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Research Journal of Microbiology
Year: 2012  |  Volume: 7  |  Issue: 1  |  Page No.: 23 - 31

Optimization of Culture Conditions Affecting Fungal Cellulase Production

H.H. Azzaz, H.A. Murad, A.M. Kholif, M.A. Hanfy and M.H. Abdel Gawad    

Abstract: Local isolated Fungal cultures including Aspergillus niger, Fusarium oxysporum, Fusarium avenaceum and Cephalosporium acremonium were employed for cellulase production. The current study aimed at optimization conditions of cellulase production from our local fungal strains. These fungi were grown as stand cultures in 1000 mL conical flasks containing cellulose powder medium for screening their ability for utilizing cellulose as main carbon source for cellulase production. A. niger was chosen on the basis of the best mean cellulase activity reached 0.076 U mL-1, for optimizing culture condition for cellulase production. Wheat straw was used as a sole carbon source for the enzyme production at a concentration of 20% (w/v). The highest activity reached 0.097 U mL-1 was obtained under the optimum conditions of cellulase production including 4% inoculum size, 72 h incubation period, initial pH 6 of the growth medium with using meat extract as a sole nitrogen source at a concentration of 0.33 g L-1 . The result obtained indicated the possibility of cellulase production from A. niger local strain using wheat straw as a sole carbon source.

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