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Articles by M.A. Hanfy
Total Records ( 2 ) for M.A. Hanfy
  H.H. Azzaz , A.M. Kholif , H.A. Murad , M.A. Hanfy and M.H. Abdel Gawad
  An in vitro study was conducted to evaluate the effect of cellulases addition to banana wastes on dry matter (IVDMD) and organic matter (IVOMD) disappearances. Laboratory produced cellulase (Asperozym) and a commercial cellulolytic enzyme source (Bacillozym®) were added separately to banana wastes at 4 levels (0, 0.77, 1.54, 2.31 and 3.08 Unit/kg DM). Increasing the Asperozym levels up to 3.08 U kg-1 DM exhibited the highest (p<0.05) IVDMD and IVOMD, while Bacillozym® recorded the highest (p<0.05) IVDMD and IVOMD values at 1.54 U kg-1 DM compared with the untreated banana wastes (Control). Nine lactating Zaraibi goats (about 3 years old and weight on average 31±0.2 kg) after parturition were divided into three groups of three animals each, using 3x3 Latin square designs to evaluate the effect of Asperozym and Bacillozym® addition to diets on the productivity of lactating goats. Animals were fed on 50% Concentrate Feed Mixture (CFM), 25% banana wastes and 25% berseem (clover) straw (control diet). Control diet+ Asperozym at level of 3.08 U kg-1 DM (T1); control diet+Bacillozym® at level of 1.54 U kg-1 DM. (T2). Apparent digestibility for all nutrients were improved (p<0.05) by cellulases treatments. Milk and 4% Fat Corrected Milk (FCM) yields were higher (p<0.05) for T1 group followed by T2 group than control group while milk composition was not affected (p<0.05). Blood plasma Aspartate aminotransferase (AST) and glucose concentration were not affected by treatments. The addition of Asperozym and Bacillozym® to diets improved the performances of lactating Zaraibi goats with no deleterious effects on general health.
  H.H. Azzaz , H.A. Murad , A.M. Kholif , M.A. Hanfy and M.H. Abdel Gawad
  Local isolated Fungal cultures including Aspergillus niger, Fusarium oxysporum, Fusarium avenaceum and Cephalosporium acremonium were employed for cellulase production. The current study aimed at optimization conditions of cellulase production from our local fungal strains. These fungi were grown as stand cultures in 1000 mL conical flasks containing cellulose powder medium for screening their ability for utilizing cellulose as main carbon source for cellulase production. A. niger was chosen on the basis of the best mean cellulase activity reached 0.076 U mL-1, for optimizing culture condition for cellulase production. Wheat straw was used as a sole carbon source for the enzyme production at a concentration of 20% (w/v). The highest activity reached 0.097 U mL-1 was obtained under the optimum conditions of cellulase production including 4% inoculum size, 72 h incubation period, initial pH 6 of the growth medium with using meat extract as a sole nitrogen source at a concentration of 0.33 g L-1 . The result obtained indicated the possibility of cellulase production from A. niger local strain using wheat straw as a sole carbon source.
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