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Articles by Lai L. Suang
Total Records ( 4 ) for Lai L. Suang
  Lai L. Suang , Zamberi Sekawi , Nagi A. Al-Haj , Mariana N. Shamsudin , Rasedee Abdullah and Rahmah Mohamed
  Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study, by using the available B. pseudomallei genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in Burkholderia pseudomallei. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for B. pseudomallei and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen.
  Lai L. Suang , Zamberi Sekawi , Nagi A. Al-Haj , Mariana N. Shamsudin , Rasedee Abdullah and Rahmah Mohamed
  Recently several cases of melioidosis have been reported in the tropical climates, especially in Southeast Asia where, it is endemic, it also occurs sporadically throughout the world. The diagnosis of the acute or chronic infection remains challenging. The present study highlight on the optimized and reliable technique based DNA preparation for use in Polymerase Chain Reaction (PCR) assay. PCR amplification with specific pair of primer for each putative gene was proving specific for amplification of genes in Burkholderia pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. seudomallei specifically for strain D286. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis.
  Nagi A. Al-Haj , Lai L. Suang , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by Lipopolysaccharides (LPS), an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study was designed to further elucidate genes involved in the biosynthesis pathway of LPS in melioidosis agent followed with selected gene product expression with essential function for survival and virulence melioidosis agent. Expression of Bplps0013/lpxA and Bplps0007/rfaF successful expressed the entire proteins in 2 h with sizes of approximately 29 kDa and 43.7 kDa, respectively. The baseline information provided through the present research can be a preliminary approach towards the development of effective therapeutics against melioidosis.
  Nagi A. AL-Haj , Mariana Nor Shamsudin , Raha Abdul Rahim , H. Halimaton , Lai L. Suang , M. Nurmas I. Mashan and Zamberi Sekawi
  Vibrio cholerae has caused severe outbreaks of cholera worldwide with thousands of recorded deaths annually while the Staphylococcus aureus is one of the most significant pathogens causing nosocomial and community-acquired infections. Conventional detection methods for diagnosis of clinical samples, water and food based on culture, microscopy and biochemical testing are limited by the speed of detection, sensitivity and specificity, so it is necessary to develop innovative molecular methods for the rapid detect the presence genes, expression levels of the toxigenic and drug target genes in S. aureus and V. cholerae using PCR, sequencing and membrane array. The genes studied are SEA-SEJ (genes encoding S. aureus enterotoxins) ace, zot, ctxA, ctxB, toxR (toxigenic genes of V. cholerae) Sav1017 and AdaB (protein synthesis and DNA synthesis genes in S. aureus. These techniques were carried out step by step with primers designing, PCR amplification, sequencing and detection of expression by membrane array. These assays are extremely robust, sensitive, specific and economical and can be adapted to different throughputs. Thus, a rapid, sensitive and reliable technique for detecting toxigenic genes of S. aureus and V. cholerae was suc-cessfully developed.
 
 
 
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