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International Journal of Agricultural Research
Year: 2007  |  Volume: 2  |  Issue: 9  |  Page No.: 812 - 819

Detection of Seed-Borne Fungi and Site of Infection by Colletotrichum truncatum in Naturally-Infected Soybean Seeds

M.M. Begum, M. Sariah, A.B. Puteh and M.A. Zainal Abidin    

Abstract: A study on seed-borne infections of soybean [Glycine max (L.) Merrill] demonstrated the infectivity of 17 fungal species belonging to 11 genera using blotter and potato dextrose agar methods. Among them Colletotrichum truncatum, Diaporthe phaseolorum var. sojae and Fusarium oxysporum f. sp. glycines were found in higher frequencies and well established within and on the external surfaces of seeds. Histopathology of C. truncatum infection in seeds of soybean was examined under Light Microscopy (LM) and Scanning Electron Microscopy (SEM). No mycelium and acervulus were observed in any tissues of asymptomatic (healthy) seeds. Seeds colonized with C. truncatum produced irregular gray spots with black specks. C. truncatum produced compact dark mycelium both intra- and intercellularly in the seed coat, cotyledon and embryo. Mycelial growth was more abundant in the hourglass layer of the seed coat and hypodermis, where large inter-cellular spaces were present. Acervuli with setae and abundant hyaline sickle-shaped conidial masses were observed abundantly on the surface of infected seeds. Similar observations were found beneath the inner layers of the seed coat and upper surfaces of embryo and cotyledonary tissues. Brown conidial masses were produced during incubation and liberated in the form of ooze resulting in maceration and disintegration of the parenchyma tissues of the seed coat, cotyledon and embryo.

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