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Articles by A.M. Kholif
Total Records ( 12 ) for A.M. Kholif
  A.S. Gad , A.M. Kholif and A.F. Sayed
  The objective of this study was to use date palm syrup as a part of water (v/v) used in reconstituting skim milk powder in processing yogurt with 14% total solids. Physical properties such as sensory characteristics and apparent viscosity were evaluated. To evaluate the nutritional value of yogurt, antioxidant values were monitored during storage and the sample which recorded the highest values would determine its chemical composition. In addition, some micronutrients (HCl-soluble minerals) and (folate and C vitamins) compared to plain yogurt. Results showed that yogurt enriched with 10% date syrup had a significant sweetness, recorded the highest antioxidant values, higher in HCl-soluble minerals and folate concentration compared to plain yogurt. It could be concluded that numerous health benefits beyond its nutritional value have been associated with consuming yogurt enriched with 10% date palm syrup.
  H.H. Azzaz , A.M. Kholif , H.A. Murad , M.A. Hanfy and M.H. Abdel Gawad
  An in vitro study was conducted to evaluate the effect of cellulases addition to banana wastes on dry matter (IVDMD) and organic matter (IVOMD) disappearances. Laboratory produced cellulase (Asperozym) and a commercial cellulolytic enzyme source (Bacillozym®) were added separately to banana wastes at 4 levels (0, 0.77, 1.54, 2.31 and 3.08 Unit/kg DM). Increasing the Asperozym levels up to 3.08 U kg-1 DM exhibited the highest (p<0.05) IVDMD and IVOMD, while Bacillozym® recorded the highest (p<0.05) IVDMD and IVOMD values at 1.54 U kg-1 DM compared with the untreated banana wastes (Control). Nine lactating Zaraibi goats (about 3 years old and weight on average 31±0.2 kg) after parturition were divided into three groups of three animals each, using 3x3 Latin square designs to evaluate the effect of Asperozym and Bacillozym® addition to diets on the productivity of lactating goats. Animals were fed on 50% Concentrate Feed Mixture (CFM), 25% banana wastes and 25% berseem (clover) straw (control diet). Control diet+ Asperozym at level of 3.08 U kg-1 DM (T1); control diet+Bacillozym® at level of 1.54 U kg-1 DM. (T2). Apparent digestibility for all nutrients were improved (p<0.05) by cellulases treatments. Milk and 4% Fat Corrected Milk (FCM) yields were higher (p<0.05) for T1 group followed by T2 group than control group while milk composition was not affected (p<0.05). Blood plasma Aspartate aminotransferase (AST) and glucose concentration were not affected by treatments. The addition of Asperozym and Bacillozym® to diets improved the performances of lactating Zaraibi goats with no deleterious effects on general health.
  M.A. Abdel-Wahhab and A.M. Kholif
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  M.A. Abdel-Wahhab and A.M. Kholif
  Mycotoxins are secondary metabolites produced by moulds, mostly belonging to the three genera Aspergillus, Penicillium and Fusarium. They are produced in cereal grains as well as forages before, during and after harvest, in various environmental conditions. Mycotoxins generally display great chemical heterogeneity and approximately 400 of these fungal metabolites are considered to be toxic. Mycotoxin metabolism is complex and involves pathways of bioactivation and detoxification in both humans and animals. Detoxification occurs via biotransformation mediated by enzymes in the host cells and in the digestive microbial flora. Some of the toxins or their metabolites may become fixed in animal or human tissues. However, most are eliminated in the urine, faeces and milk. In animals, toxicity is generally revealed as chronic minor troubles and only rarely causes death. The presence of mycotoxins in feeds may decrease feed intake and affect animal performance. In addition, the possible presence of toxic residues in edible animal products (milk, meat, offal), may have some detrimental effects on human health. Maximum acceptable doses in feeds and milk have been set for certain mycotoxins by international authorities. The potential risks of mycotoxins may be controlled by checking plant material for fungal contamination, by improving methods of cultivation, harvest and storage, by eliminating or diluting toxins from the contaminated food or feeds and by using adsorbents to reduce the bioavailability of toxins in the digestive tracts of animals.
