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Research Journal of Microbiology
Year: 2007  |  Volume: 2  |  Issue: 12  |  Page No.: 900 - 909

Histopathological Studies on Soybean Seeds Infected by Fusarium oxysporum f. sp. glycines and Screening of Potential Biocontrol Agents

M.M. Begum, M. Sariah, M.A. Zainal Abidin, A.B. Puteh and M.A. Rahman    

Abstract: Histopathological studies on natural infections by Fusarium oxysporum f. sp. glycines in soybean seeds were conducted using Light Microscopy (LM) and Scanning Electron Microscopy (SEM). Asymptomatic (healthy) seeds were found free from pathogens. Infected seeds were slightly irregular in shape and appeared whitish moldy. The fungus mycelia colonized the external surface and inner tissues of the seed coat, but not in cotyledon or in embryo. Upper surfaces of seeds showed profuse colonization by mycelia and seed coat tissues became ruptured and distorted in the severely infected seeds. Vigorous mycelial growth was found in the hourglass layer of the seed coat. Effect of artificial inoculation on soybean seed germination and seedling survivability under glasshouse conditions was also studies. F. oxysporum f. sp. glycines reduced seed germination and seedling survivability by 40% and caused pre-emergence damping off of seedlings. Trichoderma harzianum isolate UPM40 and Pseudomonas aeruginosa isolate UPM13B8 were most effective candidates in inhibiting the mycelial growth of F. oxysporum f. sp. glycines in vitro.

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