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Journal of Biological Sciences

Year: 2008  |  Volume: 8  |  Issue: 2  |  Page No.: 498 - 501

Effect of Storage Conditions of Formulations on Viability of Verticillium lecanii (Zimmerman) Viegas and its Virulence to Brevicoryne brassicae (L.)

Ali Derakhshan, R.J. Rabindra and B. Ramanujam


Studies were conducted on the effect of culture media, storage temperature and moisture content on viability and virulence of Verticillium lecanii to Brevicoryne brassicae. Among nine media evaluated, Molasses Yeast Broth (MYB) plus 2% Polyethylene Glycol (PEG) maintained the fungus viability higher than other media followed by MYB plus 1% PEG and rice powder. Viability in refrigerator temperature was significantly higher than in room temperature. Among three moisture levels tested, viability at 5 and 10% were on par and were significantly higher than at 15%. Viability over time decreased and the differences in viability among the three storage times were significant. Storage time and media had significant effect on aphid mortality. Mortality decreased over storage time but the rate of decrease in aphid mortality was less than the rate of decrease in the fungal viability.

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