  A.M. Kholif , Eman S.A. Farahat , M.A. Hanafy , S.M. Kholif and R.R. EL-Sayed
  Two experiments were carried out to evaluate the effects of cellulases supplementation on in vitro degradation of date kernels (the first trial) and in vivo (rumen fermentation and nutrients digestibility) by lactating Zaraibi goats (the second trial). In the in vitro experiment, dry matter and organic matter disappearance (IVDMD and IVOMD) were determined for date kernels supplemented separately with (Asperozym) and commercial cellulolytic enzyme source (Veta-Zyme Plus®) at 3 levels (15, 30 and 45 U kg–1 DM) compared with the control. The highest values (p<0.05) of IVDMD and IVOMD were observed with Asperozym supplementation level at 45 U kg–1 DM compared to control. While, Veta - Zyme Plus® gave the highest (p<0.05) IVDMD and IVOMD values at 15 U kg–1 DM compared to control. In the in vivo experiment, nine lactating Zaraibi goats after 7 days of parturition were divided into three groups, three animals each, using 3×3 Latin square designs. The first group was fed 37.5% Concentrate Feed Mixture (CFM), 12.5% date kernel and 50% berseem hay (control diet). The second group was fed control diet supplemented with Veta-Zyme Plus® at level 15 U kg–1 DM (T1). The third group was fed control diet supplemented with Asperozyme at level 45 U kg–1 DM (T2). The results indicated that Asperozym and Veta-Zyme Plus® supplementation significantly (p<0.05) increased nutrients digestibility, nutritive values, ruminal Total Volatile Fatty Acids (TVFA’s) and ruminal ammonia nitrogen (NH3-N) for treated groups compared with the control group.
  H.A. Murad , A.M. Abd El Tawab , A.M. Kholif , S.A. Abo El-Nor , O.H. Matloup , M.M. Khorshed and H.M. El-Sayed
  Tannase production by Aspergillus niger was evaluated under solid-state fermentation and submerged fermentation cultures. The optimum conditions for maximum enzyme production including deferent inoculum ratios, incubation periods, initial pH values, nitrogen and carbon sources were investigated. A. niger was grown as stand cultures in 250 mL conical flasks containing tannic acid powder medium. The maximum production of tannase by A. niger was achieved at inoculum ratio of 2% (v/v), 96 h of incubation period, initial pH 5.0, yeast extract as a nitrogen sources at a concentration of 0.33 g N L-1 and palm kernel powder (PKP) as a carbon source at a concentration of 25% (w/v). PKP was found to be the best carbon source supporting production of 931.27 U L-1 min-1 compared with 6.25 U L-1 min-1 for wheat straw.
  Mervat I. Foda , S.M. Kholif and A.M. Kholif
  The aim of this study was to evaluate the effect of supplementing goat’s ration with different forms of lysine and methionine on milk production, its chemical composition and consumer’s acceptability. To maximize goat milk benefits, different concentration of β-D-galactosidase were used to form galacto-oligosaccharides after supplementing the ration with lysine and methionine in protected and unprotected forms. Milk acceptability and its chemical composition were evaluated compared to control samples. The results showed that milk fat, lactose, total protein and milk yield were increased by adding the amino acids in both different forms. Organoleptic evaluation indicated significant increase in taste and overall acceptability of goat milk containing higher concentration of galactooligosaccharides. Also, supplementing goat ration with these amino acids should be recommended to increase milk production and its contents. Formation of galactooligosaccharides in goat milk could be recommended to decrease most of infant’s allergy and diseases.
