Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Research Journal of Applied Sciences
Year: 2009  |  Volume: 4  |  Issue: 2  |  Page No.: 62 - 72

In silico Annotation of the Genes Involved in Biosynthesis of Lipopolysaccharide for Burkholderia pseudomallei

Lai L. Suang, Zamberi Sekawi, Nagi A. Al-Haj, Mariana N. Shamsudin, Rasedee Abdullah and Rahmah Mohamed    

Abstract: Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study, by using the available B. pseudomallei genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in Burkholderia pseudomallei. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for B. pseudomallei and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen.

Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility