Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by M. Sariah
Total Records ( 6 ) for M. Sariah
  Jugah B. Kadir , A. Ahmad , M. Sariah and A.S. Juraimi
  The possibility of using an indigenous plant pathogen to control itchgrass (Rottboellia cochichinensis) was investigated in this study. A fungal pathogen of itchgrass, Exserohilum longirostratum, was determined in the laboratory and greenhouse as a potential bioherbicide. The disease symptoms on inoculated plants appeared 24 h post inoculation as discrete spot with watery dark border, eventually expand and cause extensive necrosis on the leaves, resulted in burnt-like symptom. When applied as a post emergence foliar spray, the fungus inflicted high percentage of mortality to young itch grass seedlings It did not killed older plants but was capable of reducing biomass by about 56% when the plants were inoculated with 3.5x105 conidia mL-1. Media containing carbon: nitrogen ratio of 10:1 as in V8 juice agar and PDA produced more conidia compared to medium (CMA) containing carbon:nitrogen ratio of 40:1. Light and temperature had major influence on fungal sporulation, exposing the fungus to longer duration (12 h) of light significantly increases conidia production. The optimal temperature for growth and sporulation of this fungus is in the range of 25 to 30°C.
  M.M. Begum , M. Sariah , A.B. Puteh and M.A. Zainal Abidin
  A study on seed-borne infections of soybean [Glycine max (L.) Merrill] demonstrated the infectivity of 17 fungal species belonging to 11 genera using blotter and potato dextrose agar methods. Among them Colletotrichum truncatum, Diaporthe phaseolorum var. sojae and Fusarium oxysporum f. sp. glycines were found in higher frequencies and well established within and on the external surfaces of seeds. Histopathology of C. truncatum infection in seeds of soybean was examined under Light Microscopy (LM) and Scanning Electron Microscopy (SEM). No mycelium and acervulus were observed in any tissues of asymptomatic (healthy) seeds. Seeds colonized with C. truncatum produced irregular gray spots with black specks. C. truncatum produced compact dark mycelium both intra- and intercellularly in the seed coat, cotyledon and embryo. Mycelial growth was more abundant in the hourglass layer of the seed coat and hypodermis, where large inter-cellular spaces were present. Acervuli with setae and abundant hyaline sickle-shaped conidial masses were observed abundantly on the surface of infected seeds. Similar observations were found beneath the inner layers of the seed coat and upper surfaces of embryo and cotyledonary tissues. Brown conidial masses were produced during incubation and liberated in the form of ooze resulting in maceration and disintegration of the parenchyma tissues of the seed coat, cotyledon and embryo.
  M. Maziah , S. Sreeramanan , A. Puad and M. Sariah
  A rice chitinase gene (RCC2) multiplied in Agrobacterium strain (EHA 101), was simultaneously introduced into single buds of in vitro grown banana cultivar, Rastali (AAB). Plasmid pBI333-EN4-RCC2 contained a hygromycin phosphotransferase gene (hpt11) as the selectable marker to identify the transformants. Treatment A contained hygromycin at 25 mg L-1 and treatment B contained hygromycin at 50 mg L-1 in both MS medium supplemented 5 mg L-1 of BAP together with 2.7 g of gelrite agar. Single buds derived from multiple bud clumps (Mbcs), were the target explants for transformation. An assay was performed to identify the minimum concentration required for two antibiotics (carbenicillin and cefotaxime) that is most effective against Agrobacterium strain, EHA 101 and the effect on tissue regeneration capacity. Even though the transformation frequency based on hygromycin selection medium (treatment A) is higher, but there is no transformant could be confirmed based on PCR and Southern blot analyses, as compared using 50 mg L-1 hygromycin selection medium. Assay of protein extract from the transgenic plantlets showed an increased in chitinase enzyme activity over the untransformed plantlets. The present of Agrobacterium-mediated transformation reported here is suitable for using tiny meristem tissues to obtain fungal disease tolerant or resistant banana through genetic engineering.
  M.M. Begum , M. Sariah , M.A. Zainal Abidin , A.B. Puteh and M.A. Rahman
  Histopathological studies on natural infections by Fusarium oxysporum f. sp. glycines in soybean seeds were conducted using Light Microscopy (LM) and Scanning Electron Microscopy (SEM). Asymptomatic (healthy) seeds were found free from pathogens. Infected seeds were slightly irregular in shape and appeared whitish moldy. The fungus mycelia colonized the external surface and inner tissues of the seed coat, but not in cotyledon or in embryo. Upper surfaces of seeds showed profuse colonization by mycelia and seed coat tissues became ruptured and distorted in the severely infected seeds. Vigorous mycelial growth was found in the hourglass layer of the seed coat. Effect of artificial inoculation on soybean seed germination and seedling survivability under glasshouse conditions was also studies. F. oxysporum f. sp. glycines reduced seed germination and seedling survivability by 40% and caused pre-emergence damping off of seedlings. Trichoderma harzianum isolate UPM40 and Pseudomonas aeruginosa isolate UPM13B8 were most effective candidates in inhibiting the mycelial growth of F. oxysporum f. sp. glycines in vitro.
  F.S. Rozlianah and M. Sariah
  .
  F.S. Rozlianah and M. Sariah
  Differentiation of 22 different isolates of Fusarium from tomato fields in Cameron Highlands, based on their cultural and morphological characteristics grouped them into five representative species aggregates; Fusarium oxysporum, F. solani, F. moniliforme, F. chlamydosporum and F. lateritium. This was further confirmed by expression of the DNA polymorphism which showed two main clusters of F. oxysporum and F. solani at the genetic similarity of 56%. The minor clusters include F. moniliforme, F. chlamydosporum and F. lateritium which are not related to F. oxysporum and F. solani. Pathogenicity testing proved the pathogenicity of isolate M1 on tomato. M1 was situated in Cluster I (F. oxysporum) and thus identified as F. oxysporum f. sp. lycopersici. There was no direct relationship between clustering in the RAPD dendrogram and pathogenicity testing of the isolates.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility