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Articles by Peng Zhou
Total Records ( 10 ) for Peng Zhou
  Hua Tang , Xin Sheng Liu , Yu Zhen Fang , Li Pan , Zhong Wang Zhang , Peng Zhou , Jian Liang Lv , Shou Tian Jiang , Wen Fa Hu , Pan Zhang , Yong Lu Wang and Yong Guang Zhang
  In recent years, as the genome sequencing technology and protein peptide chemical synthesis technology become mature, especially the proposal of epitopic biology, more and more Foot-and- mouth Disease Virus (FMDV) antigenic epitopes are revealed. Studies on epitopes have greatly promoted the development of new-type vaccines since the early 1980s. Thanks to the consecutive discovery of new epitopes, people can overcome the Major Histocompatibility Complex (MHC) restriction between species more easily. Researchers could also produce poly-vaccine containing epitopes of different serotypes. The selected epitope sequences are highly conserved in different serotypes and this is the key guarantee of cross immune response of vaccine. It is obvious that epitope will play a key role in elaborating new-type FMDV vaccines. This study summarized the epitopes of FMDV.
  Hua Tang , Xin-Sheng Liu , Yu-Zhen Fang , Li Pan , Zhong-Wang Zhang , Peng Zhou , Jian-Liang Lv , Shou-Tian Jiang , Wen-Fa Hu , Pan Zhang , Yong-Lu Wang and Yong-Guang Zhang
  The Foot-and-mouth Disease (FMD), which is the most devastating disease of livestock husbandry, drastically hinders the development of stock raising. At present the main FMD vaccine is still traditional inactivated vaccine, as its good performance in protective activity and immune efficacy, it is widely used in the world for preventing and controlling this disease, especially in developing countries. However, people have realized many disadvantages of inactivated vaccine. In order to resolve these problems, the scientists are conducting some new research about novel FMD vaccine to seek for more secure and effective vaccines. Nowadays, with the rapid development of molecular biology, researchers are paying high emphasis on novel FMD vaccines, such as, synthetic peptide vaccine, epitope-based vaccine, DNA vaccine, chimeric vaccine and so on. There is no doubt that these new-type FMD vaccines will make great contribution to the prevention and control of FMD. This study reviewed the international research progress on the traditional vaccines and novel vaccines of FMD.
  Hua Tang , Xin-Sheng Liu , Yu-Zhen Fang , Li Pan , Zhong-Wang Zhang , Peng Zhou , Jian-Liang Lv , Shou-Tian Jiang , Wen-Fa Hu , Pan Zhang , Yong-Guang Zhang and Yong-Lu Wang
  The Z-curve is a geometrical tool for visualizing and comparing genomes. Since, the curve contains the information carried by the given sequence, DNA sequences could be analyzed systematically. In this study, the ORF5 gene of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) was analyzed by the Z-curve method and the DNAstar (DNASTAR Inc.) computer programs. The results revealed by these two methods were identical. The phylogenetic tree is more digitized and the Z-curve method is more picturesque. It turned out to be that, the Z-curve method shows a widely application prospect in phylogenetic relationships analysis. However, the Z-curve method is still in its premature stage. Novel algorithms are expected to be exploited to extract more information involved in the Z-curves.
  Jiejia Li , Wenyue Guan and Peng Zhou
  Aluminum electrolysis system is a complex industrial process with nonlinear, multivariable, time-varying and large time delay .The mathematical model of it is very difficult to determine, as well as it has a large coupling between the control variable which with high energy consumption. Therefore, research on save power, improve the current efficiency and increase the output and quality of the aluminum electrolysis control system have been become the focus of public concern to be solved. In this paper, by adjusting the controller ’ s control strategy at real-time, controlling the feeding time and feeding rate of alumina, as well as effectively making the alumina concentration is controlled in the range of ideal value, they can make the electrolytic cell at the best working condition. Experimental results showed that this method was effective, increased the current efficiency, improved the control performance, and it had a great significance to raise the output and quality of aluminum.
  Jiejia Li , Xiaoyan Han and Peng Zhou
  According to the characteristics of the aluminum electrolysis fault, the principal component analysis and improved BP network are used for extracting fault feature, the improved BP neural network can extract the fault feature and can also be used as preliminary diagnosis of the fault, thereby electrolytic method for multiple faults diagnosis of three neural networks aluminum is taken use, this method analyzes the deficiency of single neural network and two stage neural network fault diagnosis and in order to form the decision fusion network, the wavelet analysis and neural network are combined organically. The simulation results show that: the aluminum electrolysis fault diagnosis resrarch based on principal component analysis has the characteristic of large amount of ault forecast.
