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Pakistan Journal of Biological Sciences
Year: 2002  |  Volume: 5  |  Issue: 4  |  Page No.: 416 - 418

Studies on Moisture Content, Biomass Yield (Crude Plant Extract) and Alkaloid Estimation of In vitro and Field Grown Plants of Rauvolfia serpentina

Shahrear Ahmad, M.N. Amin and M. Ashik Mosaddik    

Abstract: Phytochemical screening and alkaloid estimation of in vitro and field grown plants of R. serpentina has been investigated. Under the phytochemical screening moisture content (at 45 and 105oC), biomass yield and alkaloid content in vitro and field grown plant materials were determined. At 45oC in vitro plant materials (leaf, root and callus) have higher moisture content compared with field grown (3 years mature plants) plant materials (leaf and root). But opposite result was observed at 105oC. Percent yield of crude plant extract (Biomass yield) of in vitro plant materials was higher than that of field grown plant materials. The percentage of alkaloid for in vitro plant materials was 1.17% in callus, 0.98% in root and 0.49% in leaf, respectively. On the other hand it was found that the field grown plant materials were 0.71% in leaf and 1.43% in root, respectively.

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