  H.M. Khattab , H.M. Gado , A.E. Kholif , A.M. Mansour and A.M. Kholif
  Upgrading the nutritive value of sun dried Rumen Contents (RC) by enzymatic treatments and additives can make it a valuable feed resource for ruminants. The objective of this study was to determine the feeding value of partial replacement of Berseem Clover (BC) by treated RC with a mixture of exogenous enzymes (ZADO® or ZAD®) from anaerobic bacteria in the ration of early lactating Baladi goats. Twelve lactating Baladi goats weighed 26±0.5 kg in the first week of lactation were randomly assigned among four experimental treatments using 4x4 Latin square design to be fed four rations. 60% Concentrate Feed Mixture (CFM)+40% BC (Control); 60% CFM+20% BC+20% DRC (T1); 60% CFM+20% BC+20% DRC treated with compound ZAD (T2); 60% CFM+20% BC+20% DRC treated with ZAD compound+20 g compound ZADO /head/d fed directly before feeding (T3). The period of this trial divided into four experimental periods each of 30 days. Results showed that T3 and T2 groups recorded higher values of digestibility coefficients compared with control and T1 group. Groups contained DRC recorded higher values (p>0.05) of ruminal pH and non Protein Nitrogen (NPN) than the control. The treated groups (T2, T3) showed higher (p<0.05) values for rumen liquor ammonia, NPN and total volatile fatty acids (TVFA's) (p>0.05) compared with untreated group (T1). Results showed insignificant differences for blood serum total proteins, globulins, urea, Creatinine, Serum aspartate aminotransferase (AST), Alanin aminotransferase (ALT) and glucose. Biological treated groups (T3 and T2) increased (p>0.05) daily milk yield, 4%fat corrected milk (4% FCM), fat, Total Solids (TS), Solids Not Fat (SNF) and lactose yields compared with T1 group. It could be concluded that feeding goats on rations containing DRC treated with ZADO and/or ZAD compounds as a partial substitute of berseem improved the performance of lactating goats without any adverse effect on animals’ health.
  A.M. Abd El Tawab , O.H. Matloup , A.M. Kholif , S.A.H. Abo El-Nor , H.A. Murad , H.M. El-Sayed and M.M. Khorshed
  Two experiments were carried out to evaluate the effects of laboratory produced tannase enzyme (Tanozym) to diet including Palm Kernel Powder (PKP) on in vitro dry matter and organic matter disappearance (IVDMD and IVOMD) and in vivo nutrients digestibility, nutritive values, milk production and composition by lactating Baladi goats. In vitro experiment, IVDMD and IVOMD, were determined for control diets (60% CFM and 40% Berseem hay); (T1) control diet plus different levels of polyethylene glycol (PEG), being 10, 15 and 20 g kg-1 DM and (T2) control diet plus different levels of Tanozym (3.9, 5.85 and 7.8 U kg-1 DM). The maximum IVDMD and IVOMD values were observed with 5.85 U kg-1 DM for Tanozym and 20 g kg-1 DM for PEG compared to control, however there was no significant (p<0.05) difference between 15 and 20 g kg-1 DM. The in vivo experiment was carried out on nine lactating Baladi goats after 7 days of parturition where animals were divided into three groups, three animals each, using 3x3 Latin square design. The first group fed control diet (60% CFM and 40% Berseem hay), the second group fed T1 (control diet plus 15 g kg-1, DM), the third group fed T2 (control diet plus 5.85 U kg-1, DM). Tanozym supplementation significantly (p<0.05) increased nutrients digestibility, nutritive values, ruminal Total Volatile Fatty Acids (TVFA’s) but insignificant (p<0.05) increased ammonia nitrogen (NH3 N). Lower significant (p<0.05) values of rumen pH were recorded for treated groups compared with the control. Blood serum of animals fed Tanozym and PEG had higher values of total protein, albumin, globulin, total lipids, urea and glucose but lower values of AST and ALT compared with those of control. Daily milk yield, SNF, lactose and ash yield were significantly (p<0.05) increased with Tanozym compared control group. While, there are no significant (p>0.05) differences among groups for fat corrected milk 4%, total solids, fat and total protein yield.