  Hua Tang , Xin-Sheng Liu , Yu-Zhen Fang , Li Pan , Zong-Wang Zhang , Peng Zhou , Jian-Liang Lv , Shou-Tian Jiang , Yong-Lu Wang and Yong-Guang Zhang
  The Z-curve is a geometrical tool for visualizing and comparing genomes. Since, the curve contains the information carried by the given sequence, DNA sequences could be analyzed systematically. In this study, the Foot and Mouth Disease Virus (FMDV) was analyzed by the Z-curve Method. This research concludes that all serotypes FMDV have a close consanguinity but each has its own characteristic by contrast, the three South African serotypes have a much closer affinity. FMDV have three A + T-rich regions and two G + C-rich regions. The most variable regions of FMDV lie in protein coding areas. And the non-coding region is very conservative. O serotype FMDV is most variable compared with A and Asia1 serotype. Those O serotype FMDV in buffalo and goat have a mutation trend. Compared with host, geographic location have a grater influence on FMDV mutation. The A serotype FMDV strains occurred in India after 2002 are more stable and have a closer phylogenetic relationships compared with the Asia1 strains which were prevalent before 2002. In general, Z-curve Method shows a widely application prospect in the areas of life sciences. Such as gene sequence, molecular epidemiology, homology, evolution, phylogenetic relationships and genetic diversity analysis.
  Jiejia Li , Wenyue Guan , Yang Chen and Peng Zhou
  Aluminum electrolysis is a complex industrial process with difficult to control and strong interference, where is difficult to make the aluminum electrolytic cell at the best working condition. Consequently, in this study adopted a fault-tolerant control strategy based on extension neural network according to the characteristics of aluminum electrolysis process. The control principle is adopted different control strategies which combined detection and control, made a diagnosis according to the electrolytic cell condition. It was made the aluminum electrolytic cell in the best working state, difficult to control and strong interference optimized the capability of the system such as real-time ability, stability and precision, real-time ability, stability and precision.
  Zhong- Lu You , Xiao Han , Peng Zhou and Lin Zhang
  A new Schiff-base ligand 2-bromo-4-chloro-6-(cyclopropyliminomethyl)phenol and its zinc(II) complex have been synthesized and characterized by elemental analyses, infrared spectroscopy, 1H NMR, 13C NMR, and single crystal X-ray determinations. The ligand and the complex crystallized in the space groups Pnma and P21/c, respectively. In the complex, the Zn atom is four-coordinate tetrahedral coordination with two imine N and two phenolate O atoms from two Schiff-base ligands. The bond lengths related to the donor atoms in the complex are different from those in the ligand. The coordination of the ligand to the zinc also was supported by IR spectra.
  Wuze Ren , Xiuxia Qu , Wendong Li , Zhenggang Han , Meng Yu , Peng Zhou , Shu-Yi Zhang , Lin-Fa Wang , Hongkui Deng and Zhengli Shi
  Severe acute respiratory syndrome (SARS) is caused by the SARS-associated coronavirus (SARS-CoV), which uses angiotensin-converting enzyme 2 (ACE2) as its receptor for cell entry. A group of SARS-like CoVs (SL-CoVs) has been identified in horseshoe bats. SL-CoVs and SARS-CoVs share identical genome organizations and high sequence identities, with the main exception of the N terminus of the spike protein (S), known to be responsible for receptor binding in CoVs. In this study, we investigated the receptor usage of the SL-CoV S by combining a human immunodeficiency virus-based pseudovirus system with cell lines expressing the ACE2 molecules of human, civet, or horseshoe bat. In addition to full-length S of SL-CoV and SARS-CoV, a series of S chimeras was constructed by inserting different sequences of the SARS-CoV S into the SL-CoV S backbone. Several important observations were made from this study. First, the SL-CoV S was unable to use any of the three ACE2 molecules as its receptor. Second, the SARS-CoV S failed to enter cells expressing the bat ACE2. Third, the chimeric S covering the previously defined receptor-binding domain gained its ability to enter cells via human ACE2, albeit with different efficiencies for different constructs. Fourth, a minimal insert region (amino acids 310 to 518) was found to be sufficient to convert the SL-CoV S from non-ACE2 binding to human ACE2 binding, indicating that the SL-CoV S is largely compatible with SARS-CoV S protein both in structure and in function. The significance of these findings in relation to virus origin, virus recombination, and host switching is discussed.
  Li PAN , Yongguang ZHANG , Yonglu WANG , Jianliang LV , Peng ZHOU , Zhongwang ZHANG , Yuzhen FANG and Shoutian JIANG
  To explore the feasibility of developing a new type of plantderived foot-and-mouth disease virus (FMDV) oral vaccine, the plant seed-specific expression vector p7SBin438/VP1 carrying the VP1 gene of the FMDV strain O/China/99 was constructed and transformed into Agrobacterium tumefaciens strain GV3101. This strain was used for transformation of Arabidopsis thaliana via the floral-dip method. The kanamycin-resistant transgenic plants were selected, and the VP1 gene and protein expressions were confirmed by PCR and sandwich-ELISA, respectively. The ELISA positive plants were further confirmed by Western blot assay, and the VP1 gene in the transgenic progeny was confirmed by PCR. The results showed that the Agrobacterium-mediated floral-dip transformation method could produce transgenic Arabidopsis containing the VP1 gene. Therefore, this protocol provides a successful strategy to deliver viral genes to leguminous plants for genetic studies and for production of low-cost oral vaccines.
 
 
 
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