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Turkish Journal of Veterinary and Animal Sciences
Year: 2011  |  Volume: 35  |  Issue: 4  |  Page No.: 235 - 242

Expression and detection of the FMDV VP1 transgene and expressed structural protein in Arabidopsis thaliana

Li PAN, Yongguang ZHANG, Yonglu WANG, Jianliang LV, Peng ZHOU, Zhongwang ZHANG, Yuzhen FANG and Shoutian JIANG    

Abstract: To explore the feasibility of developing a new type of plantderived foot-and-mouth disease virus (FMDV) oral vaccine, the plant seed-specific expression vector p7SBin438/VP1 carrying the VP1 gene of the FMDV strain O/China/99 was constructed and transformed into Agrobacterium tumefaciens strain GV3101. This strain was used for transformation of Arabidopsis thaliana via the floral-dip method. The kanamycin-resistant transgenic plants were selected, and the VP1 gene and protein expressions were confirmed by PCR and sandwich-ELISA, respectively. The ELISA positive plants were further confirmed by Western blot assay, and the VP1 gene in the transgenic progeny was confirmed by PCR. The results showed that the Agrobacterium-mediated floral-dip transformation method could produce transgenic Arabidopsis containing the VP1 gene. Therefore, this protocol provides a successful strategy to deliver viral genes to leguminous plants for genetic studies and for production of low-cost oral vaccines.

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