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Research Journal of Biological Sciences

Year: 2013  |  Volume: 8  |  Issue: 5  |  Page No.: 155 - 160

Detection the Toxigenic Genes of Vibrio cholerae and Staphylococcus aureus

Nagi A. AL-Haj, Mariana Nor Shamsudin, Raha Abdul Rahim, H. Halimaton, Lai L. Suang, M. Nurmas I. Mashan and Zamberi Sekawi


Vibrio cholerae has caused severe outbreaks of cholera worldwide with thousands of recorded deaths annually while the Staphylococcus aureus is one of the most significant pathogens causing nosocomial and community-acquired infections. Conventional detection methods for diagnosis of clinical samples, water and food based on culture, microscopy and biochemical testing are limited by the speed of detection, sensitivity and specificity, so it is necessary to develop innovative molecular methods for the rapid detect the presence genes, expression levels of the toxigenic and drug target genes in S. aureus and V. cholerae using PCR, sequencing and membrane array. The genes studied are SEA-SEJ (genes encoding S. aureus enterotoxins) ace, zot, ctxA, ctxB, toxR (toxigenic genes of V. cholerae) Sav1017 and AdaB (protein synthesis and DNA synthesis genes in S. aureus. These techniques were carried out step by step with primers designing, PCR amplification, sequencing and detection of expression by membrane array. These assays are extremely robust, sensitive, specific and economical and can be adapted to different throughputs. Thus, a rapid, sensitive and reliable technique for detecting toxigenic genes of S. aureus and V. cholerae was suc-cessfully developed.