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Pakistan Journal of Biological Sciences

Year: 2002  |  Volume: 5  |  Issue: 1  |  Page No.: 75 - 79

Micropropagation and Plant Regeneration of Rauvolfia serpentina by Tissue Culture Technique

Shahrear Ahmad, M. N. Amin, M.A.K. Azad and M. Ashik Mosaddik

Abstract

The shoots of Rauvolfia serpentina from the field growing plants were used as explants and cultured on MS medium supplemented with 2.5 mg/L BA + 0.1 mg/L NAA to afford multiple shoots. It was evident that MS + 0.5 mg/L BA + 0.2 mg/L NAA was the most suitable combination for induction of callus and development of shoot buds formation but it failed to support elongation of shoot. So, the cultures with tiny shoots was transferred to 2.0 mg/L BA with 0.05mg/L NAA, where they showed excellent elongation. Regenerated shoots rooted well 0.2 mg/L IBA+ 0.2mg/L NAA combination. After having acclimatization the regenerated plantlets were transferred to the open field where 95% of the plant-lets survived and showed excellent growth.

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