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Articles by S. Bunnag
Total Records ( 4 ) for S. Bunnag
  P. Maneerattanarungroj , S. Bunnag and M. Monthatong
  Protocorm cryopreservation has been performed for ex situ preservation of Cleisostoma areitinum (Rchb.f.) Garay, rare Thai orchid species. Cryopreservation of protocorms was investigated by an encapsulation-dehydration method. The highest regrowth percentage of cryopreserved explants was observed when they were precultured on semi-solid ND medium with 0.25 M sucrose in the dark for 1 week then transferred to liquid ND medium containing 0.75 M sucrose for 2 days. The treated explants were dehydrated with silica gel in laminar air-flow for 5 h prior to immersion in liquid nitrogen for 1 h. Protocorm viability was tested by the TTC (2, 3, 5-triphenyltetrazoliumchloride) assay and regrowth ability was assessed by determining the survival percentage after recovery for 2 weeks. The survival rate of cryopreserved protocorms was 49% while percentage of TTC assay was 77%. Consequently, the simple and reliable protocol for cryopreservation of C. areitinum was clearly revealed in this study.
  K. Rattana , P. Theerakulpisut and S. Bunnag
  Abiotic and biotic stresses are the limiting factors of growth and productivity of rice in many agriculture areas of Thailand. Plant Tissue cultures have been used for breeding purpose, especially in selection for stress tolerance. The aim of this study was to investigate and find out enhancement on callus induction and regeneration in rice cultivars; Khao Dawk Mali 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1. The suitable media for callus induction of KDML 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1 were NN medium supplemented with 2, 1.5, 2.5 and 1 mg L-1 2,4-D, respectively. It was found that addition of 300 mg L-1 casein hydrolysate enhanced callus formation in KDML 105. Combination with 300 mg L-1 casein hydrolysate and 1,000 mg L-1 L-proline can influence for callus induction in Supanburi 1. Addition of 500 mg L-1 L-proline can enhance callus induction of Chai Nat 1 and Pathum Thani 1. The MS medium supplemented with 3 mg L-1 BA and 300 mg L-1 casein hydrolysate was suitable for regeneration of KDML 105. The most suitable medium for regeneration in Supanburi 1 was MS medium combination with 3 mg L-1 BA, 0.5 mg L-1 NAA and 500 mg L-1 L-proline. Addition of 5 mg L-1 BA, 0.5 mg L-1 NAA and combination with 300 mg L-1 casein hydrolysate in MS medium were the optimal for regeneration in Chai Nat 1. Combination with 5 mg L-1 BA and 300 mg L-1 casein hydrolysate can influence for regeneration in Pathum Thani 1.
  J. Hongthongkham and S. Bunnag
  An efficient method for in vitro propagation and cryopreservation of Aerides odorata was established. Leaf segments were cultured on New Dogashima (ND) mediums supplemented with various concentrations of Benzyladenine (BA) (0-5 mg L-1) combined with Naphthaleneacetic Acid (NAA) (0-2 mg L-1). The optimal treatment for inducing Protocorm-like Bodies (PLBs) from leaf segments was obtained from the combination of 1 or 3 mg L-1 BA and 0.5 or 1 mg L-1 NAA; whereas, the addition of BA or NAA alone induced shoot and/or root initiation rather than PLB or callus formation. Shoots rapidly developed on ND mediums containing 5 mg L-1 BA. Cryopreservation of leaf segment-derived PLBs was successful using the encapsulation-dehydration method. The maximum survival percentage of Cryopreserved (Cryp) PLBs was achieved by encapsulating PLBs with 2% Na-alginate combined with 2 M glycerol and 0.4 M sucrose. The encapsulated PLBs were then precultured in 0.75 M sucrose for 24 h and dehydrated for 6 h before plunging into liquid nitrogen. Genetic stability of Cryp PLBs after regrowth was assessed by flow cytometry. The findings showed no different patterns of ploidy levels and morphology between Cryp and non-cryopreserved (Ncryp) control plantlets.
  K. Duangsee and S. Bunnag
  The potential for callus induction and regeneration depends on nutrient composition and plant growth regulators. The aim of the present study was to investigate the effect of nutrient composition and plant growth regulators on callus induction and plant regeneration in the glutinous rice cultivar Khunvang. The effect of 2,4-D concentrations (1, 2, 3, 4 and 5 mg L-1) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (97%) was observed in MS medium supplemented with 5 mg L-1 2,4-D under 16 h Photoperiod. The effects of casein hydrolysate concentrations of casein hydrolysate (0, 300, 500, 700 and 900 mg L-1) and proline (0, 300, 500, 700 and 900 mg L-1) on callus induction and growth of Khunvang were also observed. The results indicated that the increasing casein hydrolysate and proline concentrations did not show a significant effect on callus growth. However, proline concentration of 900 mg L-1 yielded 85.67% of callus growth.
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