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Journal of Animal and Veterinary Advances

Year: 2010  |  Volume: 9  |  Issue: 16  |  Page No.: 2102 - 2108

Molecular Cloning, Characterization and Expression Analysis of Duck Tyrosinase-Related Protein-1

Zhenhua Liang, Cui Wang, Huawen Yu, Xiuli Peng, Yanping Feng, Yanzhang Gong and Shijun Li

Abstract

The tyrosinase family is known to be crucial in the melanin biosynthetic pathway and is responsible for the rate limiting step. In the present study, the complementary DNA (cDNA) of TYP1 was cloned from the eye of duck by homology cloning and rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of TYP1 consisted of 2123 nucleotides, containing an Open Reading Frame (ORF) of 1608 bp that encoding a 536 amino-acid peptide, a 5'-terminal Untranslated Region (UTR) of 255 bp and a 3'-terminal UTR of 260 bp with two canonical polyadenylation signal sequence (AATAAA) and a poly(A) tail. The phylogenetic tree display that TYP1 protein is highly conserved and the deduced peptide shares 70.9-93.7% similarity with quail, chicken and mammalian TYP1 proteins. The Semiquantitative RT-PCR analysis indicated that the transcripts of TYP1 mRNA had the highest expression in eyes and black hair follicle, intermediate in white hair follicle and negligible or absent in skin, muscle, heart, liver, kidneys, spleen, lungs, brain and intestine. The study may useful for the further study on polymorphism and correlation on duck feather color and the function of TYP1 of birds.

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