Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the
coverage of the most important and influential journals
to meet the needs of the global scientific community.
The shoots of Rauvolfia serpentina from the field growing plants were used as explants and cultured on MS medium supplemented with 2.5 mg/L BA + 0.1 mg/L NAA to afford multiple shoots. It was evident that MS + 0.5 mg/L BA + 0.2 mg/L NAA was the most suitable combination for induction of callus and development of shoot buds formation but it failed to support elongation of shoot. So, the cultures with tiny shoots was transferred to 2.0 mg/L BA with 0.05mg/L NAA, where they showed excellent elongation. Regenerated shoots rooted well 0.2 mg/L IBA+ 0.2mg/L NAA combination. After having acclimatization the regenerated plantlets were transferred to the open field where 95% of the plant-lets survived and showed excellent growth.
Phytochemical screening and alkaloid estimation of in vitro and field grown plants of R. serpentina has been investigated. Under the phytochemical screening moisture content (at 45 and 105oC), biomass yield and alkaloid content in vitro and field grown plant materials were determined. At 45oC in vitro plant materials (leaf, root and callus) have higher moisture content compared with field grown (3 years mature plants) plant materials (leaf and root). But opposite result was observed at 105oC. Percent yield of crude plant extract (Biomass yield) of in vitro plant materials was higher than that of field grown plant materials. The percentage of alkaloid for in vitro plant materials was 1.17% in callus, 0.98% in root and 0.49% in leaf, respectively. On the other hand it was found that the field grown plant materials were 0.71% in leaf and 1.43% in root, respectively.
