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Articles by B. Ramanujam
Total Records ( 2 ) for B. Ramanujam
  Ali Derakhshan , R.J. Rabindra and B. Ramanujam
  Studies were conducted on the effect of culture media, storage temperature and moisture content on viability and virulence of Verticillium lecanii to Brevicoryne brassicae. Among nine media evaluated, Molasses Yeast Broth (MYB) plus 2% Polyethylene Glycol (PEG) maintained the fungus viability higher than other media followed by MYB plus 1% PEG and rice powder. Viability in refrigerator temperature was significantly higher than in room temperature. Among three moisture levels tested, viability at 5 and 10% were on par and were significantly higher than at 15%. Viability over time decreased and the differences in viability among the three storage times were significant. Storage time and media had significant effect on aphid mortality. Mortality decreased over storage time but the rate of decrease in aphid mortality was less than the rate of decrease in the fungal viability.
  Ali Derakhshan , R.J. Rabindra , B. Ramanujam and Mehdi Rahimi
  For mass production of V. lecanii, three types of cultivation methods including liquid, solid and diphasic production systems were investigated. In the liquid state of production, six media were tested in stationary culture conditions. Among the six media tested, Molasses Yeast Broth (MYB) supported maximum sporulation (8.33x108 spores mL-1) and biomass production (746 mg/100 mL). In the MYB, 4% molasses concentration was found to produce highest spore count (8.56x108 spores mL-1) and biomass (776 mg/100 mL) followed by 5 and 6% molasses. Among the six solid substrates tested, rice grains supported highest spore production (1.14 g/100 g). In diphasic state of production, combination of MYB and rice grains produced the greatest amount of spores, (1.70 g/100 g). Results of this study indicated that diphasic method using MYB as liquid medium and rice as solid substrate are the best method and media for mass production of Vl-7 isolate of V. lecanii.
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