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Pakistan Journal of Biological Sciences
Year: 2014  |  Volume: 17  |  Issue: 1  |  Page No.: 98 - 103

Influence of Nutrient Composition and Plant Growth Regulators on Callus Induction and Plant Regeneration in Glutinous Rice (Oryza sativa L.)

K. Duangsee and S. Bunnag    

Abstract: The potential for callus induction and regeneration depends on nutrient composition and plant growth regulators. The aim of the present study was to investigate the effect of nutrient composition and plant growth regulators on callus induction and plant regeneration in the glutinous rice cultivar Khunvang. The effect of 2,4-D concentrations (1, 2, 3, 4 and 5 mg L-1) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (97%) was observed in MS medium supplemented with 5 mg L-1 2,4-D under 16 h Photoperiod. The effects of casein hydrolysate concentrations of casein hydrolysate (0, 300, 500, 700 and 900 mg L-1) and proline (0, 300, 500, 700 and 900 mg L-1) on callus induction and growth of Khunvang were also observed. The results indicated that the increasing casein hydrolysate and proline concentrations did not show a significant effect on callus growth. However, proline concentration of 900 mg L-1 yielded 85.67% of callus growth.

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