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Asian Journal of Plant Sciences
Year: 2012  |  Volume: 11  |  Issue: 4  |  Page No.: 182 - 189

The Effect of Plant Growth Regulators and Organic Supplements on Callus Induction and Plant Regeneration in Rice (Oryza sativa L.)

K. Rattana, P. Theerakulpisut and S. Bunnag    

Abstract: Abiotic and biotic stresses are the limiting factors of growth and productivity of rice in many agriculture areas of Thailand. Plant Tissue cultures have been used for breeding purpose, especially in selection for stress tolerance. The aim of this study was to investigate and find out enhancement on callus induction and regeneration in rice cultivars; Khao Dawk Mali 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1. The suitable media for callus induction of KDML 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1 were NN medium supplemented with 2, 1.5, 2.5 and 1 mg L-1 2,4-D, respectively. It was found that addition of 300 mg L-1 casein hydrolysate enhanced callus formation in KDML 105. Combination with 300 mg L-1 casein hydrolysate and 1,000 mg L-1 L-proline can influence for callus induction in Supanburi 1. Addition of 500 mg L-1 L-proline can enhance callus induction of Chai Nat 1 and Pathum Thani 1. The MS medium supplemented with 3 mg L-1 BA and 300 mg L-1 casein hydrolysate was suitable for regeneration of KDML 105. The most suitable medium for regeneration in Supanburi 1 was MS medium combination with 3 mg L-1 BA, 0.5 mg L-1 NAA and 500 mg L-1 L-proline. Addition of 5 mg L-1 BA, 0.5 mg L-1 NAA and combination with 300 mg L-1 casein hydrolysate in MS medium were the optimal for regeneration in Chai Nat 1. Combination with 5 mg L-1 BA and 300 mg L-1 casein hydrolysate can influence for regeneration in Pathum Thani 1.

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