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Pakistan Journal of Biological Sciences
Year: 2008  |  Volume: 11  |  Issue: 11  |  Page No.: 1506 - 1509

Evaluation of Different Media and Methods of Cultivation on the Production and Viability of Entomopathogenic Fungi, Verticillium lecanii (Zimm.) Viegas

Ali Derakhshan, R.J. Rabindra, B. Ramanujam and Mehdi Rahimi    

Abstract: For mass production of V. lecanii, three types of cultivation methods including liquid, solid and diphasic production systems were investigated. In the liquid state of production, six media were tested in stationary culture conditions. Among the six media tested, Molasses Yeast Broth (MYB) supported maximum sporulation (8.33x108 spores mL-1) and biomass production (746 mg/100 mL). In the MYB, 4% molasses concentration was found to produce highest spore count (8.56x108 spores mL-1) and biomass (776 mg/100 mL) followed by 5 and 6% molasses. Among the six solid substrates tested, rice grains supported highest spore production (1.14 g/100 g). In diphasic state of production, combination of MYB and rice grains produced the greatest amount of spores, (1.70 g/100 g). Results of this study indicated that diphasic method using MYB as liquid medium and rice as solid substrate are the best method and media for mass production of Vl-7 isolate of V. lecanii.

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