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Method. Genotyping results showed that the genotype distribution differed between males and females, thus the association analyses were performed separately for males and females. Association analyses indicated that the SNPs were significantly associated with body weight, fatness and carcass traits (p<0.05 or p<0.01). We concluded that LPL is a major gene or is linked with a major gene that influences body weight, fatness and carcass traits and that C645T and G726A could be used as candidate molecular genetic markers for breeding selection.
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