  A.A. Aboamer , M.S.A. Khattab , S.A.H. Abo El-Nor , H.M. Saleh , A.M. Kholif , I.M. Khattab , M.M. Khorshed and H.M. El-Sayed
  Background: Synchronization the rate of which dry matter and crude protein degraded at the rumen significantly affects the synthesis of microbial protein and thus the efficiency of feed utilization. During previous study a new software application "Lacto-sheep" had been developed to facilitate the formulation of the least cost ration considered this important factor. So that formulation of a synchronous least cost ration is possible and not difficult. This study was carried out to investigate the advantages of feeding synchronous least cost ration as an alternative to the traditional least cost ration on nutrients digestibility and milk production and its constituents. Materials and Methods: The kinetics of dry matter and crude protein disappearance and approximate analysis were determined for each feed ingredients used. Then, using our feed formulation software "Lacto-Sheep" two diets; traditional least cost and synchronous least cost ration were formulated and fed to two groups of multiparous lactating Barki ewes (5 ewes each), suckling single with an average body weight (35.10±2.50 kg) using completely random design. Experiment started 2 weeks after lambing and lasted 60 days. Results: The synchronous least cost ration contained variety of protein sources compared with the least cost ration, which did not contain any rich source of protein (soya bean or cotton seed meal) that’s because the cost of feed is the main limiting factor regardless optimizing feed utilization. Digestion coefficient of crude protein was slightly improved in group fed synchronous least cost ration. In addition, milk production and its components were also slightly higher. However, the differences were not significant. Conclusion: Based on the results obtained from this study, synchronous least cost ration seemed to be more practically appropriate ration for feeding lactating Barki ewes.
  H.H. Azzaz , H.A. Murad , A.M. Kholif , T.A. Morsy , A.M. Mansour and H.M. El-Sayed
  Two experiments were carried out to evaluate the effects of fibrolytic enzymes supplementation on in vitro degradation of sugar beet pulp and in vivo nutrients digestibility, milk yield and its composition by mild-lactating buffaloes. In the in vitro experiment, dry matter and organic matter disappearance (IVDMD and IVOMD) were determined for sugar beet pulp supplemented separately with laboratory produced fibrolytic enzymes (Asperozym) and commercial fibrolytic enzymes source (Tomoko®) at 3 levels (0, 1, 1.5 and 2 g kg-1 DM). Increasing the Asperozym and Tomoko® supplementation levels up to 2 g kg-1 DM exhibited the highest (p<0.05) values of IVDMD and IVOMD. In the in vivo experiment, fifteen mild-lactating buffaloes after 3 months of parturition were divided into three groups, five animals each, using complete random design. The first group was fed on 45% Concentrate Feed Mixture (CFM), 30% corn silage ,15% dried sugar beet pulp and 10% rice straw (control ration). The second group was fed control ration supplemented with Asperozym at 2 g kg-1 DM (R1), while the third group was fed control ration supplemented with Tomoko® at 2 g kg-1 DM. (R2). Asperozym and Tomoko® supplementation significantly (p<0.05) increased DM, OM, CF, NFE, NDF digestibility for treated groups compared with the control group, while blood plasma parameter , milk yield and its composition did not significantly (p<0.05) change among all groups.
  H.H. Azzaz , H.A. Murad , A.M. Kholif , M.A. Hanfy and M.H. Abdel Gawad
  Local isolated Fungal cultures including Aspergillus niger, Fusarium oxysporum, Fusarium avenaceum and Cephalosporium acremonium were employed for cellulase production. The current study aimed at optimization conditions of cellulase production from our local fungal strains. These fungi were grown as stand cultures in 1000 mL conical flasks containing cellulose powder medium for screening their ability for utilizing cellulose as main carbon source for cellulase production. A. niger was chosen on the basis of the best mean cellulase activity reached 0.076 U mL-1, for optimizing culture condition for cellulase production. Wheat straw was used as a sole carbon source for the enzyme production at a concentration of 20% (w/v). The highest activity reached 0.097 U mL-1 was obtained under the optimum conditions of cellulase production including 4% inoculum size, 72 h incubation period, initial pH 6 of the growth medium with using meat extract as a sole nitrogen source at a concentration of 0.33 g L-1 . The result obtained indicated the possibility of cellulase production from A. niger local strain using wheat straw as a sole carbon source.
 
 
 
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